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Plasmid#104875PurposepiggyBac vector for cloning 15xQUAS transgenes; used for mosquito tranformationDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceMarch 12, 2018AvailabilityAcademic Institutions and Nonprofits only
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Tornado-RNA sensor for MALAT-1
Plasmid#185406Purposecircular RNA-scaffolded sensing modules for MALAT-1DepositorInsertcircular RNA-scaffolded sensing modules
UseTagsExpressionMammalianMutationWTPromoterU6Available sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/UUG-nLuc-3XFLAG
Plasmid#127302PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from UUG start codonDepositorInsertnanoLuciferase
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
SELP-bio-His
Plasmid#51639PurposeExpresses full-length P-selectin ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertSELP (SELP Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAY7 (OsTIR1 mAID-EGFP-NLS TOL2)
Plasmid#140533PurposeOsTIR1(WT)-P2A-mAID-EGFP-NLS TOL2DepositorInsertmAID-EGFP-NLS TOL2
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV-SFFV-PIK3CA H1047R-WPRE-UbC-Emerald
Plasmid#203798PurposeLentiviral vector expressing human PIK3CA H1047R under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoterDepositorInsertsUseLentiviralTagsExpressionMammalianMutationH1047RPromoterAvailable sinceJuly 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV_UdgX-Anc689-UdgX-nCas9-RBMX
Plasmid#163552PurposeMammalian CG-to-GC base editingDepositorInsertUdgX-Anc689-UdgX-nCas9-RBMX
UseCRISPRTagsExpressionMammalianMutationnCas9 (D10A)PromoterAvailable sinceDec. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQF2WB
Plasmid#61312PurposeEnhancer trap version of QF2, analogous to pGAWBDepositorInsertQF2
UseTagsExpressionInsectMutationPromoterAvailable sinceJan. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
JDW 742 (pDONR221-EGFP-KRAS4B-G12V)
Plasmid#156403PurposeGateway middle entry clone encoding EGFP-fused to human KRAS4B with G12V mutation, includes a stop codonDepositorInserthuman mutant KRAS4B-G12V (KRAS Human)
UseGateway cloningTagsEGFPExpressionMutationmutant KRAS4B-G12VPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLXIN GFP
Plasmid#132935PurposeMLV-based transfer vector expressing GFP and neomycin resistance proteinDepositorInsertGFP
UseLentiviral, RNAi, and RetroviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEM-3XP3.RFP-tan
Plasmid#83474PurposeRFP selectable marker for use in two-stage CRISPR-mediated allele replacement strategies in Drosophila. D. americana tan homology arms can be replaced with locus of choice.DepositorInsert3XP3.RFP-tan
UseCRISPRTagsExpressionBacterial and InsectMutationPromoter3XP3 eye-expression PromoterAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYZ_3WJrep_N20_switch
Plasmid#132722PurposeT7 RNAP-driven expression of 3WJ repressor index 20 switch RNA with a GFP-ASV reporterDepositorInsert3WJ_N20_switch
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYZ_3WJrep_N20_trigger
Plasmid#132726PurposeT7 RNAP-driven expression of 3WJ repressor index 20 trigger RNADepositorInsert3WJ_N20_trigger
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV mouse Dscam-like
Plasmid#18738DepositorInsertDscamL
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
1073A(HomeR1)=pBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#1(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]
Plasmid#159676PurposePlasmid provides the HomeR#1 gene drive element harboring a rescue, gRNA#1, and 3xP3-eGFP that can be integrated via pBac and inserted at Pol-γ35 site #1 via HDR.DepositorInsertpBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#1(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSF4 TetCMV intron Renilla-STOP 24xPP7 1xfirefly-siRNA 24xMS2 SV40 CTE polyA
Plasmid#104097PurposeExpresses TREAT-PK co-localization control in mammalian cellsDepositorInsertRenilla luciferase
UseTags24xMS2 stem loops in 3'UTR and 24xPP7 stem l…ExpressionMammalianMutationPromoterAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV_TALE-TF(VP64)-BB_V3
Plasmid#42581DepositorInsertTALE-TF(VP64)-BB
UseAAV; Adeno-associated virus ()Tags3XFlagExpressionMammalianMutationPromoterCAG hybridAvailable sinceMarch 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCAG-CBE4max-SpCas9-NG-P2A-EGFP (RTW4554)
Plasmid#140001PurposeCAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFPDepositorInserthuman codon optimized CBE4max SpCas9-NG with P2A-EGFP
UseCRISPRTagsP2A-EGFPExpressionMammalianMutationnSpCas9=D10A; SpCas9-NG=L1111R/D1135V/G1218R/E121…PromoterCAGAvailable sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV_NFL(WT)-FLAG
Plasmid#182654PurposeExpresses wild-type mouse neurofilament light chain with a C-terminal FLAG tag (DYKDDDDK) in mammalian cells.DepositorInsertwild type mouse neurofilament light chain (Nefl Mouse)
UseTagsFLAG tag (DYKDDDDK)ExpressionMammalianMutationPromoterCMVAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv1_UQCRC2
Plasmid#177982Purposelentiviral vector expressing Cas9 and a sgRNA targeting UQCRC2DepositorInsertsgRNA targeting UQCRC2 (UQCRC2 Human)
UseLentiviralTagsExpressionMammalianMutationN/APromoterU6Available sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only