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Showing: 8951 - 9000 of 148057 results

  3. pBOB-CAG-SARS-CoV2-Spike-HA

    Plasmid
    #141347
    Purpose
    3. Generation lentiviral vector expressing the codon optimized SARS-CoV2 Spike Glycoprotein ORF with a C-terminal HA tag generated by Junko Ogawa & Gerald M Pao
    Depositor
    Gerald Pao
    Article
    Ogawa et al bioRxiv. 2020 Jul 22. doi: 10.1101/2020.
    Has Service
    Lentiviral Prep
    Insert
    SARS-CoV2 Spike Glycoprotein (S SARS-CoV2 hCoV19_USA EPI_ISL_414366 (GISAID))
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mutation
    resynthesized with human codon optimization nucle…
    Promoter
    CAG
    Available since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    141347-plasmid-map-sequence-id-296391

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  7. pcDNA3.1+CMV-Ins-GLuc

    Plasmid
    #89929
    Purpose
    Expresses Insulin-GLuc from the CMV promoter. Gaussia Luc is inserted within the C-peptide and is co-secreted with insulin.
    Depositor
    Melanie Cobb
    Article
    Kalwat et al ACS Sens. 2016 Oct 28;1(10):1208-1212. E
    Insert
    human insulin-Gaussia-Luciferase (INS Human, Synthetic)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    DNA sequence for Gaussia luciferase was humanized…
    Promoter
    CMV
    Available since
    Sept. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    89929-plasmid-map-sequence-id-255704

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  12. pNTI822

    Plasmid
    #216631
    Purpose
    Tet-inducible cpTurboID (V5) for Sleeping Beauty transposon system
    Depositor
    Nicholas Ingolia
    Article
    Kim et al Nat Chem Biol (2024)
    Insert
    cpTurboID
    Use
    Sleeping beauty transposon system
    Tags
    V5
    Expression
    Mammalian
    Mutation
    L120P
    Promoter
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    216631-plasmid-map-sequence-id-429978

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  19. TFORF2657

    Plasmid
    #144061
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    STAT3 (STAT3 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    144061-plasmid-map-sequence-id-400540

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  20. TFORF2656

    Plasmid
    #143597
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    STAT3 (STAT3 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    143597-plasmid-map-sequence-id-400437

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  27. pCoofy48

    Plasmid
    #55186
    Purpose
    Bacterial expression vector for parallel SLIC cloning containing N-terminal 6x His and GST tags. C-terminal tags can be added during SLIC.
    Depositor
    Sabine Suppmann
    Article
    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.
    Type
    Empty backbone
    Use
    Tags
    6x His, GST, His10-Stop-OneStrep-Stop-S-tag-Stop-…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 26, 2014
    Availability
    Academic Institutions and Nonprofits only
    55186-plasmid-map-sequence-id-85566

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  31. pM9-BetaGal-8C

    Plasmid
    #172489
    Purpose
    Plasmid encoding E. coli BetaGalactosidase with an N-terminal M9 nuclear localization sequence
    Depositor
    Siegfried Musser
    Article
    Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d
    Insert
    M9-BetaGal-8C
    Use
    Tags
    6xHis and M9 nuclear localization sequence (from …
    Expression
    Bacterial
    Mutation
    BetaGal has two cysteine mutations (C77A, C1022S)…
    Promoter
    T7
    Available since
    March 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    172489-plasmid-map-sequence-id-356067

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  32. pDual-eGFP

    Plasmid
    #63215
    Purpose
    Expression of eGFP in bacteria and in mammalian cells. Used as a reporter for quantifying gene targeting and recombineering in mammalian cells.
    Depositor
    Richard S Myers
    Article
    Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1
    Insert
    His-T7-eGFP
    Use
    Lentiviral
    Tags
    His6 and T7
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CMV-EF1α hybrid (CEF)
    Available since
    April 1, 2015
    Availability
    Academic Institutions and Nonprofits only

  33. pCuo DN Rac1

    Plasmid
    #84644
    Purpose
    dominant negative Rac1 under cumate-inducible promoter in Lentiviral vector. Also expresses RFP and puromycin resistance.
    Depositor
    Sanjay Kumar
    Article
    MacKay et al Integr Biol (Camb). 2014 Sep;6(9):885-94
    Insert
    Rac1 (RAC1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Dominant Negative
    Promoter
    cumate-inducible promoter
    Available since
    Dec. 9, 2016
    Availability
    Academic Institutions and Nonprofits only

  35. TFORF1689

    Plasmid
    #143837
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    HNF1A (HNF1A Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    143837-plasmid-map-sequence-id-396268

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  36. p53-GFP

    Plasmid
    #11770
    Depositor
    Geoff Wahl
    Insert
    p53 (TP53 Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    72R variant of p53
    Promoter
    Available since
    May 30, 2006
    Availability
    Academic Institutions and Nonprofits only
    11770-plasmid-map-sequence-id-163545

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  41. pDG459

    Plasmid
    #100901
    Purpose
    SpCas9 with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG and T2A-PuroR
    Expression
    Mammalian
    Mutation
    Promoter
    CBh and U6
    Available since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100901-plasmid-map-sequence-id-197197

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  44. OaAEP1_C247A

    Plasmid
    #200307
    Purpose
    Bacterial expression plasmids for production of C247A mutant of asparaginyl endopeptidase 1 from O. affinis (OaAEP1_C247A)
    Depositor
    Artur Krężel
    Article
    Antonenko et al Bioconjug Chem. 2023 Mar 15;34(4):719-27
    Insert
    Asparaginyl endopeptidase from Oldenlandia affinis
    Use
    Tags
    Smt3
    Expression
    Bacterial
    Mutation
    Cysteine 247 to Alanine
    Promoter
    T7
    Available since
    June 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    200307-plasmid-map-sequence-id-394865

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  48. TFORF3026

    Plasmid
    #144502
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    CREB1 (CREB1 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Sept. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    144502-plasmid-map-sequence-id-407370

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Showing: 8951 - 9000 of 148057 results