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Plasmid#71433PurposeSplit GFP plasmid for visualization of Tau dimerization. Used together with plasmid 71434DepositorInsertTau (0N4R) (MAPT Human)
UseTagsGFP (1-212)ExpressionMammalianMutationPromoterAvailable sinceNov. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTwist-FLAG-SRSF3_Full.Length_Codon.Optimized
Plasmid#172345PurposeExpression of FLAG-tagged, codon-optimized, full-length SRSF3DepositorInsertserine and arginine rich splicing factor 3 (SRSF3 Human)
UseTagsFLAGExpressionMammalianMutationcodon-optimizedPromoterEF-1aAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-UAP56
Plasmid#157661PurposeExpresses UAP56 in E.coli cellDepositorInsertSpliceosome RNA helicase DDX39B (DDX39B Human)
UseTagsGST tagExpressionBacterialMutationdeleted amino acids 1-43PromoterAvailable sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
KIF5C(1-560)-2xmCitrine-GFP11
Plasmid#61667PurposeTandem mCitrine-labeled, truncated kinesin-1 fused to split GFP linkerDepositorInsertKIF5C(1-560)-2xmCitrine-GFP11 (Kif5c Rat)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLKO5d.SFFV.eGFP-miR30n
Plasmid#90333PurposemiR30 based shMir knockdownDepositorInsertseGFP
na
SFFV
miR30n
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pL-CRISPR.SFFV.PAC
Plasmid#57829PurposeLentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin resistance, SFFV Promoter drivenDepositorInsertsSpCas9
Sp sgRNA scaffold
SFFV
P2A-PAC
UseCRISPR and LentiviralTagsFLAGExpressionMutationPromoterAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV-SFFV-RAB10 Q68L-WPRE-UbC-Emerald
Plasmid#203803PurposeLentiviral vector plasmid expressing human RAB10 mutation Q68L (constitutively active mutant) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoterDepositorInsertsUseLentiviralTagsExpressionMammalianMutationQ68LPromoterAvailable sinceJuly 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 3xFLAG TPP1 88-544 delta166-172
Plasmid#53551Purposemammalian expression of TPP1 full length with 166-172 replaced with "GSSG"DepositorInsertTPP1 (ACD Human)
UseTags3xFLAGExpressionMammalianMutationcontains aa 88-544, with aa 166-172 replaced wit…PromoterCMVAvailable sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
CMV_Npu-saBE3.9max N-terminal
Plasmid#137179PurposeExpresses the N-terminal split-intein fragment of S. aureus BE3.9max from the CMV promoterDepositorInsertNpu-saBE3.9max_N-terminal
UseTagsExpressionMammalianMutationCas9 D10APromoterCMVAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO5d.SFFV.dTomato-miR30n
Plasmid#90334PurposemiR30 based shMir knockdownDepositorInsertsdTomato
na
SFFV
miR30n
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
TS-PE2-C-WPRE
Plasmid#176650PurposeExpresses C-terminus of PE2 in mammalian cellsDepositorInsertsC-terminus of PE2
Artificial SA
WPRE
bGH poly(A)
UseAAVTagsSV40 NLSExpressionMammalianMutationPromoterNoneAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTRE-Tight-FLAG-CYLD-C601A
Plasmid#60029PurposeCYLD (C601A) catalytic dead under control of Tet promoter with N-terminal FLAG tagDepositorInsertCylindromatosis (CYLD Human)
UseTagsFLAGExpressionMammalianMutationCysteine 601 changed to AlaninePromoterTREAvailable sinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP-CYLD-C601A
Plasmid#60028PurposeN-terminal GFP tagged catalytic inactive (C601A) expressionDepositorInsertCylindromatosis (CYLD Human)
UseTagsGFPExpressionMammalianMutationCysteine 601 changed to AlaninePromoterCMVAvailable sinceNov. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV-SFFV-RAB10 T23N-WPRE-UbC-Emerald
Plasmid#203802PurposeLentiviral vector plasmid expressing human RAB10 mutation T23N (dominant negative mutant) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoterDepositorInsertsUseLentiviralTagsExpressionMammalianMutationT23NPromoterAvailable sinceJuly 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Prom1 D-5
Plasmid#184017PurposeMammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286). Deletion of exon 19a (amino acids 696-701), and deletion of amino acids 641 to 655.DepositorInsertProm1 SV8(-Ex19a) D-5 (Prom1 Mouse)
UseTagsFlagExpressionMammalianMutationDeletion of exon 19a (amino acids 696-701), and d…PromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
GST-beta2(616-951)
Plasmid#100743PurposeBacterial expression of GST-tagged beta2 subunit of AP2 complex (hinge and ear) long splice formDepositorInsertAP2B1 (AP2B1 Human)
UseTagsGSTExpressionBacterialMutationLong isoformPromoterAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMS74 (synthetic guide::∆HYGR::unc-54 3' UTR^SEC)
Plasmid#154838PurposeInsertion of split hygromycin landing pad into ChrII:8420157 site C. elegans strains via CRISPR with SEC selectionDepositorInsertsynthetic guide::∆HYGR::unc-54 3' UTR
UseCRISPR and Cre/LoxTagsExpressionWormMutation∆HYGR is promoterless and encodes aa 60-341PromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3/flag-pBirA(1-G78)-FRB
Plasmid#154447Purposeexpresses pBirA(1-G78)-FRB/biochemical assayDepositorInsertflag-pBirA(1-G78)-FRB
UseTagsExpressionMammalianMutationPromoterT7 promoterAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ oDam_f_GFPoNLS
Plasmid#85819PurposeiDamID plasmid. To express transiently the optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.DepositorInsertoDam_f_GFPoNLS
UseTagsoNLS (optimized nuclear localization signal) C te…ExpressionBacterial, Insect, Mammalia…MutationL122A. Codon optimization compatible to work in M…PromoterAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
HEL binding scFv D44.1
Plasmid#111718PurposeYeast Surface Display of D44.1 as a reference starting point for designDepositorInsertD44.1
UseTagscmyc tagExpressionYeastMutationPromoterAvailable sinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only