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AoC_pET29b

Plasmid

#215402

Purpose

Expression construct for AoC gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

AoC

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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FsC_pET29b

Plasmid

#215405

Purpose

Expression construct for FsC gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

FsC

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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M-NylC_pET28a

Plasmid

#215409

Purpose

Expression construct for M-NylC gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

M-NylC

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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185_pETcon_SARS2_BQ1-1

Plasmid

#208085

Purpose

yeast surface display of the SARS-CoV-2 BQ.1.1 variant RBD

Depositor

Tyler Starr

Article

Taylor et al PLoS Pathog. 2023 Dec 29;19(12):e1011901

Insert

SARS-CoV-2 BQ.1.1 RBD (S SARS-CoV-2 virus, Budding Yeast)

Use

Tags

HA and c-Myc

Expression

Yeast

Mutation

Promoter

Available since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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186_pETcon_SARS2_XBB1-5

Plasmid

#208086

Purpose

yeast surface display of the SARS-CoV-2 XBB.1.5 variant RBD

Depositor

Tyler Starr

Article

Taylor et al PLoS Pathog. 2023 Dec 29;19(12):e1011901

Insert

SARS-CoV-2 XBB.1.5 RBD (S SARS-CoV-2 virus, Budding Yeast)

Use

Tags

HA and c-Myc

Expression

Yeast

Mutation

Promoter

Available since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pET28b_TypeIC_acrIC4_invivo

Plasmid

#196403

Purpose

expresses acrIC4 protein targeting CRISPR Cascade Type IC for in vivo assay

Depositor

David Taylor

Article

O'Brien et al Mol Cell. 2023 Mar 2;83(5):746-758.e5. d

Insert

anti-CRISPR IC4 protein

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pET28b_TypeIC_acrIF2_invivo

Plasmid

#196402

Purpose

expresses acrIF2 protein targeting CRISPR Cascade Type IC for in vivo assay

Depositor

David Taylor

Article

O'Brien et al Mol Cell. 2023 Mar 2;83(5):746-758.e5. d

Insert

anti-CRISPR IF2 protein (orf30 P. aeruginosa)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pET24a_thyA_R166Q_untagged

Plasmid

#191307

Purpose

Bacterial expression of E. coli thyA (thymidylate synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

thyA

Use

Tags

Expression

Bacterial

Mutation

R166Q TYMS

Promoter

T7

Available since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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pET24a_thyA_Q33S_untagged

Plasmid

#191308

Purpose

Bacterial expression of E. coli thyA (thymidylate synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

thyA

Use

Tags

Expression

Bacterial

Mutation

Q33S TYMS

Promoter

T7

Available since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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pET24a_thyA_R127A_untagged

Plasmid

#191309

Purpose

Bacterial expression of E. coli thyA (thymidylate synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

thyA

Use

Tags

Expression

Bacterial

Mutation

R127A TYMS

Promoter

T7

Available since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET24a_thyA_WT_untagged

Plasmid

#191306

Purpose

Bacterial expression of E. coli thyA (thymidylate synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

thyA

Use

Tags

Expression

Bacterial

Mutation

WT TYMS

Promoter

T7

Available since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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SUMO_LPETG_MT3

Plasmid

#200304

Purpose

Bacterial expression plasmids for sortase mediated purification of recombinant MT3. Contains His-tag for purification and SUMO-tag for better solubility and LPETG for sortase mediated cleavage

Depositor

Artur Krężel

Article

Singh et al Microb Cell Fact. 2023 Jul 11;22(1):125.

Insert

metallothionein 3 (MT3 Human)

Use

Tags

Smt3

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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pET30_Halotag_K73T_Jdomain

Plasmid

#183693

Purpose

Bacterial expression of ER-targeted metastable Halotag/folding sensor (HaloK73T) fused with J domain

Depositor

Edward Avezov

Article

Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

Insert

ER Halo(K73T)-J domain

Use

Tags

Expression

Bacterial

Mutation

K73T in Halotag

Promoter

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pET51b_rHCaMP

Plasmid

#187106

Purpose

Expression of rHCaMP in bacteria

Depositor

Kai Johnsson

Article

Wang et al Angew Chem Int Ed Engl. 2020 Dec 1;59(49

Insert

rHCaMP

Use

Tags

Hisx10 and Strep

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pET51b_hSPR

Plasmid

#187111

Purpose

Expression of hSPR in bacteria

Depositor

Kai Johnsson

Article

Wang et al Angew Chem Int Ed Engl. 2020 Dec 1;59(49

Insert

hSPR

Use

Tags

Hisx10-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pET-PC(1-86)-H6

Plasmid

#183780

Purpose

Express PC(1-86)-H6 in E. coli. PC(1-86)-H6 was used for GST-CBP pull-down assays

Depositor

Peter Harte

Article

Tie et al Proc Natl Acad Sci U S A. 2016 Feb 9;113

Insert

Polycomb (Pc Fly)

Use

Tags

6 His Tag at the C-terminus

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pETM41_KPNA4_Arm

Plasmid

#178478

Purpose

Bacterial expression of human domain with His-tag and MBP tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

KPNA4_Arm (KPNA4 Synthetic, Human)

Use

Tags

His-MBP

Expression

Bacterial

Mutation

Promoter

T7/LacO

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pETM33_ANKRA2_ANK

Plasmid

#178466

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

ANKRA2_ANK (ANKRA2 Synthetic, Human)

Use

Tags

His-GST

Expression

Bacterial

Mutation

Promoter

T7/LacO

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pETM33_DCUN1D1_PONY

Plasmid

#178473

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

DCUN1D1_PONY (DCUN1D1 Synthetic, Human)

Use

Tags

His-GST

Expression

Bacterial

Mutation

Promoter

T7/LacO

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pETM33_CRK_SH3

Plasmid

#178472

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

CRK_SH3 (CRK Synthetic, Human)

Use

Tags

His-GST

Expression

Bacterial

Mutation

Promoter

T7/LacO

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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