186_pETcon_SARS2_XBB1-5
(Plasmid
#208086)
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Purposeyeast surface display of the SARS-CoV-2 XBB.1.5 variant RBD
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 208086 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepETcon
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Vector typeYeast Expression
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Selectable markersTRP1
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSARS-CoV-2 XBB.1.5 RBD
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SpeciesS. cerevisiae (budding yeast); SARS-CoV-2 virus
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Entrez GeneS (a.k.a. GU280_gp02, spike glycoprotein)
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Tags
/ Fusion Proteins
- HA (N terminal on insert)
- c-Myc (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2023.09.11.557279 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
186_pETcon_SARS2_XBB1-5 was a gift from Tyler Starr (Addgene plasmid # 208086 ; http://n2t.net/addgene:208086 ; RRID:Addgene_208086) -
For your References section:
Deep mutational scans of XBB.1.5 and BQ.1.1 reveal ongoing epistatic drift during SARS-CoV-2 evolution. Taylor AL, Starr TN. PLoS Pathog. 2023 Dec 29;19(12):e1011901. doi: 10.1371/journal.ppat.1011901. eCollection 2023 Dec. 10.1371/journal.ppat.1011901 PubMed 38157379