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Plasmid#117629PurposeLevel2 MoClo construct, containing level1 plant expression cassettes for eSaCas9(pEPOR1CB0113) and sgRNA_UUAT1 {AT5G04160}(pEPOR1CB0087)DepositorInsert[35S:eSaCas9(pEPOR1CB0113) ] +[AtU6-26:sgRNA]
UseTagsExpressionPlantMutationPromoterAvailable sinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0012
Plasmid#117533PurposeLevel0 Golden Gate part: CDS, eSaCas9 (no stop codon)DepositorInserteSaCas9 (no stop codon)
UseCRISPRTagsExpressionMutationR499A,Q500K,R654A,G655RPromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
p101_CRISPRai_PiggyBac
Plasmid#213777PurposeExpresses Tet-On inducible CRISPRai orthogonal system with VPR-dSaCas9, dSpCas9-KRAB-BFP, zeocin resistance gene, and Tet-On transactivator. PiggyBac vector.DepositorInsertsVPR-dSaCas9
dSpCas9-KRAB-BFP
zeocin
Tet-On transactivator
UseCRISPRTagsExpressionMutationPromoterEF1a (EF-1-alpha intron a) and Tet_On_TREAvailable sinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0113
Plasmid#117552PurposeLevel 1 Golden Gate Cassette: eSaCas9 expression cassette for dicotyledonous plantsDepositorInsert2x35S+5'UTR OMEGA (pICH51288) + eSaCas9 (no stop codon) (pEPOR0SP0012) + YFP (pICSL50005) + Nos (pICH41421)
UseTagsExpressionPlantMutationPromoter2x35S+5'UTR OMEGA (pICH51288)Available sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR2KN0078
Plasmid#117645PurposeLevel2 MoClo construct, containing level1 plant expression cassettes for eSaCas9(pEPOR1CB0113), sgRNA{AT1G73460}(pEPOR1CB0099) and sgRNA{AT1G73460}(pEPOR1CB0110) to Protein kinase superfamily protein.DepositorInsert[35S:eSaCas9(pEPOR1CB0113) ] +[AtU6-26:sgRNA]
UseTagsExpressionPlantMutationPromoterAvailable sinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-sadCas9-VP64
Plasmid#115790PurposeAn AAV vector expressing S. aureus dCas9 fused to VP64.DepositorInsertSa-dCas9
UseAAVTagsNLS and NLS-VP64ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
SaABEmax
Plasmid#119814PurposeA-to-G base editorDepositorInsertecTadA-ecTadA*(7.10)-nSaCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK3b_019
Plasmid#123800PurposeEncodes dsaCAS9 as a Type 3b part to be used in the MTK systemDepositorInsertSadCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
SaKKH-ABEmax
Plasmid#119815PurposeA-to-G base editorDepositorInsertecTadA-ecTadA*(7.10)-nSaCas9 (KKH)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
SaABE8e
Plasmid#138500PurposeA-to-G base editorDepositorInsertecTadA(8e)-nSaCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
dead Sa Cas9
Plasmid#138162PurposeFor orthogonal R loop assay (use with Sp-derived base editor, Sp gRNA, and Sa gRNA for assay)DepositorInsertdSaCas9
UseTagsNLSExpressionMammalianMutationD10A + N580APromoterCMVAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
BPK2139
Plasmid#65776PurposeHuman expression vector for S aureus Cas9: CAG-humanSaCas9-NLS-3xFLAGDepositorInsertmammalian codon-optimized Staphylococcus aureus Cas9-NLS-3xFlag
UseCRISPRTagsNLS-3xFLAGExpressionMammalianMutationPromoterCAGAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJL-SaBE3
Plasmid#85169PurposeExpresses SaBE3 in mammalian cellsDepositorInsertSaBE3
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
MTK3a_026
Plasmid#123778PurposeEncodes dsaCAS9 tagged with NLS as a Type 3a part to be used in the MTK systemDepositorInsertsadCAS9 (with NLS)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
SaKKH-ABE8e
Plasmid#138502PurposeA-to-G base editorDepositorInsertecTadA(8e)-nSaCas9 (KKH)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
SaABE8e(TadA V106W)
Plasmid#138509PurposeA-to-G base editorDepositorInsertecTadA(8e V106W)-nSaCas9
UseTagsExpressionMammalianMutationTadA-8e V106WPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
SaKKH-ABE8e-dimer
Plasmid#138503PurposeA-to-G base editorDepositorInsertecTadA(WT)-ecTadA(8e)-nSaCas9 (KKH)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
SaABE8e-dimer
Plasmid#138501PurposeA-to-G base editorDepositorInsertecTadA(WT)-ecTadA(8e)-nSaCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
SauKKHCas9 PE2*
Plasmid#169852PurposeMammalian Expression, sauCas9-KKH based prime editorDepositorInsertSaCas9KKH-N580A-M-MLV
UseTagsbpSV40 NLS and SV40 NLS and cmyc-NLS and bpSV40 N…ExpressionMammalianMutationPromotercmvAvailable sinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
3XFLAG-VP64-SadCas9-NLS-VP64
Plasmid#135338Purpose3xFLAG 2xVP64 SadCas9 for single guide cloningDepositorInsertSadCas9
UseTags3xFLAG-VP64-SV40 NLS and NLS-VP64ExpressionMammalianMutationD10A and N580APromoterCMVAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only