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Plasmid#42230PurposeA human codon-optimized SpCas9 and chimeric guide RNA expression plasmid.DepositorArticleHas ServiceCloning Grade DNAInserthumanized S. pyogenes Cas9
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceFeb. 27, 2013AvailabilityAcademic Institutions and Nonprofits only
These results may also be relevant.
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hCent2-pEGFP-C1
Plasmid#29559DepositorInsertCent2 (CETN2 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEGFP Centrin2 (Nigg UK185)
Plasmid#41147DepositorInsertCentrin-2 (CETN2 Human)
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
dsRed-cent2
Plasmid#29523DepositorInsertCent2 (CETN2 Human)
UseTagsdsRedExpressionMammalianMutationPromoterAvailable sinceMay 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-22:CETN2-mTagRFP-T
Plasmid#101781PurposeHomology arms and mTagRFPT-linker sequence for N-terminus tagging of human CETN2DepositorInsertCETN2 Homology Arms with mTagRFPT-linker (CETN2 Human)
UseCRISPR; Donor templateTagsmTagRFPT-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceOct. 31, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEBTet-SNAP-Centrin2
Plasmid#136784PurposeMammalian expression of the centrosomal protein Centrin2 N-terminally fused to SNAP-tagDepositorInsertSNAP-Centrin2 (CETN2 Human, Synthetic)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTetD-SNAP-Centrin2
Plasmid#136785PurposeMammalian expression of the centrosomal protein Centrin2 N-terminally fused to SNAP-tagDepositorInsertSNAP-Centrin2 (CETN2 Human, Synthetic)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTetDBl-CLIP-Centrin2
Plasmid#136834PurposeMammalian expression of the centrosomal protein Centrin2 N-terminally fused to CLIP-tagDepositorInsertCLIP-Centrin2 (CETN2 Human, Synthetic)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTetBl-CLIP-Centrin2
Plasmid#136833PurposeMammalian expression of the centrosomal protein Centrin2 N-terminally fused to CLIP-tagDepositorInsertCLIP-Centrin2 (CETN2 Human, Synthetic)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Solo-mStayGold-CETN2
Plasmid#227292PurposeDonor template for mStayGold insertion into the N-terminus of the CETN2 locus. For centriole visualization. To be co-transfected with sgRNA plasmid px330-CETN2 (Addgene #227291)DepositorInsertCETN2 Homology Arms flanking a mStayGold Tag (CETN2 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterPromoterlessAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
hfCas12Max
Plasmid#195336Purposevector for encoding a human codon-optimized hfCas12Max driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoterDepositorInserthuman codon-optimized hfCas12Max
UseTags3xFlagExpressionMammalianMutationN243R, E336R, D892RPromoterCBh, CMV, hU6Available sinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
dCas12Max-ABE
Plasmid#195335Purposevector for encoding a human codon-optimized dCas12Max-ABE driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoterDepositorInserthuman codon-optimized dCas12Max-ABE
UseTagsExpressionMammalianMutationN243R, E336R, D1049APromoterCBh, CMV, hU6Available sinceMarch 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
dCas12Max-CBE
Plasmid#195337Purposevector for encoding a human codon-optimized dCas12Max-CBE driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoterDepositorInserthuman codon-optimized dCas12Max-CBE
UseTagsExpressionMammalianMutationN243R, E336R, D1049APromoterCBh, CMV, hU6Available sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
px330-Axin1
Plasmid#162543PurposesgRNA targeting Axin1DepositorInsertsgRNA targeting Axin1 (Axin1 Mouse)
UseTagsExpressionMammalianMutationNonePromoterU6Available sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
px330-Kmt2b-4
Plasmid#162538PurposesgRNA targeting Kmt2bDepositorInsertsgRNA targeting Kmt2b (Kmt2b Mouse)
UseTagsExpressionMammalianMutationNonePromoterU6Available sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
px330-Kmt2c-1
Plasmid#162531PurposesgRNA targeting Kmt2cDepositorInsertsgRNA targeting Kmt2c (Kmt2c Mouse)
UseTagsExpressionMammalianMutationNonePromoterU6Available sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
px330-Kmt2c-2
Plasmid#162532PurposesgRNA targeting Kmt2cDepositorInsertsgRNA targeting Kmt2c (Kmt2c Mouse)
UseTagsExpressionMammalianMutationNonePromoterU6Available sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
px330-Kmt2c-3
Plasmid#162533PurposesgRNA targeting Kmt2cDepositorInsertsgRNA targeting Kmt2c (Kmt2c Mouse)
UseTagsExpressionMammalianMutationNonePromoterU6Available sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
px330-Kmt2b-3
Plasmid#162537PurposesgRNA targeting Kmt2bDepositorInsertsgRNA targeting Kmt2b (Kmt2b Mouse)
UseTagsExpressionMammalianMutationNonePromoterU6Available sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
px330-Kmt2b-2
Plasmid#162536PurposesgRNA targeting Kmt2bDepositorInsertsgRNA targeting Kmt2b (Kmt2b Mouse)
UseTagsExpressionMammalianMutationNonePromoterU6Available sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only