Skip to main content
Addgene

pEGFP Centrin2 (Nigg UK185)
(Plasmid #41147)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 41147 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pEGFP-C2
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 5300
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Centrin-2
  • Alt name
    CENT2
  • Alt name
    centrin, EF-hand protein, 2
  • Alt name
    CALT
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    519
  • GenBank ID
    NM_004344.1 NP_004335.1
  • Entrez Gene
    CETN2 (a.k.a. CALT, CEN2)
  • Promoter CMV
  • Tag / Fusion Protein
    • eGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII/BamHI (destroyed during cloning)
  • 3′ cloning site XhoI/SalI (destroyed during cloning)
  • 5′ sequencing primer EGFP-C; CMV-F
  • 3′ sequencing primer SV40-pA-R
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

An additional short protein sequence (LDGTAGPGSTGSR) is present at the C-terminus of Centrin2 due to the cloning strategy used.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP Centrin2 (Nigg UK185) was a gift from Erich Nigg (Addgene plasmid # 41147 ; http://n2t.net/addgene:41147 ; RRID:Addgene_41147)
  • For your References section:

    Plk4-induced centriole biogenesis in human cells. Kleylein-Sohn J, Westendorf J, Le Clech M, Habedanck R, Stierhof YD, Nigg EA. Dev Cell. 2007 Aug;13(2):190-202. 10.1016/j.devcel.2007.07.002 PubMed 17681131