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Plasmid#64846PurposeA lentiviral vector for the expression of mouse Nkx6.2 under doxycyline controlDepositorInsertNkx6.2 (Nkx6-2 Mouse)
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBABE.puro/TO/Flag/HuR.wt
Plasmid#110346PurposeDoxycycline inducible mammalian retroviral expression of HuR (wild type) with FLAG tagDepositorInsertELAV like RNA binding protein 1 (ELAVL1 Human)
UseRetroviralTagsFLAGExpressionMammalianMutationPromotergagAvailable sinceJuly 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO-puro-sh-mFAK B
Plasmid#37018DepositorInsertsh-mFAK B (Ptk2 Mouse)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterhU6Available sinceMay 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRSITEP-puro-shWRN1
Plasmid#125783PurposeDOX-inducible expression of a short-hairpin RNA targeting human WRNDepositorInsertshWRN1 (WRN Human)
UseRNAiTagsExpressionMutationPromoterAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Puro-sgNUAK1
Plasmid#138692PurposeExpresses a human NUAK1-targeting sgRNA and Cas9DepositorInsertsgNUAK1 human (NUAK1 Human)
UseLentiviralTagsExpressionMutationPromoterhU6Available sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 shJUND puro
Plasmid#58698PurposeLentiviral shRNA vector for knockdown of human JUNDDepositorInsertshJUND (JUND Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
Ac5-STABLE2-puro
Plasmid#208032PurposeEnables expression of 2 ORFs (with or without tags) and selection with puromycin in insect cells. Includes Flag-mCherry-T2A-GFP-T2A-puro with Actin5C promoter.DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
tet-pLKO.puro_shHuR CDS
Plasmid#110411PurposeTRCN0000276129 (Target CGAGCTCAGAGGTGATCAAAG), silence human ELAVL1 (HuR) gene, doxycycline inducible, puromycin selectionDepositorInsertELAVL1 (HuR)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterH1/TO (RNA PolIII)Available sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
EMSV-Myf5 (puro)
Plasmid#14711DepositorInsertMyf5 (Myf5 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSR-puro-Exo84
Plasmid#31116DepositorInsertExo84 shRNA (EXOC8 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLX302 OAS2-V5 puro
Plasmid#158642PurposeLentiviral expression vector for constitutive expression of V5-tagged OAS2.DepositorInsertOAS2 (OAS2 Human)
UseLentiviralTagsV5ExpressionMutationPromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBabe-puro-RIPK3
Plasmid#78829Purposeretroviral expression of RIPK3DepositorInsertRIPK3 (RIPK3 Human)
UseRetroviralTagsExpressionMammalianMutationPromoter5'LTRAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pX459-puro-hPKAalpha
Plasmid#185379PurposeFor mammalian expression of guide RNA: caccgTTTGAACGAATCAAGACCCT that targets human PKA subunit alphaDepositorInsertPRKACA (PRKACA Human)
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
p28-2iDP-puro
Plasmid#88893PurposeDual-intron DMD platform plasmid. Co-expresses DMD platform segment, firefly luciferase, puroR and mCherry. Plasmid carries phiC31 and Bxb1 attB sites.DepositorInsertsDual-intron DMD platform
luciferase
puromycin resistance enzyme
mCherry
UseTagsExpressionMammalianMutationTruncated version of the dystrophin protein (tran…PromoterAvailable sinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMSCVpuro-Gcn5(D608A)
Plasmid#63707Purposeretroviral expression of enzyme-dead mouse Gcn5 in mammalian cellsDepositorInsertGcn5 (Kat2a Mouse)
UseRetroviralTagsNoneExpressionMammalianMutationD608A mutationPromoterAvailable sinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMSCVpuro-T7-hAIM2-EGFP
Plasmid#51608PurposeExpress T7 and GFP-tagged hAIM2 in mammalian cellsDepositorInsertAIM2 (AIM2 Human)
UseRetroviralTagsEGFP and T7ExpressionMammalianMutationPromoterMESVAvailable sinceMarch 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-OgNLuc_SV40:PuroR
Plasmid#183038PurposeExpresses the FLAG-tagged OgNLuc (Orange LumiFluor) in mammalian cells.DepositorInsertOgNLuc LumiFluor
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMXs-puro HA-WIPI-1
Plasmid#38273DepositorInsertWD repeat domain phosphoinositide-interacting protein 1 (WIPI1 Human)
UseRetroviralTags3xHAExpressionMammalianMutationPromoterAvailable sinceAug. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
lenticrispr-wt-ltr-puro
Plasmid#173428PurposeExpresses Cas9 and guide RNA, contains intact LTRDepositorInsertS. pyogenes sgRNA cassette
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
lentiguide-puro-wt-ltr
Plasmid#182635PurposeExpresses guide RNA, contains intact LTRDepositorInsertS. pyogenes sgRNA cassette
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti_Puro_DEST mouse_p65promoter_mouse_p65-Clover
Plasmid#124549PurposeLentiviral vector to express p65-Clover under p65 promoter (With puromycin resistance)DepositorInsertp65 promoter- p65 CDS-Clover
UseLentiviralTagsCloverExpressionMammalianMutationPromoterAvailable sinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS-puro shNEDD4L #2
Plasmid#27017DepositorInsertshRNA NEDD4L #2 (NEDD4L Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
DNA-CARzeta-IRESpuro
Plasmid#89345Purpose2nd generation lentiviral vector which expresses DNA-CAR receptor upstream of a IRES-puro cassetteDepositorInsertDNA-CARzeta
UseLentiviralTagsSNAPf tagExpressionMammalianMutationPromoterAvailable sinceJune 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS-puro shNEDD4L #1
Plasmid#27016DepositorInsertshRNA NEDD4L #1 (NEDD4L Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLenti-2X-P2A-Puro
Plasmid#110840PurposeLentiviral vector for constitutive expression of 2X in mammalian cells (codon optimized)DepositorInsertBE3RA-2X
UseLentiviralTagsExpressionMutationD10A and 2 tandem NLS sequences in the XTEN linkerPromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBabepuro3-p18 F37I
Plasmid#24936DepositorInsertp18 (CDKN2C Human)
UseRetroviralTagsExpressionMammalianMutationF37IPromoterAvailable sinceJune 23, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-puro-mycRSK2wt
Plasmid#15827DepositorInsertRibosomal S6 kinase 2 (Rps6ka3 Mouse)
UseRetroviralTagsmycExpressionMammalianMutationwild typePromoterAvailable sinceNov. 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
Flag-mPer2ER-pBABEpuro
Plasmid#61430PurposeExpress mPER2-ER* fusion proteinDepositorInsertmPER2 (Per2 Mouse)
UseRetroviralTagsFlag and mouse estrogen receptor ligand binding d…ExpressionMutationPromoterAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRSITEP-puro-shWRN2
Plasmid#125785PurposeDOX-inducible expression of a short-hairpin RNA targeting human WRNDepositorInsertshWRN2 (WRN Human)
UseRNAiTagsExpressionMutationPromoterAvailable sinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
Neuroplastin-CD4d3+4-bio
Plasmid#73111PurposeEXPRESs plasmid for human erythrocyte surface proteins encoding Neuroplastin with rat CD4d3+4-bioDepositorInsertNeuroplastin (NPTN Human)
UseTagsbiotinylation peptide and ratCD4d3+4ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
EMSV-MyoD (puro)
Plasmid#14710DepositorInsertMyoD (Myod1 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(NT)-PGKpuro2ABFP-W
Plasmid#163169PurposeLentiviral plasmid with non-targeting gRNA, co-expression of BFP tagDepositorInsertNon-targeting
UseCRISPR and LentiviralTagsBFPExpressionMammalianMutationPromoterU6Available sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO-puro-hPPM1A
Plasmid#185382PurposeFor mammalian expression of shRNA: AGGGTAATGGGTTGCGATATG that targets human PPM1ADepositorInsertPPM1A (PPM1A Human)
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO-puro-hPPM1B
Plasmid#185383PurposeFor mammalian expression of shRNA: GAGCAGAAGAGGATGAATTTA that targets human PPM1BDepositorInsertPPM1B (PPM1B Human)
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLX302 ID2-V5 puro
Plasmid#83096PurposeLentiviral vector for constitutive expression of human ID2 with C-terminal V5 tagDepositorInsertID2 (ID2 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
TNF-SBP-mCherry_puromycin
Plasmid#65285PurposeTNF reporter only (no hook) (RUSH system)DepositorInsertTNF (TNF Human)
UseTagsStreptavidin Binding Protein (SBP) and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceJune 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-BC-EF1a-Puro
Plasmid#127170PurposePCR template for EF1a-puro resistance cassetteDepositorInsertNone
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Puro-SIK1
Plasmid#138695PurposeExpresses a human SIK1-targeting sgRNA and Cas9DepositorInsertsgSIK1 human (SIK1 Human)
UseLentiviralTagsExpressionMutationPromoterhU6Available sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
Construct 33 - NFKBRp_Cas9_3xNLS_p2a-puroR
Plasmid#81255PurposeLentiviral construct for building stable cell lines expressing Cas9 in the presence of TNFa via puromycin selectionDepositorInsertCas9-3xNLS
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
R26P-SA-EGFP-puro
Plasmid#26890DepositorInsertEGFP
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceDec. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLD-puro-CcVA
Plasmid#24588DepositorInsertGateway(TM) cassette
UseLentiviralTagsVAExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBabe-puro-mIR-31
Plasmid#26088DepositorInsertmiR-31 (MIR31 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
lentiGuide-Puro.3xBsmBI
Plasmid#196709PurposeBackbone for cloning guide libraries with untranscribed barcodes and a tracrRNA of choiceDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
EMSV-MRF4 (puro)
Plasmid#14713DepositorInsertMRF4 (Myf6 Rat)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
Flag- mCry1ER-pBABEpuro
Plasmid#61429PurposeExpress mCRY1-ER* fusion proteinDepositorInsertmCry1 (Cry1 Mouse)
UseRetroviralTagsFlag and mouse estrogen receptor ligand binding d…ExpressionMutationPromoterAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBabe-puro-mRipk3
Plasmid#78830Purposeretroviral expression of mRIPK3DepositorInsertRIPK3 (Ripk3 Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoter5'LTRAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
shERK2a-mlpx-puro
Plasmid#65229Purposeencodes a shRNA against ERK2DepositorInsertshRNA against ERK2 (MAPK1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGAPTrap-mtagBFP2-IRESMuro
Plasmid#82335PurposeVector targeting the mtagBFP2 gene to the GAPDH locus of human cells. BFP is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.DepositorInsertmtagBFP2-IRESMuro
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSCV Puro-UBC:GFP
Plasmid#68482PurposeRetrovirus expressing GFP driven by UBC promoterDepositorInsertsPuromycin Resistance
GFP
UseRetroviralTagsExpressionMammalianMutationPromoterLTR and UBCAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
ME2/pBABE-puro
Plasmid#49164PurposeFor expression of Malic Enzyme 2 in mammalian cells from retroviral vectorDepositorInsertMalic Enzyme 2 (ME2 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterSV40Available sinceNov. 15, 2013AvailabilityAcademic Institutions and Nonprofits only