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pQCXIN-EF1a-mNeonGreen-P2A-Cas9

Plasmid

#189807

Purpose

Retroviral delivery of Cas9

Depositor

Ellen Rothenberg

Article

Hosokawa et al Immunity. 2018 Jun 19;48(6):1119-1134.e7

Insert

Cas9

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV5-Flag-mEPLIN beta

Plasmid

#40956

Depositor

Scott Maul, Elizabeth Luna

Article

Maul et al Gene. 2001 Jan 10;262(1-2):155-60.

Insert

EPLIN beta (Lima1 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only
pcdna6.2- myc ULK1 4SA

Plasmid

#27631

Depositor

Reuben Shaw

Article

Egan et al Science. 2010 Dec 23. ():.

Insert

mouse myc ULK1 4SA (Myc Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

S467A, S555A, T574A, S637A. Initial M from ULK1 r…

Promoter

Available since

March 16, 2011

Availability

Academic Institutions and Nonprofits only

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pEGFP C1-Eps8 ∆cap

Plasmid

#74786

Purpose

mammalian expression of the capping activity mutant Eps8 fused to GFP

Depositor

Giorgio Scita

Article

Hertzog et al PLoS Biol. 2010 Jun 1;8(6):e1000387. doi

Insert

Eps8 (Eps8 Mouse)

Use

Tags

Expression

Mammalian

Mutation

V689D, L693D

Promoter

Available since

May 3, 2016

Availability

Academic Institutions and Nonprofits only
pCMV5-ALK6-HA

Plasmid

#11769

Depositor

Jeff Wrana

Article

Macias-Silva et al J Biol Chem. 1998 Oct 2. 273(40):25628-3

Insert

ALK6 (Bmpr1b Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2006

Availability

Industry, Academic Institutions, and Nonprofits
pMK282 (mCherry2-Bsr)

Plasmid

#72798

Purpose

C-terminal tagging with mCherry2 using CRIPSR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mCherry2-Bsr

Use

CRISPR

Tags

mCherry2

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

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GluN2B (px330:2-guideCas9)

Plasmid

#169430

Purpose

Expression of Cas9 and 2 guides

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

spCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 21, 2021

Availability

Academic Institutions and Nonprofits only

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pMXs-Utf1

Plasmid

#13369

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

undifferentiated embryonic cell transcription factor 1 (Utf1 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
Anti-Nav1.1 Na+ channel [K74/71R]

Plasmid

#128626

Purpose

Mammalian Expression Plasmid of anti-Nav1.1 Na+ channel (Rat). Derived from hybridoma K74/71.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Nav1.1 Na+ channel (Rat) recombinant mouse monoclonal antibody (Scn1a Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pBS PKC theta

Plasmid

#8426

Depositor

Frederic Mushinski

Article

Mischak et al FEBS Lett 1993 Jul 12;326(1-3):51-5.

Insert

Mouse Protein Kinase C theta (Prkcq Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 22, 2005

Availability

Academic Institutions and Nonprofits only
pBabe-mHSF1-zeo

Plasmid

#17755

Depositor

Susan Lindquist

Article

Dai et al Cell. 2007 Sep 21. 130(6):1005-18.

Insert

HSF1 (Hsf1 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 14, 2008

Availability

Academic Institutions and Nonprofits only
isoform PKC lambda (JCE590)

Plasmid

#89775

Purpose

yeast expression of mouse PKC lambda with yeast PKC1 promoter and ADH1 terminator

Depositor

Juan Carlos Igual

Article

Soriano-Carot et al Nucleic Acids Res. 2014 Jun;42(11):7084-

Insert

PKC lambda (Prkci Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

yeast PKC1 promoter (-600 to -1)

Available since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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pYNL-N1_TUBB5

Plasmid

#65678

Purpose

Expresses beta-tubulin-YNL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

beta-tubulin class V

Use

Luciferase

Tags

YNL

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 18, 2015

Availability

Academic Institutions and Nonprofits only
pBRIT Pax7 TAP

Plasmid

#17528

Depositor

Michael Rudnicki

Article

McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

Insert

Pax7 (Pax7 Mouse)

Use

Cre/Lox and Retroviral

Tags

TAP

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
MSCV-ASNS-OE-IRES-Thy1.1

Plasmid

#192947

Purpose

Asparagine synthetase overexpression in murine cells

Depositor

Ping-Chih Ho

Article

Fernandez-Garcia et al Cell Rep. 2022 Nov 15;41(7):111639. doi:

Insert

Asparagine synthetase (Asns Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

5'LTR

Available since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pBS/KS-mId3sp

Plasmid

#16045

Depositor

Robert Benezra

Article

Jen et al Dev Dyn. 1996 Nov . 207(3):235-52.

Insert

Id3 (Id3 Mouse)

Use

Tags

Expression

Bacterial

Mutation

cDNA nucleotides 365-635

Promoter

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
MSCV PIG Fli1

Plasmid

#66983

Purpose

Retroviral expression plasmid for mouse Fli1 expression; containing bicistronic IRES-GFP

Depositor

Christopher Vakoc

Article

Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

Insert

Fli1 (Fli1 Mouse)

Use

Retroviral

Tags

Expression

Mutation

Promoter

LTR

Available since

July 15, 2015

Availability

Academic Institutions and Nonprofits only

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MSCV PIG Erg

Plasmid

#66984

Purpose

Retroviral expression plasmid for mouse Erg expression; containing bicistronic IRES-GFP

Depositor

Christopher Vakoc

Article

Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

Insert

Erg (Erg Mouse)

Use

Retroviral

Tags

Expression

Mutation

Promoter

LTR

Available since

July 15, 2015

Availability

Academic Institutions and Nonprofits only

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pR I-Ab beta Derp1-117

Plasmid

#113639

Purpose

Insect cell expression vector for I-Ab beta chain fused to Der p 1 117-127 antigenic peptide

Depositor

James Moon

Article

Moon et al Methods Mol Biol. 2018;1799:165-181. doi

Insert

I-Ab beta Derp1-117 (H2-Ab1 Mouse)

Use

Tags

6x His tag, fused to Der p 1 (117-127) peptide vi…

Expression

Insect

Mutation

transmembrane and cytoplasmic domains truncated

Promoter

Metallothionein

Available since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pcdna3 T7 JIP3a

Plasmid

#53457

Purpose

interacts with MAPK signaling module

Depositor

Roger Davis

Article

Kelkar et al Mol Cell Biol. 2000 Feb;20(3):1030-43.

Insert

mitogen-activated protein kinase 8 interacting protein 3 (Mapk8ip3 Mouse)

Use

Tags

T7

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 22, 2014

Availability

Academic Institutions and Nonprofits only