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CN3098-pAAV-eHGT_380h-minBglobin-SYFP2-WPRE3-BGHpA

Plasmid

#208123

Purpose

Direct-expressing SYFP2 in astrocytes AAV Virus

Depositor

Boaz Levi, The Allen Institute for Brain Science

Article

Mich et al bioRxiv 2023.09.20.558718

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

NA

Promoter

Beta Globin minimal promoter

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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AiP1778 - pAAV-AiE2493m-minBG-SYFP2-WPRE-BGHpA

Plasmid

#214544

Purpose

AiE2493m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Bosiljka Tasic, The Allen Institute for Brain Science

Article

Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

Promoter

minBG

Available since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-hM3Dq-mCherry (Cre-OFF)

Plasmid

#166609

Purpose

Encodes Cre-inactivated hM3Dq-mCherry under control of the TRE

Depositor

Martin Myers

Article

Sabatini et al Elife. 2021 Mar 11;10. pii: 66835. doi:

Insert

hM3Dq-mCherry

Use

AAV

Tags

Expression

Mutation

Promoter

tetracycline response element

Available since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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AAV:ITR-EF1aCore-SaCas9-single(U6-sgRNA(backbone))-ITR

Plasmid

#207880

Purpose

AAV vector encoding EF1a core promoter-driven SaCas9 and single sgRNA cloning site w/ the engineered Sa Guide scaffold variant (BbsI for sgRNA cloning).

Depositor

Yeh-Hsing Lao

Article

Lao et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

Type

Empty backbone

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

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AiP981-pAAV-mscRE4-minBGpromoter-EGFP-WPRE-hGHpA

Plasmid

#163484

Purpose

Direct-expressing EGFP AAV Virus. Alias: AiP981 - pAAV-AiE2004m-minBG-EGFP-WPRE-HGHpA

Depositor

Bosiljka Tasic, The Allen Institute for Brain Science

Article

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Insert

EGFP

Use

AAV

Tags

Expression

Mutation

Promoter

Beta Globin minimal promoter

Available since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-RAM-d2TTA::TRE-FLEX-MCS-WPRE-pA

Plasmid

#84467

Purpose

Empty pAAV-CRAM plasmid w. multiple cloning site (MCS)

Depositor

Yingxi Lin

Article

Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

Type

Empty backbone

Use

AAV

Tags

Expression

Mutation

Promoter

pRAM and TRE

Available since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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CN2157-rAAV-eHGT_395h-minBglobin-SYFP2-WPRE3-BGHpA

Plasmid

#208146

Purpose

Direct-expressing SYFP2 in oligodendrocytes AAV Virus

Depositor

Boaz Levi, The Allen Institute for Brain Science

Article

Mich et al bioRxiv 2023.09.20.558718

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

NA

Promoter

Beta Globin minimal promoter

Available since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-mCherry-sesRNA-smFlag-tTA2-W3SL

Plasmid

#192066

Purpose

Expression of mCherry, sesRNA in mammalian cells, with smFlag and tTA2 as efRNA.

Depositor

Josh Huang

Article

Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

Insert

sesRNA for Mouse Ctip2

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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CN2254-rAAV-eHGT_459m-minBglobin-SYFP2-WPRE3-BGHpA

Plasmid

#164454

Purpose

Direct-expressing SYFP2 AAV Virus

Depositor

Jonathan Ting, The Allen Institute for Brain Science

Article

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

Promoter

minBetaGlobin

Available since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-EWB-DIO-myrTagRFP-T-P2A-post-eGRASP

Plasmid

#111581

Purpose

An AAV vector that expresses double floxed myristoylated TagRFP-T and post-eGRASP linked by self-cleaving P2A peptide under the Ef1a promoter.

Depositor

Bong-Kiun Kaang

Article

Choi et al Science. 2018 Apr 27;360(6387):430-435.

Insert

myrTagRFP-T-P2A-post-eGRASP

Use

AAV

Tags

Expression

Mutation

Promoter

Ef1a

Available since

June 12, 2018

Availability

Academic Institutions and Nonprofits only

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AiP1658 - pAAV-AiE2340m-minBG-SYFP2-WPRE-BGHpA

Plasmid

#214509

Purpose

AiE2340m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Bosiljka Tasic, The Allen Institute for Brain Science

Article

Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

Promoter

minBG

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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AiP1858 - pAAV-AiE2538m-minBG-SYFP2-WPRE-BGHpA

Plasmid

#214558

Purpose

AiE2538m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Bosiljka Tasic, The Allen Institute for Brain Science

Article

Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

Promoter

minBG

Available since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSynapsin1-FLEx-axon-GCaMP6s-P2A-mRuby3

Plasmid

#112008

Purpose

For cre-dependent enriched expression of GCaMP6s in axons with cytosolic expression of mRuby3

Depositor

Lin Tian

Article

Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

Insert

axon-GCaMP6s-P2A-mRuby3

Use

AAV and Cre/Lox

Tags

GAP43 palmitoylation domain

Expression

Mammalian

Mutation

Promoter

hSynapsin1

Available since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA

Plasmid

#92392

Purpose

produces AAV expressing Cal-light TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA

Depositor

Hyungbae Kwon

Article

Lee et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

Insert

TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA

Use

AAV and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Human Synapsin

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-DIO eHcKCR1 3.0-oScarlett-KV2.1

Plasmid

#195198

Purpose

all-optical silencing method

Depositor

Karl Deisseroth

Article

Fan et al Cell. 2023 Feb 2;186(3):543-559.e19. doi

Insert

eHcKCR1 3.0-oScarlet-Kv2.1

Use

AAV

Tags

Expression

Mutation

Promoter

CaMKIIa

Available since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

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AiP978-pAAV-mscRE4-minBGpromoter-FlpO-WPRE-hGHpA

Plasmid

#163472

Purpose

Direct-expressing FlpO AAV Virus. Alias: AiP978 - pAAV-AiE2004m-minBG-FlpO-WPRE-HGHpA

Depositor

Bosiljka Tasic, The Allen Institute for Brain Science

Article

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Insert

FlpO

Use

AAV

Tags

Expression

Mutation

Promoter

Beta Globin minimal promoter

Available since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF Con/Fon hChR2(H134R)-EYFP

Plasmid

#55644

Purpose

Cre-on/Flp-on ChR2-EYFP under the short Ef1a promoter

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

ChR2(H134R)-EYFP

Use

AAV; Cre on/flp on chr2-eyfp

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

nEF

Available since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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AiP1657 - pAAV-AiE2339m-minBG-SYFP2-WPRE-BGHpA

Plasmid

#214508

Purpose

AiE2339m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Bosiljka Tasic, The Allen Institute for Brain Science

Article

Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

Promoter

minBG

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato

Plasmid

#51083

Purpose

Cre dependent expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophore

Depositor

Jonathan Ting

Has Service

AAV Retrograde

Insert

GCaMP6f

Use

AAV

Tags

P2A-nls-dTomato

Expression

Mammalian

Mutation

Promoter

hEF1a

Available since

Feb. 27, 2014

Availability

Academic Institutions and Nonprofits only

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CN2014-rAAV-mscRE4-minCMV-SYFP2-WPRE3-BGHpA

Plasmid

#164457

Purpose

Direct-expressing SYFP2 AAV Virus

Depositor

Jonathan Ting, The Allen Institute for Brain Science

Article

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

Promoter

minCMV

Available since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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