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pY010 (pcDNA3.1-hAsCpf1)

Plasmid

#69982

Purpose

Expresses humanized AsCpf1

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

hAsCpf1

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pGTQL1211YN

Plasmid

#61704

Purpose

Gateway compatible BiFC vector (pEarleyGate201-YN)

Depositor

Yuhai Cui

Article

Lu et al Plant J. 2010 Jan;61(2):259-70. doi: 10.

Type

Empty backbone

Use

Bifc assay

Tags

YFP-N and HA

Expression

Plant

Mutation

Promoter

35S

Available since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-8xHis-Ub

Plasmid

#107392

Purpose

Mammalian expression 8xHis-Ub

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

8 tandem repeats of 6xHis-Ub (See comments below)

Use

Tags

None

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV5B-TGFbeta receptor I K232R

Plasmid

#11763

Depositor

Jeff Wrana

Article

Wieser et al EMBO J. 1995 May 15. 14(10):2199-208.

Insert

TGFbeta receptor I (TGFBR1 Human)

Use

Tags

6xHIS

Expression

Mammalian

Mutation

K232R: kinase dead

Promoter

CMV

Available since

May 12, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pGTQL1221YC

Plasmid

#61705

Purpose

Gateway compatible BiFC vector (pEarleyGate202-YC)

Depositor

Yuhai Cui

Article

Lu et al Plant J. 2010 Jan;61(2):259-70. doi: 10.

Type

Empty backbone

Use

Bifc assay

Tags

YFP-C and FLAG

Expression

Plant

Mutation

Promoter

35S

Available since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

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pCR3.1-mGM-CSF

Plasmid

#74465

Purpose

expression of mGM-CSF in mammalian cells

Depositor

Loree Heller

Article

Heller et al Melanoma Res. 2000 Dec;10(6):577-83.

Insert

granulocyte-monocyte colony-stimulating factor (Csf2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 7, 2016

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-syn-jGCaMP8s-WPRE

Plasmid

#162374

Purpose

AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Has Service

AAV Retrograde, AAV1, AAV5, and AAV9

Insert

jGCaMP8s

Use

AAV

Tags

6xHis

Expression

Mammalian

Mutation

S26M F286Y Q315H

Promoter

Synapsin

Available since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pPHT-PIP2

Plasmid

#60937

Purpose

Fluorescent FPX sensor for PIP2

Depositor

Anne Marie Quinn

Article

Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

Insert

PIP2 dimerization dependent sensor RA+B monomers

Use

Tags

dimerization dependent fluorescent protein monome…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pULTRA-CNF

Plasmid

#48215

Purpose

highly efficient suppressor plasmid

Depositor

Peter Schultz

Article

Schultz et al J Am Chem Soc. 2006 Nov 1;128(43):13984-

Inserts

polyspecific MJ tyrosyl synthetase
tRNA

Use

Tags

Expression

Bacterial

Mutation

Y32L, L65V, F108W, Q109M, D158G, I159A

Promoter

proK and tacI

Available since

June 1, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-Sec16A

Plasmid

#36155

Purpose

Expresses Sec16A as a GFP fusion

Depositor

David Stephens

Article

Watson et al Traffic. 2006 Dec;7(12):1678-87. Epub 20

Insert

Sec16A (SEC16A Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-hM4D(Gi)-mCherry

Plasmid

#50477

Purpose

Gi-coupled hM4D DREADD fused with mCherry under the control of CaMKIIa promoter

Depositor

Bryan Roth

Has Service

AAV2, AAV5, AAV8, and AAV9

Insert

hM4D(Gi)-mCherry (CHRM4 Human)

Use

AAV

Tags

mCherry

Expression

Mutation

See supplemental documents for DREADD mutations

Promoter

CaMKIIa

Available since

April 17, 2014

Availability

Academic Institutions and Nonprofits only

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PPRE-H2B-eGFP

Plasmid

#84393

Purpose

Fluorescent reporter for PPARgamma activity

Depositor

Thierry Fournier, Severine Degrelle

Article

Degrelle et al PPAR Res. 2017;2017:6139107. doi: 10.115

Insert

PPARG response element (PPARG Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

PPRE

Available since

Jan. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pCMVHA E2F1

Plasmid

#24225

Purpose

Mammalian expression of human E2F1 with HA tag

Depositor

Kristian Helin

Article

Lukas et al Mol Cell Biol. 1996 Mar . 16(3):1047-57.

Insert

E2F1 (E2F1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 10, 2011

Availability

Academic Institutions and Nonprofits only

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pLenti CMV/TO Puro DEST (670-1)

Plasmid

#17293

Purpose

3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, Puro

Depositor

Paul Kaufman, Eric Campeau

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral; Destination vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pAW12.lentiguide.GFP

Plasmid

#104374

Purpose

GFP vector for cloning in guides

Depositor

Richard Young

Article

Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA flag PPAR gamma

Plasmid

#8895

Purpose

Mammalian expression of flag-tagged PPAR gamma

Depositor

Bruce Spiegelman

Article

Hauser et al J Biol Chem. 2000 Jun 16. 275(24):18527-

Insert

PPAR gamma (Pparg Mouse)

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pLKO.1 hygro shRNA E-cadherin

Plasmid

#18802

Depositor

Bob Weinberg

Article

Onder et al Cancer Res. 2008 May 15. 68(10):3645-54.

Insert

E-cadherin shRNA (CDH1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 22, 2008

Availability

Academic Institutions and Nonprofits only

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pScCas9

Plasmid

#117700

Purpose

Mammalian expression vector encoding WT ScCas9

Depositor

Joseph Jacobson

Article

Chatterjee et al Sci Adv. 2018 Oct 24;4(10):eaau0766. doi

Insert

Streptococcus canis Cas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

EF-1α

Available since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) CircRNA Mini Vector

Plasmid

#60648

Purpose

Expression plasmid for expressing circular RNAs of a desired sequence

Depositor

Jeremy Wilusz

Article

Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

Insert

ZKSCAN1 (ZKSCAN1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1a-eFFly-eGFP

Plasmid

#104834

Purpose

eFFly luciferase for invivo Imaging, eGFP for flourescent detection

Depositor

Irmela Jeremias

Article

Sandhofer et al Leukemia. 2015 Apr;29(4):828-38. doi: 10

Inserts

eFFly
eGFP

Use

Lentiviral and Luciferase; Egfp

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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