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Plasmid#124811PurposeVector for expression Cas9 with gRNA specific for rat IgG2a heavy chain locus. Use in combination with pHybr_r2a>Fab-srt-his plasmids to convert expression of hybridomas to Fab' fragments.DepositorInsertCas9 – gRNA_R2A_hinge
UseCRISPRTags3XFLAG and GFPExpressionBacterial and MammalianMutationR166H in PuroR (please see depositors comment bel…PromoterpUCAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGP-CAG-Ace2N-4AA-mNeon-ST A122D-WPRE-bGH-polyA
Plasmid#172912PurposeMammalian expression of voltage sensor; soma localization targeting sequenceDepositorInsertAce2N-4AA-mNeon-ST A122D
UseTagssoma localization targeting sequenceExpressionMammalianMutationA122DPromoterCAGAvailable sinceNov. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-MAPK1-E322K
Plasmid#116432PurposeLentiviral expression of MAPK1 E322KDepositorInsertMAPK1 (MAPK1 Human)
UseLentiviralTagsExpressionMutationE322KPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-KRAS-Q61R
Plasmid#116425PurposeLentiviral expression of KRAS Q61RDepositorInsertKRAS (KRAS Human)
UseLentiviralTagsExpressionMutationQ61RPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-ERBB2-G776V
Plasmid#116333PurposeLentiviral expression of ERBB2 G776VDepositorInsertERBB2 (ERBB2 Human)
UseLentiviralTagsExpressionMutationG776VPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-ERBB2-S310Y
Plasmid#116350PurposeLentiviral expression of ERBB2 S310YDepositorInsertERBB2 (ERBB2 Human)
UseLentiviralTagsExpressionMutationS310YPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-ERBB2-S310F
Plasmid#116349PurposeLentiviral expression of ERBB2 S310FDepositorInsertERBB2 (ERBB2 Human)
UseLentiviralTagsExpressionMutationS310FPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHybr_r2a>mG1-srt-his
Plasmid#124802PurposeHDR template to knock-in mouse IgG1 Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG1 producing cell lines.DepositorInsertMurine IgG1
UseCRISPR; Hdr templateTagsHistag (HHHHHH) and Sortag (LPETGG)ExpressionBacterial and MammalianMutationPromoter-Available sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-ERBB2-V842I
Plasmid#116359PurposeLentiviral expression of ERBB2 V842IDepositorInsertERBB2 (ERBB2 Human)
UseLentiviralTagsExpressionMutationV842IPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-ERBB2-L755_T759delLRENT
Plasmid#116339PurposeLentiviral expression of ERBB2 L755_T759delLRENTDepositorInsertERBB2 (ERBB2 Human)
UseLentiviralTagsExpressionMutationL755_T759delLRENTPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-KRAS-A146V
Plasmid#116422PurposeLentiviral expression of KRAS A146VDepositorInsertKRAS (KRAS Human)
UseLentiviralTagsExpressionMutationA146VPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-ERBB2-G776delinsVC
Plasmid#116331PurposeLentiviral expression of ERBB2 G776delinsVCDepositorInsertERBB2 (ERBB2 Human)
UseLentiviralTagsExpressionMutationG776delinsVCPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-ERBB2-V777_G778insGSP
Plasmid#116357PurposeLentiviral expression of ERBB2 V777_G778insGSPDepositorInsertERBB2 (ERBB2 Human)
UseLentiviralTagsExpressionMutationP780_Y781insGSPPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-ERBB2-L841V
Plasmid#116341PurposeLentiviral expression of ERBB2 L841VDepositorInsertERBB2 (ERBB2 Human)
UseLentiviralTagsExpressionMutationL841VPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-ERBB2-R678Q
Plasmid#116347PurposeLentiviral expression of ERBB2 R678QDepositorInsertERBB2 (ERBB2 Human)
UseLentiviralTagsExpressionMutationR678QPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-ERBB2-T862A
Plasmid#116354PurposeLentiviral expression of ERBB2 T862ADepositorInsertERBB2 (ERBB2 Human)
UseLentiviralTagsExpressionMutationT862APromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPB-AMBRA1-501*-3xFLAG
Plasmid#174160PurposePiggyBac vector to stably express 3xFLAG-tagged AMBRA1 truncated at amino acid 501DepositorInsertAMBRA1 (AMBRA1 Human)
UseTags3xFLAGExpressionMammalianMutationMissing aa 501-1208PromoterEF1AAvailable sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPB-AMBRA1-217*-3xFLAG
Plasmid#174161PurposePiggyBac vector to stably express 3xFLAG-tagged AMBRA1 truncated at amino acid 217DepositorInsertAMBRA1 (AMBRA1 Human)
UseTags3xFLAGExpressionMammalianMutationMissing aa 217-1208PromoterEF1AAvailable sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-MAP2K4-G294E
Plasmid#116430PurposeLentiviral expression of MAP2K4 G294EDepositorInsertMAP2K4 (MAP2K4 Human)
UseLentiviralTagsExpressionMutationG294EPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHybr_r2a>mA-srt-his
Plasmid#124806PurposeHDR template to knock-in mouse IgA Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgA producing cell lines.DepositorInsertMurine IgA
UseCRISPR; Hdr templateTagsHistag (HHHHHH) and Sortag (LPETGG)ExpressionBacterial and MammalianMutationPromoterAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only