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Showing: 8701 - 8750 of 156346 results

  9. pCW57.1-FOXM1c

    Plasmid
    #68810
    Purpose
    Doxycycline inducible lentiviral vector for FOXM1c expression in mammalian cells
    Depositor
    Adam Karpf
    Article
    Barger et al Oncotarget. 2015 Jul 16.
    Insert
    FOXM1c (FOXM1 Human)
    Use
    Lentiviral; Doxycycline inducible
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    TRE promoter, Tet ON
    Available since
    Sept. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    68810-plasmid-map-sequence-id-232051

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  14. pcDNA3-Clover

    Plasmid
    #40259
    Purpose
    GFP with robust performance in protein fusions and useful as FRET acceptor for mRuby2 in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlap
    Depositor
    Michael Lin
    Article
    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm
    Has Service
    Cloning Grade DNA
    Insert
    Clover GFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV IE
    Available since
    Sept. 24, 2012
    Availability
    Academic Institutions and Nonprofits only
    40259-plasmid-map-sequence-id-215680

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  15. pCAGGs-RFP

    Plasmid
    #127347
    Purpose
    Expresses RFP constitutively via the CAGGS promoter in mammalian cells
    Depositor
    Phil Sharp
    Insert
    Red Fluorescent Protein
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAGGS Promoter
    Available since
    Dec. 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    127347-plasmid-map-sequence-id-246568

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  16. GPX3

    Plasmid
    #38899
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    GPX3 (GPX3 Human)
    Use
    Tags
    Tev His Flag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  18. R777-E134 Hs.MLST8-nostop

    Plasmid
    #70418
    Purpose
    Gateway ORF clone of human MLST8 [NM_022372.4] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    NM_022372.4 (MLST8 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70418-plasmid-map-sequence-id-329035

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  19. HECTD1

    Plasmid
    #32860
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    HECTD1 (HECTD1 Human)
    Use
    Tags
    His (with TEV cleavage)
    Expression
    Bacterial
    Mutation
    amino acids 1879-1966 only
    Promoter
    Available since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  21. pAAV-U6-DR(RfxCas13d)-CAG-hfCas13d-pa

    Plasmid
    #233036
    Purpose
    To express a RfxCas13d-compatible gRNA and to express HA Tagged hfCas13d from a CAG promoter
    Depositor
    Jonathan Ploski
    Insert
    RfxCas13d-compatible gRNA expression cassette and hfCas13d
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    233036-plasmid-map-sequence-id-465997

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  22. pAAV-syn-flex-iGluSnFR4s-PDGFR-WPRE

    Plasmid
    #234439
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Insert
    iGluSnFR4s-PDGFR
    Use
    AAV and Cre/Lox
    Tags
    IgK chain and Myc epi tag-PDGFR TM Domain
    Expression
    Mammalian
    Mutation
    Promoter
    Synapsin
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234439-plasmid-map-sequence-id-465689

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  23. pAAV-syn-flex-iGluSnFR4f-PDGFR-WPRE

    Plasmid
    #234440
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Insert
    iGluSnFR4f-PDGFR
    Use
    AAV and Cre/Lox
    Tags
    IgK chain and Myc epi tag-PDGFR TM Domain
    Expression
    Mammalian
    Mutation
    Promoter
    Synapsin
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234440-plasmid-map-sequence-id-465720

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  24. pAAV-U6-DR30(SapI) 0.5 Syn-intron-hfCas13d-pA

    Plasmid
    #233039
    Purpose
    To express a RfxCas13d-compatible gRNA and to express HA Tagged hfCas13d from a 0.5 bp synapsin promoter
    Depositor
    Jonathan Ploski
    Insert
    RfxCas13d-compatible gRNA expression cassette and hfCas13d
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    233039-plasmid-map-sequence-id-463773

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  25. PGL4.11-COL1A1mSMAD

    Plasmid
    #230917
    Purpose
    Tested the COL1A1 promoter activity after abolition of the proximal SMAD binding sites in the promoter.
    Depositor
    Avi Rosenberg
    Article
    Yang et al Lab Invest. 2024 May;104(5):100336. doi:
    Insert
    Human COL1A1 promoter with mutant SMAD binding sequence (COL1A1 Human)
    Use
    Luciferase
    Tags
    Luciferase-luc2p
    Expression
    Bacterial and Mammalian
    Mutation
    COL1A1 promoter with mutant potential SMAD bindin…
    Promoter
    COL1A1
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230917-plasmid-map-sequence-id-458138

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  26. pCas3

    Plasmid
    #229538
    Purpose
    pMV_hyg encoding Cas3(wt) and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning.
    Depositor
    Kevin Verstrepen
    Article
    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:
    Insert
    Cas3
    Use
    CRISPR
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    codon optimized for S.cerevisiae
    Promoter
    Available since
    Jan. 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    229538-plasmid-map-sequence-id-459007

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  29. pCMV-T7-BPNLS-ABE8e-iSpyMac-BPNLS-P2A-EGFP (LLH528)

    Plasmid
    #208289
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with iSpyMac(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-ABE8e-iSpyMac-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations in TadA; mutations in iSpyMac
    Promoter
    CMV and T7
    Available since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    208289-plasmid-map-sequence-id-410849

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  30. pCHW00019

    Plasmid
    #222448
    Purpose
    Level 0, C terminal tag module (TTCG-GCTT) containing GAL4AD-NLS.
    Depositor
    Chih-Hang Wu
    Article
    Chiang et al bioRxiv 2024.03.23.586378
    Insert
    GAL4AD-NLS
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    no
    Promoter
    Available since
    Dec. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    222448-plasmid-map-sequence-id-453911

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  31. pCHW100003

    Plasmid
    #222442
    Purpose
    Level 0, N-terminal tag module (CCAT-AATG) containing mCherry2-HyP5SM.
    Depositor
    Chih-Hang Wu
    Article
    Chiang et al bioRxiv 2024.03.23.586378
    Insert
    mCherry2-HyP5SM
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    no
    Promoter
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    222442-plasmid-map-sequence-id-454096

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  32. pCHW110033

    Plasmid
    #222441
    Purpose
    Level 0, CDS module (AATG-GCTT) containing Cre recombinase.
    Depositor
    Chih-Hang Wu
    Article
    Chiang et al bioRxiv 2024.03.23.586378
    Insert
    Cre recombinase
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    BsaI/BbsI sites removed by point-mutation
    Promoter
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    222441-plasmid-map-sequence-id-454093

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  33. pCHW111114

    Plasmid
    #222459
    Purpose
    Level 1 acceptor, Position 3 containing CBS4-CMV1::LacZ::nosT.
    Depositor
    Chih-Hang Wu
    Article
    Chiang et al bioRxiv 2024.03.23.586378
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    no
    Promoter
    CBS4-CMV1 (Cucumber mosaic virus)
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    222459-plasmid-map-sequence-id-454092

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  34. pCHW111118

    Plasmid
    #222461
    Purpose
    Level 1 acceptor, Position 3 containing CBS4-CMV1::LoxP-mCherry-stop-LacZ::nosT.
    Depositor
    Chih-Hang Wu
    Article
    Chiang et al bioRxiv 2024.03.23.586378
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    no
    Promoter
    CBS4-CMV1 (Cucumber Mosaic Virus)
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    222461-plasmid-map-sequence-id-454090

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  35. pCHW111117

    Plasmid
    #222460
    Purpose
    Level 1 acceptor, Position 3 containing CBS4-CMV1::LoxP-mCherry-stop-LacZ::nosT.
    Depositor
    Chih-Hang Wu
    Article
    Chiang et al bioRxiv 2024.03.23.586378
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    no
    Promoter
    CBS4-CMV1 (Cucumber mosaic virus)
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    222460-plasmid-map-sequence-id-454089

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  36. pCHW111113

    Plasmid
    #222458
    Purpose
    Level 1 acceptor, Position 3 containing CBS4-CMV1::LacZ::nosT.
    Depositor
    Chih-Hang Wu
    Article
    Chiang et al bioRxiv 2024.03.23.586378
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    no
    Promoter
    CBS4-CMV1 (Cucumber mosaic virus)
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    222458-plasmid-map-sequence-id-454086

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  37. pCHW91013

    Plasmid
    #222450
    Purpose
    Level 1, Position 3 containing CBS4-miniDFR::RUBY::OCS.
    Depositor
    Chih-Hang Wu
    Article
    Chiang et al bioRxiv 2024.03.23.586378
    Insert
    CBS4-miniDFR::RUBY::OCS
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    no
    Promoter
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    222450-plasmid-map-sequence-id-454070

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  38. pCHW100002

    Plasmid
    #222440
    Purpose
    Level 0, CDS module (AATG-GCTT) containing rice OsL5.
    Depositor
    Chih-Hang Wu
    Article
    Chiang et al bioRxiv 2024.03.23.586378
    Insert
    OsL5 (Oryza sativa)
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    BsaI/BbsI sites removed by point-mutation
    Promoter
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    222440-plasmid-map-sequence-id-454069

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  40. pCHW91070

    Plasmid
    #222463
    Purpose
    Level 1 acceptor, Position 3 containing CBS4-CMV1::mCherry2-HyP5SM-LacZ::nosT.
    Depositor
    Chih-Hang Wu
    Article
    Chiang et al bioRxiv 2024.03.23.586378
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    no
    Promoter
    CBS4-CMV1 (Cucumber Mosaic Virus)
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    222463-plasmid-map-sequence-id-454061

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Showing: 8701 - 8750 of 156346 results