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Showing: 8701 - 8750 of 148057 results

  4. pDIRECT_22C

    Plasmid
    #91135
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:npt II
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    July 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    91135-plasmid-map-sequence-id-182261

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  9. pTRE3G-Bi-gePSI-pCMV-AcGFP

    Plasmid
    #131008
    Purpose
    Expresses both the gePSI-α-chain and the gePSI-β-chain under control of the inducible bi-directional TRE3G promoter. Expresses constitutively AcGFP under control of a CMV promoter.
    Depositor
    Erin Schuman
    Article
    Heumuller et al Nat Methods. 2019 Jul 15. pii: 10.1038/s
    Inserts
    gePSI-β-chain
    gePSI-α-chain
    AcGFP1
    Use
    AAV
    Tags
    murine ornithine decarboxylase - degron (ODC36)
    Expression
    Mammalian
    Mutation
    Promoter
    pCMV and pTRE3G-Bi
    Available since
    Dec. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    131008-plasmid-map-sequence-id-266807

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  10. pcDNA3-HA-p48

    Plasmid
    #11614
    Depositor
    Steven Johnson
    Insert
    Interferon-Stimulated Gene Factor 3 (IRF9 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 18, 2007
    Availability
    Academic Institutions and Nonprofits only

  12. pCI-VSVG

    Plasmid
    #1733
    Purpose
    VSV-G expressing envelope vector; recommended for the FELIX (FIV-based) vector system from the Nolan lab
    Depositor
    Garry Nolan
    Insert
    VSV-G Envelope
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 20, 2005
    Availability
    Academic Institutions and Nonprofits only
    1733-plasmid-map-sequence-id-334

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  18. pME-mTagBFP-CAAX

    Plasmid
    #75149
    Purpose
    Membrane targeted TagBFP (blue) fluorophore for use with 5’ promoters.
    Depositor
    Nicholas Cole
    Article
    Don et al Zebrafish. 2016 Sep 15.
    Insert
    mTagBFP-CAAX
    Use
    Middle entry vector for multisite gateway three f…
    Tags
    Expression
    Mutation
    Promoter
    n/a
    Available since
    Oct. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    75149-plasmid-map-sequence-id-142103

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  20. TRE-mClover3-TDP-43ΔNLS

    Plasmid
    #214917
    Purpose
    inducible expression of TDP-43 harboring mutant NLS and N-terminal mClover3. Expression yields cytosolic TDP-43 that forms peinuclear puncta
    Depositor
    Ophir Shalem
    Article
    Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d
    Insert
    TDP-43ΔNLS (TARDBP Human)
    Use
    Lentiviral
    Tags
    mClover3
    Expression
    Mutation
    mutant NLS GCAGCAGCAATGGATGAGACAGATGCTTCATCAGCAGT…
    Promoter
    Available since
    March 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    214917-plasmid-map-sequence-id-424953

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  21. pLEX307-FURIN-puro

    Plasmid
    #158460
    Purpose
    Constitutive lentiviral expression of human FURIN.
    Depositor
    Alejandro Chavez, Sho Iketani
    Insert
    FURIN (FURIN Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158460-plasmid-map-sequence-id-308168

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  22. LiOn*-CAG∞RFP

    Plasmid
    #154019
    Purpose
    Vector based on the LiOn integration-coupled translational switch and devoid of TTAA sequences, expressing the fluorescent protein mRFP1 from a CAG promoter upon action of the piggyBac transposase
    Depositor
    Jean Livet
    Article
    Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d
    Insert
    mRFP1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    All TTAA in vector backbone were replaced by sile…
    Promoter
    CAG
    Available since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    154019-plasmid-map-sequence-id-308117

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  23. pLX-AS

    Plasmid
    #188870
    Purpose
    Compact T-DNA binary vector of the pLX series suitable for assembly of plant virus infectious clones and their Agrobacterium-mediated delivery to plants
    Depositor
    Fabio Pasin
    Article
    Pasin et al STAR Protoc. 2022 Dec 16;3(4):101716. do
    Insert
    none
    Use
    Synthetic Biology; Binary t-dna vector
    Tags
    Expression
    Plant
    Mutation
    Promoter
    cauliflower mosaic virus (CaMV) 35S promoter
    Available since
    Nov. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    188870-plasmid-map-sequence-id-377941

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  31. DENV4 Rluc Reporter GND mutant Replicon

    Plasmid
    #118586
    Purpose
    For use as a positive control for translation of the Rluc gene in the transfected cells and negative control for replication.
    Depositor
    Radhakrishnan Padmanabhan
    Article
    Alcaraz-Estrada et al J Gen Virol. 2010 Nov;91(Pt 11):2713-8.
    Insert
    DENV4 Renilla luciferase gene (Rluc) reporter replicon containing replication-defective GND mutation in the NS5 polymerase gene.
    Use
    Yeast-e. col shuttle vector prs424
    Tags
    Expression
    Mutation
    The conserved GDD in the NS5 polymerase gene muta…
    Promoter
    Available since
    Aug. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    118586-plasmid-map-sequence-id-227283

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  34. pCAG-B19L

    Plasmid
    #59922
    Purpose
    Expression vector for Rabies virus SAD B19 RNA dependent RNA polymerase gene
    Depositor
    Ian Wickersham
    Insert
    Rabies virus RNA dependent RNA polymerase
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Sept. 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    59922-plasmid-map-sequence-id-94980

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  36. pWS175

    Plasmid
    #90962
    Purpose
    Cas9 gap repair vector - HygR
    Depositor
    Tom Ellis
    Insert
    Cas9
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    90962-plasmid-map-sequence-id-287209

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  37. AAV-pCbh-ABE8e-nSpCas9(D10A/A61R)[N-term] (CA19)

    Plasmid
    #197510
    Purpose
    Cbh promoter expression plasmid for N-terminal intein-split AAV construct with N-term of ABE8e-nSpCas9(D10A/A61R) (to be used with SpRY C-term constructs)
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    AAV-[ITR]-pCBh-BPNLS-TadA8e-nSpCas9(D10A/A61R)[N-term]-NpuN-BPNLS-[ITR]
    Use
    AAV and CRISPR
    Tags
    BPNLS and NpuN(intein)-BPNLS
    Expression
    Mutation
    ABE8e mutations in TadA and nSpRY(D10A/A61R)
    Promoter
    Cbh
    Available since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197510-plasmid-map-sequence-id-386692

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  43. LYSO-LRRK2

    Plasmid
    #193582
    Purpose
    Expresses human full-length LRRK2 with a lysosomal targeting sequence from LAMTOR1 (1-39aa) and 3xFLAG on the N-terminus
    Depositor
    Mark Cookson
    Article
    Kluss et al Proc Natl Acad Sci U S A. 2022 Oct 25;11
    Insert
    Leucine-rich repeat kinase 2 (LRRK2 Human)
    Use
    Tags
    LAMTOR1(1-39aa) and 3xFLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    193582-plasmid-map-sequence-id-382173

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  46. TFORF2692

    Plasmid
    #142358
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TEAD1 (TEAD1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    142358-plasmid-map-sequence-id-397200

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  47. pC1-CMV-mito-DAAO

    Plasmid
    #141132
    Purpose
    Expresses DAAO with mitochondrial localization signal in mammalian cells
    Depositor
    Vsevolod Belousov
    Insert
    mito-DAAO
    Use
    Tags
    2 mitochondrial localization signals and 7xHis
    Expression
    Mammalian
    Mutation
    deleted DAAO amino acids 367-368
    Promoter
    CMV
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    141132-plasmid-map-sequence-id-323598

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  49. pEF1a-FB-dCas9-puro

    Plasmid
    #100547
    Purpose
    Expresses N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9 protein
    Depositor
    Jian Xu
    Article
    Liu et al Cell. 2017 Aug 24;170(5):1028-1043.e19.
    Inserts
    N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9
    puromycin
    Use
    CRISPR
    Tags
    FLAG, Biotin-acceptor-site (Avitag)
    Expression
    Mammalian
    Mutation
    Promoter
    Human EF1a
    Available since
    Sept. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    100547-plasmid-map-sequence-id-313417

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Showing: 8701 - 8750 of 148057 results