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We narrowed to 28,583 results for: uros

Showing: 3461 - 3480 of 28583 results

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pGL3-MTS-ND1-W86-L14-G1333C-UGI-Puro

Plasmid

#198850

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND1) by swapping Trp codon TGA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

ND1-W86-L14 TALEN

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-MTS-ND5-W68-L15-G1333C-UGI-Puro

Plasmid

#198858

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND5) by swapping Trp codon TGA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

ND5-W68-L15 TALEN

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-MTS-COX3-W16-L17-G1333C-UGI-Puro

Plasmid

#198846

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(COX3) by swapping Trp codon TGA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

COX3-W16-L17 TALEN

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-MTS-ND3-Q26-L18-G1397C-UGI-Puro

Plasmid

#198854

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND3) by swapping Gln codon CAA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

ND3-Q26-L18 TALEN

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-MTS-ATP6-W68-L16-G1333C-UGI-Puro

Plasmid

#198838

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ATP6) by swapping Trp codon TGA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

ATP6-W68-L16 TALEN

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-MTS-ND4-W24-L18-G1333C-UGI-Puro

Plasmid

#198856

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND4) by swapping Trp codon TGA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

ND4-W24-L18 TALEN

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-MTS-ND2-W30-L16-G1333C-UGI-Puro

Plasmid

#198852

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND2) by swapping Trp codon TGA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

ND2-W30-L16 TALEN

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-MTS-CYTB-W77-L15-G1333C-UGI-Puro

Plasmid

#198848

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(CYTB) by swapping Trp codon TGA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

CYTB-W77-L15 TALEN

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-MTS-COX1-W25-L15-G1333C-UGI-Puro

Plasmid

#198842

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(COX1) by swapping Trp codon TGA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

COX1-W25-L15 TALEN

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-MTS-ND6-W82-L15-G1333N-UGI-Puro

Plasmid

#198860

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND6) by swapping Trp codon TGA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

ND6-W82-L15 TALEN

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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PL752-PTPRO-LA-p2a-tdTomato-hGHpA-PGK-puroR-PTPRO-RA

Plasmid

#191686

Purpose

For knocking p2a-tdTomato into PTPRO locus

Depositor

Yuejun Chen

Article

Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

Insert

PTPRO homology arms and p2a-tdTomato

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-CMV-Flag-PolB(K206A/K244A/T304I)-Puro

Plasmid

#177145

Purpose

Lentiviral vector expressing Flag-PolB(K206A/K244A/T304I) and a puromycin resistance cassette

Depositor

Robert Sobol

Article

Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Neurog2 x2)

Plasmid

#171100

Purpose

CRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Neurog2.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9 (Neurog2 S. pyogenes, Mouse)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Ef1a

Available since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCT-CMV-copGFP-Polß(L301R/V303R/V306R)-puro

Plasmid

#128666

Purpose

mammalian expression of copGFP-Polß(L301R/V303R/V306R: TM) protein with puromcyin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

copGFP

Expression

Mammalian

Mutation

L301R/V303R/V306R

Promoter

CMV

Available since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-Polß(L301R/V303R/V306R)-IRES-puro

Plasmid

#128657

Purpose

mammalian expression of Flag-Polß(L301R/V303R/V306R) protein with puromycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

L301R/V303R/V306R

Promoter

CMV

Available since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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NFATc4-CRISPR-gRNA#exon5-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

Plasmid

#124290

Purpose

sgRNA against huiman NFATc4

Depositor

Katalin Josvay, Csaba Vizler

Insert

NFATc4 nuclear factor of activated T cells (NFATC4 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 3, 2019

Availability

Academic Institutions and Nonprofits only
NFATc4-CRISPR-gRNA#exon3-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

Plasmid

#124289

Purpose

sgRNA against human NFATc4

Depositor

Katalin Josvay, Csaba Vizler

Insert

NFATc4 nuclear factor of activated T cells (NFATC4 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 3, 2019

Availability

Academic Institutions and Nonprofits only
pLEX-uORF-HA-birA*-N-Ras(Q61K/T35A)-IRES-Puro

Plasmid

#120565

Purpose

Expresses HA-birA*-N-Ras Q61K/T35A fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

NRAS (NRAS Human)

Use

Lentiviral

Tags

HA-birA*

Expression

Mammalian

Mutation

Q61K, T35A

Promoter

CMV

Available since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pLEX-uORF-HA-birA*-N-Ras(Q61K/Y40A)-IRES-Puro

Plasmid

#120567

Purpose

Expresses HA-birA*-N-Ras Q61K/Y40A fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

NRAS (NRAS Human)

Use

Lentiviral

Tags

HA-birA*

Expression

Mammalian

Mutation

Q61K, Y40A

Promoter

CMV

Available since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLEX-uORF-HA-birA*-N-Ras(Q61K/E37A)-IRES-Puro

Plasmid

#120566

Purpose

Expresses HA-birA*-N-Ras Q61K/E37A fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

NRAS (NRAS Human)

Use

Lentiviral

Tags

HA-birA*

Expression

Mammalian

Mutation

Q61K, E37A

Promoter

CMV

Available since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge