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Showing: 3441 - 3460 of 20413 results

  1. pYPQ262B-ABE8e

    Plasmid
    #161524
    Purpose
    Gateway entry clone (attL1 & attR5) for CRISPR-zSpRYCas9 ABE8e mediated A-G base editing
    Depositor
    Yiping Qi
    Article
    Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41
    Insert
    TadA8e-zSpRYCas9(D10A)
    Use
    CRISPR; Gateway compatible tada8e-zsprycas9(d10a)…
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    161524-plasmid-map-sequence-id-325557

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  2. pcDNA-LmoCas6-p300

    Plasmid
    #126486
    Purpose
    Encodes L.monocytogenes CRISPR Type I-B Cas6 with N-terminal fusion of Flag epitope and NLS and C-terminal fusion of NLS, human p300 HAT core (aa1048-1664), and Flag tag epitope driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103
    Insert
    LmoCas6-p300
    Use
    CRISPR
    Tags
    Flag and Flag, NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and U6
    Available since
    Sept. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    126486-plasmid-map-sequence-id-246633

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  3. pYPQ262m

    Plasmid
    #161522
    Purpose
    Gateway entry clone (attL1 & attR5) for CRISPR-zSpCas9 ABE7.10 mediated A-G base editing
    Depositor
    Yiping Qi
    Article
    Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41
    Insert
    TadA(wt)-TadA(7.10)-zSpCas9(D10A)
    Use
    CRISPR; Gateway compatible tada(wt)-tada(7.10)-zs…
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    161522-plasmid-map-sequence-id-325551

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  4. pcDNA-CasE-p300-Flag

    Plasmid
    #106275
    Purpose
    Encodes E.coli CRISPR Type I-E CasE with N-terminal fusion of E tag epitope and NLS and C-terminal fusion of NLS, human p300 HAT core (aa1048-1664), and Flag tag epitope driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103
    Insert
    EcoCasE-p300
    Use
    CRISPR
    Tags
    E tag, NLS and Flag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    106275-plasmid-map-sequence-id-247907

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  5. AAV Intein-C-SpyCas9, CMV-AcrIIA4-2xmiR-122 target sites

    Plasmid
    #120295
    Purpose
    AAV Vector for expression of C-terminal SpyCas9 fragement with split-intein and a CMV-driven AcrIIA4 with two miR-122 binding sites
    Depositor
    Dominik Niopek
    Article
    Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545
    Insert
    C-terminal fragment of SpyCas9
    Use
    AAV and CRISPR
    Tags
    split-intein
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    120295-plasmid-map-sequence-id-232737

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  10. pJAT32

    Plasmid
    #204297
    Purpose
    pJAT plasmid with multiple cloning site for introducing custom payload. Two U6 promoters.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    204297-plasmid-map-sequence-id-405508

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  11. pUCsRNAP

    Plasmid
    #182746
    Purpose
    Swapping in small RNA promoter, 23-bp spacer sequence, & 19-bp repeats into pJC005 plasmids
    Depositor
    James Collins
    Article
    Chua et al Microbiol Spectr. 2022 Feb 2:e0242721. d
    Insert
    small RNA promoter, a 23-bp spacer sequence, & 19-bp repeats
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    182746-plasmid-map-sequence-id-358079

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  12. pHdzCas9-KRAB

    Plasmid
    #92341
    Purpose
    Expression dzCas9-KRAB in microalgae. CRISPR/Cas based plant genome editing and gene regulation, Hyg resistance
    Depositor
    I-Son Ng
    Article
    Kao et al Bioresour Technol. 2017 May 4. pii: S096
    Type
    Empty backbone
    Use
    CRISPR; Plant expression
    Tags
    KRAB
    Expression
    Plant
    Mutation
    Promoter
    CaMV35S
    Available since
    June 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    92341-plasmid-map-sequence-id-181654

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  13. pLV hUbC- VP64-dCas9-VP64-T2A- GFP(No LoxP sites)

    Plasmid
    #66708
    Purpose
    expresses VP64-dCas9-VP64, same plasmid as Addgene 59791 but with LoxP sites removed
    Depositor
    Charles Gersbach
    Insert
    S.Pyogenes VP64-dCas9-VP64
    Use
    Lentiviral and Synthetic Biology
    Tags
    VP64
    Expression
    Mutation
    D10A and H840A
    Promoter
    hUbC
    Available since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    66708-plasmid-map-sequence-id-114015

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Showing: 3441 - 3460 of 20413 results