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Addgene

pLenti SpBsmBI sgRNA Puro
(Plasmid #62207)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 62207 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pLV
  • Backbone manufacturer
    Addgene plasmid number: 36297
  • Vector type
    Mammalian Expression, Lentiviral
  • Promoter U6
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti SpBsmBI sgRNA Puro was a gift from Rene Maehr (Addgene plasmid # 62207 ; http://n2t.net/addgene:62207 ; RRID:Addgene_62207)
  • For your References section:

    Transcriptional Regulation with CRISPR/Cas9 Effectors in Mammalian Cells. Pham H, Kearns NA, Maehr R. Methods Mol Biol. 2016;1358:43-57. doi: 10.1007/978-1-4939-3067-8_3. 10.1007/978-1-4939-3067-8_3 PubMed 26463376