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Purpose(Empty Backbone) An empty sgRNA expression vector for expression of sgRNA for Sp Cas9 (3rd generation lentiviral vector)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 62207 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLV
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Backbone manufacturerAddgene plasmid number: 36297
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Vector typeMammalian Expression, Lentiviral
- Promoter U6
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberUnknown
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti SpBsmBI sgRNA Puro was a gift from Rene Maehr (Addgene plasmid # 62207 ; http://n2t.net/addgene:62207 ; RRID:Addgene_62207) -
For your References section:
Transcriptional Regulation with CRISPR/Cas9 Effectors in Mammalian Cells. Pham H, Kearns NA, Maehr R. Methods Mol Biol. 2016;1358:43-57. doi: 10.1007/978-1-4939-3067-8_3. 10.1007/978-1-4939-3067-8_3 PubMed 26463376