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Plasmid#218775PurposeHis-TEV-Cas12aUltra-BPSV40_cMyc_NP_5NLSDepositorInsert5xNLS-BPSV40-NLP-cMyc-cMyc-BPSV40- AspCas12a
UseCRISPRTags6XHis (C terminal), HA, Nucleoplasmin, SV40, and …ExpressionBacterialMutationNucleotides mutations were made to encode for arg…PromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
CyPET-Arf6
Plasmid#18840DepositorInsertCyPET-Arf6 (ARF6 Human)
UseTagsExpressionMammalianMutationThe sequence for the fluorescent protein CyPET wa…PromoterAvailable sinceAug. 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLVX-puro_G-CEPIA1er
Plasmid#164590PurposeStable integration and expression of G-CEPIA1er in mammalian cellsDepositorInsertG-CEPIA1er
UseLentiviralTagsMycExpressionMammalianMutationG-GECO1.1 E299D M304L D328N N345K F360W G369S E37…PromoterCMVAvailable sinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2-MT GLI2 FL
Plasmid#17648DepositorInsertGLI2 (GLI2 Human)
UseTags6x MycExpressionMammalianMutationPromoterCMV IE94Available sinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
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pET28a_Pro-IL-36γ
Plasmid#214345PurposeProtein overexpression in bacteriaDepositorInsertIL36G (IL36G Human)
UseTagsHis-TEV and MycExpressionBacterialMutationPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-21a-PE2-His
Plasmid#201973PurposeExpress His-tagged PE2 in bacteriaDepositorInsertPE2-HIS
UseTagsHis and c-myc NLSExpressionBacterialMutationPromoterAvailable sinceJune 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2-MT GLI2 delta N
Plasmid#17649DepositorInsertGLI2 (GLI2 Human)
UseTags6x MycExpressionMammalianMutationMissing 328 N-terminal amino acidsPromoterAvailable sinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSF3-microID
Plasmid#172880Purposeexpresses microID in mammalian cells with the tet-on or tet-off systemDepositorInsertmicroID
UseLuciferase and Retroviral; To be used in a tet-on…TagsmycExpressionMammalianMutationfragment ([aa2-171]) of aquifex aeolicus BirA wit…PromoterTRE-cmvAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a_Pro-IL-36β
Plasmid#214344PurposeProtein overexpression in bacteriaDepositorInsertIL36B (IL36B Human)
UseTagsHis-TEV and MycExpressionBacterialMutationPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
GG-EBNA-OSK2M2L1-PP
Plasmid#102902PurposeEBNA episome plasmid for U6 promoter-driven expression of 10 gRNAs targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters. Includes PGK-puro selection cassette.DepositorInsertOSK2M2L1-gRNAs-PGK-puro
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
ABI_cTEVp
Plasmid#119214PurposeExpresses C-terminus of tobacco etch virus protease (TEVp) fused to ABI-abscisic acid responsive protein domainDepositorInsertsplit cTEVp in fusion with ABI
UseTagsMyc tagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hPGK-eGFP-SynaptoZip
Plasmid#122523PurposeLentiviral expression of the synaptic activity-marker SynaptoZip fused to eGFP driven by hPGK promoterDepositorInserteGFP-SynaptoZip (Vamp2 Rat)
UseLentiviralTagsMyc and eGFPExpressionMammalianMutationPromoterhPGKAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-FLEX-VAMP2:HRP
Plasmid#60659PurposeLabels synaptic vesicles for electron microscopyDepositorInsertVAMP2:HRP
UseAAV and Cre/LoxTagsMycExpressionMutationPromoterCAGAvailable sinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
GST-BHMT-I/G
Plasmid#104442PurposeAutophagy cargo assayDepositorInsertBHMT (BHMT Human)
UseTagsGFP-myc GFP is from Aequorea victoria and GSTExpressionMammalianMutationN43A in GST (in a potential KFERQ-like sequence).…PromoterCMVAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAT15547_PEmax-SuperFi-Cas9
Plasmid#184377PurposeMammalian expression of SuperFi-Cas9 PEmax prime editorDepositorInsertPEmax-SuperFi-Cas9
UseCRISPRTagsSV40 NLS and c-myc NLSExpressionMammalianMutationH840A, R221K, N394K, Y1010D, Y1013D, Y1016D, V101…PromoterCMVAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-synaptoPAC-minWPRE
Plasmid#153085PurposeExpresses synaptoPAC, a fusion of synaptophysin, mScarlet, and bPAC in neuronsDepositorInsertsUseAAVTagsHis tag, mScarlet, and myc tagExpressionMammalianMutationPromoterAvailable sinceSept. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO-VMAT2-WPRE-pA
Plasmid#39339DepositorInsertSlc18a2 (Slc18a2 Mouse)
UseAAV; RTagsExpressionMutationMyc tag inserted in the first lumenal loop betwee…PromoterAvailable sinceOct. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
EGFP in pDBEcR-pIND(SP1)-DD/Bla
Plasmid#201559PurposeEcdysone-inducible vector with SB ITRs for expressing FLAG- EGFP-DD and DBEcR (3xmyc-tagged); blasticidin resistance.DepositorInsertDBEcR and EGFP
UseUnspecified; Sleeping beauty transposonTagsDBEcR-myc, FLAG-EGFP, and FLAG-EGFP-DDExpressionMammalianMutationPromoterEF1a and pIND(SP1)Available sinceJuly 13, 2023AvailabilityAcademic Institutions and Nonprofits only