Showing: 3441 - 3460 of 4263 results
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Plasmid#112146PurposeMammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activityDepositorInsertLuciferase coding region with wild type Cysteine 258 and wild-type PHGPx SECIS in the 3' UTR (Gpx4 Rat, Photinus pyralis)
UseTagsExpressionMammalianMutationnonePromoterAvailabilityAcademic Institutions and Nonprofits only -
pSF4 TetCMV ATF4(5'UTR)-SunTag-Renilla-FKBP-Stop-24xMS2v5-CTE-polyA
Plasmid#164615PurposeExpresses ATF4(5'UTR)-SunTag-Renilla mRNA reporter under tetracycline-inducible promoter. SunTag and Renilla luciferase are in frame with the main start codon of ATF4.DepositorInsertRenilla luciferase
UseTags24xMS2v5 (3'UTR) and SunTagExpressionBacterial and MammalianMutationPromoterTetCMVAvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-Large BiT - SNAP-tag - Beta-actin
Plasmid#171019PurposeLentiviral expression of a Large BiT-SNAP-tag-Beta-actin fusion in mammalian cellsDepositorInsertLargeBit-SNAPtag- Beta-actin
UseLentiviral and LuciferaseTagsBeta actin and LargeBiTExpressionMammalianMutationPromoterEF1AvailabilityAcademic Institutions and Nonprofits only -
pET28a-his-Gluc-iLID
Plasmid#172096PurposeExpresses the Gluc-iLID fusion protein in bacteriaDepositorInsertGaussia Luciferase - iLID
UseTagsExpressionBacterialMutationGluc Mutations - M43L, M110LPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
GLuc-FLAG-CB1
Plasmid#158066PurposeMembrane expression detection of Cannabinoid receptor 1DepositorInsertCB1 (CNR1 Human)
UseAAVTagsGaussia LuciferaseExpressionMammalianMutationcontains 4Kb of 3' UTRPromoterpCAGAvailabilityAcademic Institutions and Nonprofits only -
pKL123
Plasmid#187889PurposeAAVS1 safe-harbor integration of dox-inducible NFIA-SOX9DepositorInsertNFIA-SOX9
UseTagsExpressionMammalianMutationPromoterTRE3GAvailabilityAcademic Institutions and Nonprofits only -
pTS2308-Tier1-pGL3-PPGK-NLuc_TB
Plasmid#169551PurposeTier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' insulator sequence derived from the pGL3 plasmid (pGL3-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).DepositorInsertpGL3-insulator-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
UseTagsExpressionMammalianMutationPromoterPPGKAvailabilityAcademic Institutions and Nonprofits only -
p406 – TDH3-ER-FlucDM-GFP11-Ura
Plasmid#177731PurposeDM Firefly luciferase reporter fused to a KAR2 signal sequence for ER targeting and HDEL for ER retention with an HA tag and the eleventh β-strand of GFP added to the C-terminus in yeast plasmid withDepositorInsertFlucDM
UseTagsGFP11, HA, HDEL, and KAR2 Signal SequenceExpressionBacterial and YeastMutationR188Q, R261Q (Destabilized Mutant)PromoterAvailabilityAcademic Institutions and Nonprofits only -
pFBP_2xNbLYS1::LUZ
Plasmid#189918PurposeBinary vector for Agrobacterium-mediated transient expression of a bioluminescent reporter for assessing immune activation in Nicotiana benthamiana by transiently expressed plant immune receptors.DepositorInsertpLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pAtUbi1:NnH3H:tAtuNos, pCmYLCV:NnHisps:tHsp, p2xNbLYS1:NnLuz:tAtuOcs
UseLuciferaseTagsExpressionPlantMutationPromoterVariousAvailabilityAcademic Institutions and Nonprofits only -
ABHD2-3'UTR-WT
Plasmid#190094Purposeexamine the activity of ABHD2 3'UTRDepositorInsertABHD2 3' UTR Wild Type (ABHD2 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
lentiTet-OTX2-P2A-mCherry BlastR
Plasmid#186741PurposeDox inducible OTX2-P2A-mCherry expression construct cloned into a lentiviral backbone containing a blasticidin resistance geneDepositorInsertOTX2 (OTX2 Human)
UseLentiviralTagsP2A-mCherryExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
p2T CAG dCas9-10XGcn4-P2A-mCherry BlastR
Plasmid#186745PurposedCas9-10XGcn4-P2A-mCherry expression constructDepositorInsertCas9-10XGcn4-P2A-mCherry
UseCRISPRTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pNHT7::UMSBP::sNluc2_ORF2
Plasmid#186766PurposeAn mRNA production vector encoding planarian codon-optimized sNluc2 with UMSBP 5' and 3' UTRs beginning at a start codon further downstream of the endogenous gene's start codon..DepositorInsertNanoluciferase
UseTagsExpressionMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
Luc-T20
Plasmid#177941PurposeTransient mammalian expression of Luc2 (firefly luciferase) along with SARS-CoV-2 sequence 20080-22222 (T20) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.DepositorInsertLuc2 (ORF1ab )
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Luc-PS9
Plasmid#177942PurposeTransient mammalian expression of Luc2 (firefly luciferase) along with SARS-CoV-2 sequence 20080-21171 (PS9) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.DepositorInsertLuc2 (ORF1ab )
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pT2/GD-IRES-GFP-CTNNB1
Plasmid#192868PurposeCarries a Sleeping Beauty (SB) transposon vector that can be used to deliver an activated human CTNNB1(S33Y) transgene into cells, when a source of SB transposase is also co-introduced.DepositorInsertsluc+
EGFP
CTNNB1
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pShuttle.CC9
Plasmid#192931PurposeGateway compatible vector with BsaI cloning site and Cre-2A-Cas9 expressionDepositorTypeEmpty backboneUseGateway-compatible cloning vectorTagsExpressionMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pNW538_pAAVS1-pAP1(2)-FlpO-ABI-P2A-PYL-FlpO-CAG-U1-frt-STOP-frt-U2-GFP-P2A-luc2-ZeoR
Plasmid#192939PurposeFlpO-based digitizer circuit driven by AP1 promoterDepositorInsertsAP1(2)/minTK promoter
FlpO-ABI
PYL-FlpO
CAG promoter
frt-STOP-frt
GFP
luciferase
pPGK
Zeocin resistance
BFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pGL4.15_pT-REX17
Plasmid#195497PurposeFirefly luciferase promoter expression vector driven only by the promoter element of human lncRNA T-REX17DepositorInsertPromoter region of lncRNA T-REX17
UseTagsluc2-hPESTExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pTFG011_Spike_Parental_D614G_3xFlag
Plasmid#191571PurposeSARS-CoV-2 Spike protein under CMV with D614G, C-term 19 amino acid truncation, and 3x FLAG. For expression and pseudotyping lentiviral particles.DepositorInsertSARS-CoV-2 Spike Protein (S Synthetic)
UseTags3x FLAGExpressionMammalianMutationD614G mutation. Last 19 amino acids truncated.PromoterCMVAvailabilityAcademic Institutions and Nonprofits only
Showing: 3441 - 3460 of 4263 results