-
Plasmid#118706Purposereporter plasmid HIF-responsive promoter (3xHRE) wt- fused to firefly luciferaseDepositorInsertHRE (HIF-responsive promoter (3xHRE)-luc
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo-RL-ZEB2
Plasmid#35536DepositorInsert3'UTR of ZEB2 gene (ZEB2 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceJune 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGL4_10xUAS-MLPmin-luc2
Plasmid#194383PurposeReporter plasmid for split TEV assays; firefly luciferase reporter gene driven by 10x clustered UAS binding sites linked to a minimal MLP promoterDepositorInsert10xUAS-luc2
UseTagsExpressionMammalianMutationPromoterMLP minimalAvailable sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL410_INS421
Plasmid#49057PurposeExpresses luciferase under control of human insulin gene promoterDepositorInserthuman INS promoter (INS Human)
UseTagsExpressionMammalianMutationPromoterINS promoterAvailable sinceOct. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGL4 mIL-17 2kb promoter + CNS-5
Plasmid#20128DepositorInsertmIL-17 (Il17a Mouse)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceJan. 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
-227 hTF (mutant AP-1)
Plasmid#15444DepositorInsertTissue factor promoter (F3 Human)
UseLuciferaseTagsExpressionMammalianMutationBoth AP-1 sites mutatedPromoterAvailable sinceJuly 27, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGL4 mIL-17 2kb RORgamma-t mut
Plasmid#20127DepositorInsertmIL-17 (Il17a Mouse)
UseLuciferaseTagsExpressionMutationRORgamma-t binding site in 0.9kb of 2kb promoter …PromoterAvailable sinceJan. 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSF3-microID
Plasmid#172880Purposeexpresses microID in mammalian cells with the tet-on or tet-off systemDepositorInsertmicroID
UseLuciferase and Retroviral; To be used in a tet-on…TagsmycExpressionMammalianMutationfragment ([aa2-171]) of aquifex aeolicus BirA wit…PromoterTRE-cmvAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL2B-HIF1A prom
Plasmid#40172DepositorInsertHIF1A promoter fragment (HIF1A Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMEH31 Lamp2b HA
Plasmid#168208PurposeMammalian expression of lysosome-associated membrane protein 2b fused to a C-terminal HA tagDepositorInsertLamp2b (LAMP2 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-pGK-Nluc-Fluc-NLS-P2A-H2B-mCherry-IRES-Hygro
Plasmid#115684PurposeNanoLuc-Firefly Luciferase fusion with constitutive nuclear expression for calibration of Nanoluc signal to Firefly Luciferase signal. H2B-mCherry marks chromatin with red fluorescence.DepositorInsertNluc-Fluc-NLS-P2A-H2B-mCherry-IRES-Hygro
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
H1.M11.luc
Plasmid#193707PurposeExpressed a H1-core mutant by introducing multiple 7SK elements (octamer, PSE, spacer, and TATA box)DepositorInsertMutated H1-core promoter with multiple 7SK elements (octamer, PSE, spacer, and TATA box)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
p(A)n-PAUSE
Plasmid#118068PurposeTranscription Blocker - Synthetic polyadenylation signal & RNA polymerase II transcriptional pause signal from the human α2 globin geneDepositorInsertSynthetic polyadenylation signal & RNA polymerase II transcriptional pause signal from the human α2 globin gene
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
proE-cad670-Luc
Plasmid#42083DepositorInsertE-cadherin (CDH1 Human)
UseLuciferaseTagsExpressionMammalianMutationcontains E-cadherin promoter region from -670 to …PromoterE-cadherinAvailable sinceMarch 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
proE-cad178-Luc
Plasmid#42081DepositorInsertE-cadherin (CDH1 Human)
UseLuciferaseTagsExpressionMammalianMutationcontains E-cadherin promoter region from -178 to …PromoterE-cadherinAvailable sinceFeb. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
hGAPDH-rLuc
Plasmid#82479Purposereporter of GAPDH expressionDepositorInsertGAPDH promoter (GAPDH Human)
UseLuciferaseTagsExpressionBacterialMutationPromoterhGAPDHAvailable sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFBD-Akt1(E17K)
Plasmid#86563PurposeExpresses human full-length Akt1(E17K) mutantDepositorInsertAkt1 (AKT1 Human)
UseTags10xHisExpressionInsectMutationE17K mutation in the PH domainPromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
p5xNF-kb_RE
Plasmid#118050PurposeGBPart - Operator/DNA Response element - 5 copies of the NF-kb DNA binding motifDepositorInsert5 copies of the NF-kb DNA binding motif
UseSynthetic Biology; Domestication of dna parts for…TagsExpressionMutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-sbGLuc-ChR2(CS/DA)-EYFP
Plasmid#114106Purposefusion protein of Gaussia luciferase variant slow burn, Channelrhodopsin-2 variant C128S/D156A (stabilized step function opsin), and enhanced yellow fluorescent protein for bioluminescent optogeneticsDepositorInsertsGaussia luciferase, slow burn
Channelrhodopsin 2(CS/DA), stable step function opsin
Enhanced Yellow Fluorescent Protein
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-BCR-ABL-p210-FLAG
Plasmid#205619PurposeExpression of BCR-ABL oncogene in mammalian cellsDepositorInsertBCR-ABL oncogene, p210 isoform b3a2
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2023AvailabilityAcademic Institutions and Nonprofits only