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Plasmid#190034Purposevector for encoding a human codon-optimized High-fidelity RfxCas13d (hfCas13d) driven by CBh promoter, guide RNAs compatible with RfxCas13d driven by hU6, EGFP and mCherry driven by SV40 promotersDepositorInserthumanized hfCas13d
UseTagsExpressionMammalianMutationA134V,A140V,A141V,A143VPromoterCBh, SV40, hU6Available sinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC57-mitoLbNOX
Plasmid#74448PurposeThis plasmid contains human codon optimized sequence of mitoLbNOX which is a water-forming NADH oxidase that can be used to increase NAD+/NADH ratio in mammalian cells.DepositorInsertmitoLbNOX
UseTagsFlag tag with linker and mitochondrial targeting …ExpressionMutationHuman codon optimizedPromoterAvailable sinceJune 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
SPLICS PM-ER Short P2A
Plasmid#164112PurposeDetect the short Plasma membrane-Endoplasmic reticulum contactDepositorInsertSplit YFP PM-ER Short P2A
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3- sgAAVS1.5-CAG-Cas9-T2A-mCherry-P2A-Puro
Plasmid#216246PurposeExpress Cas9 with CAG promoter to improve the expression in hES cells and a highly efficient sgAAVS1.5 for AAVS1 integrationDepositorInsertCas9 and sgAAVS1.5
UseCRISPR; Human safe harbor locus (a avs1) site-spe…TagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
mDC-STAMP-Flag-Myc-CMV26
Plasmid#73583PurposeExpress mouse DC-STAMP in mammalian cellsDepositorInsertDC-STAMP (Dcstamp Mouse)
UseTags3x Flag and c-Myc tagExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRPN265NB-enh-MNase
Plasmid#166035PurposeExpresses a nanobody against GFP fused to micrococcal nuclease for purposes of green Cut & RunDepositorInsertGlutathione-S-Transferase/enhancer/MNase
UseTagsGSTExpressionBacterialMutationPromoterlac promoter / tac promoterAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT/Caggs-NRASV12
Plasmid#20205DepositorInsertNRas (NRAS Human)
UseTransposonTagsExpressionMutationG12VPromoterAvailable sinceFeb. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
pSF-p15ABAD- YFP
Plasmid#186571Purposep15A ori, L-arabinose PBAD promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expressionDepositorInsertYFP
UseTagsExpressionBacterialMutationPromoterBAD promoterAvailable sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCEP4_WT_OCT4
Plasmid#40629DepositorInsertPOU5F1 (POU5F1 Human)
UseTagsExpressionMammalianMutationPromoterEF1-alphaAvailable sinceJuly 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEG BacMam
Plasmid#160451Purposevector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target proteinDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
VEGFR2-mCh
Plasmid#108854PurposeEncodes for human VEGFR2 fluorescently labeled with mCherry on the C-Terminus via a 3 amino acid (GGS) flexible linkerDepositorInsertKDR (KDR Human)
UseTagsLabeled with mCherry on the C-Terminus via a 3 am…ExpressionMammalianMutationPromoterAvailable sinceMay 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
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mEmerald-CD36-C-10
Plasmid#54030PurposeLocalization: Membrane, Excitation: 487, Emission: 509DepositorInsertCD36 (CD36 Human)
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLenti-X1-Neo-GFP-FAM134B-LIRmut
Plasmid#109027Purposestable lentiviral expression of cDNADepositorInsertFAM134B-LIRmut (RETREG1 Human)
UseLentiviralTagseGFPExpressionMammalianMutationDDFELL to DDAEAL aa 453-458 to interrupt FAM134B …PromoterEIF-1a promoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
U6-sgRNA(HEK3)-CMV-SpCas9(D10A)-P2A-EGFP
Plasmid#221233PurposeExpress sgRNA targeting HEK3 loci with nCas9(D10A)DepositorInsertSpCas9(D10A)-P2A-EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEZT-BM
Plasmid#74099PurposeMulti-purpose rapid titer bacmam expression vectorDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseBacmam, baculovirusTagsEGFP following p10 promoterExpressionMammalianMutationPromoterCMV, p10Available sinceMarch 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-copGFP-T2A-Puro
Plasmid#72263PurposeExpress copGFP and puromycin resistance gene under EF1 promoterDepositorInsertcopGFP-T2A-Puro
UseLentiviralTagsExpressionMammalianMutationPromoterelongation factor-1Available sinceJan. 11, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHIV-EGFP.Flag-EZH2-mt2
Plasmid#220245PurposeExpresses an EZH2 mutant for knockout/rescue experiments in mammalian cells.DepositorInsertEZH2 mt 2 (EZH2 Human)
UseLentiviralTagsFlagExpressionMammalianMutationF32A, R34A, D36A, K39A, PRKKKR494-499NAAIRSPromoterEF-1αAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTBL235 NheI-Cas9-KpnI-GSAlinker-FseI-TdT-NotI
Plasmid#126480PurposeExpresses Cas9-GSAlinker-TdT in mammalian cells.DepositorInsertsUseTagsExpressionMammalianMutationChanged codons to reduce repetitive sequences.PromoterCMVAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0103
Plasmid#91000PurposeModule A, Promoter: CmYLCV, Gene: AtCas9, Terminator: HSPDepositorInsertAtCas9
UseCRISPRTagsExpressionMutationPromoterCmYLCVAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only