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Plasmid#191683PurposeFor knocking p2a-tdTomato into LMX1A locusDepositorInsertLMX1A homology arms and p2a-tdTomato
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-NLS-dHgm4Cas13b-3xGFP-NLS
Plasmid#191368PurposeOverexpression of NLS-NLS-dHgm4Cas13b-3xGFP-NLS in human cellsDepositorInsertNLS-NLS-dHgm4Cas13b-3xGFP-NLS
UseTags3xGFPExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
N163 nLV EF-1a-Hp1a(CS)-Frbx2(Frb+FrbWobb)-V5-PGK-Puro
Plasmid#44195DepositorInsertCbx5 (Cbx5 Mouse)
UseLentiviralTagsFrb, Hu Frb x2(WT Frb+codon Wobble Frb), and V5/e…ExpressionMutationN-term chromo domain removedPromoterEF-1aAvailable sinceJune 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
LV-TRE-MyoD-Stop-LacZ PGK-rtTA-IRES-Puro
Plasmid#61441PurposeDox-inducible MyoD with mRNA tag for smFISHDepositorInsertMyoD (Myod1 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
tre-stblHIF: pLenti-TRE-stblHIF-PGK-rtTAA-2A-Puro
Plasmid#177203Purposeencoding stblHIF under inducible promoterDepositorArticleInsertstblHIF
UseLentiviralTagsExpressionMammalianMutationP402A/P577G/N813APromoterTREAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-puro-AS9 (Mito MTS)-Delta N67-mMCL1
Plasmid#45822DepositorInsertAS9(Mito MTS)-Delta N67-mMCL1
UseTagsExpressionMammalianMutationATP Synthase subunit 9 is used as a Mito Targetin…PromoterAvailable sinceJuly 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-NLS-dMisCas13b-3xGFP-NLS
Plasmid#191367PurposeOverexpression of NLS-NLS-dMisCas13b-3xGFP-NLS in human cellsDepositorInsertNLS-NLS-dMisCas13b-3xGFP-NLS
UseTags3xGFPExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCVL TLR 3.1 (Sce target) Ef1a iRFP Puro
Plasmid#50417PurposeFluorescent reporter for DNA cleavage/repair in mammalian cells expressing iRFP with selectable puromycin marker.DepositorInsertseGFP with I-Sce I TS
+3 mCherry
UseLentiviralTagsT2A dislinker and iRFPExpressionMammalianMutationembedded I-Sce I TS from 163-185PromoterSFFVAvailable sinceJan. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
Col7A1-CRISPR-gRNA#exon3-(pSpCas9(BB)-2A-Puro (PX459) V2.0)
Plasmid#124287PurposesgRNA against human Col7A1DepositorInsertCollagen type VII alpha 1 chain (COL7A1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8454 pHR-hU6-LbCpf1 crRNA BB-EF1a-Puro-WPRE
Plasmid#140087PurposeBackbone for cloning U6 driven LbCpf1 (Cas12a) crRNA, with EF1a driven puromycin resistanceDepositorInsertLbCpf1 (LbCas12a) crRNA backbone
UseLentiviralTagsEF1a-PuroExpressionMammalianMutationPromoterU6Available sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
PBRPyCAG-LoxP-ERAS-STOPcassette-Loxp-DsRed-IRES-PURO
Plasmid#52419PurposeConstitutive mammalian expression of ERAS. Upon CRE mediated deletion of ERAS insert, dsRED expression markers is truned on due to concomittant removal of stop cassette.DepositorInsertERAS (ERAS Human)
UseTagsExpressionMutationPromoterCAGGSAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
NFATc3-CRISPR-gRNA#exon3-(pSpCas9(BB)-2A-Puro (PX459) V2.0)
Plasmid#124288PurposesgRNA against human NFATc3DepositorInsertNFATc3 nuclear factor of activated T cells (NFATC3 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
Col7A1-CRISPR-gRNA#exon2-(pSpCas9(BB)-2A-Puro (PX459) V2.0)
Plasmid#124286PurposesgRNA against human Col7A1DepositorInsertCollagen type VII alpha 1 chain (COL7A1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCW-HA-hRb-delta-CDK-T373D-S608D-S612D-puro
Plasmid#212675PurposeExpress tagged hRb phosphosite mutantDepositorInsertRb (RB1 Human)
UseLentiviralTagsHAExpressionMutation15 CDK phospho-sites are mutated to alanines, and…PromoterTREAvailable sinceAug. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro cTnT CCND2-T2A-ChloC-P2A-eGFP
Plasmid#179840Purposedonor plasmid for cardiac-specific CCND2-T2A-Chloc-P2A-eGFP expression in human cellsDepositorInsertCCND2 (CCND2 Human)
UseCRISPR and TALEN; Donor plasmidTagschloride-conducting ChR (ChloC) and eGFPExpressionMammalianMutationPromoterTroponin T promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
EFS-FLAG-SpCas9-6xNLS(c-Myc)-P2A-Puro (pRG214)
Plasmid#221385PurposeLentiviral expression of SpCas9-6xNLS(c-Myc) with puromycin resistance geneDepositorInsertSpCas9
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJune 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-h.Rhno1 Plk1mut7A-C-PAMmut-Myc-DDK-P2A-Puro
Plasmid#211616PurposeRhno1 Plk1mut7ADepositorInserthRHNO1 (RHNO1 Human)
UseTagsmycExpressionMammalianMutationS51A, T52A, S141A, S162A, S163A, S164A, S178APromoterCMVAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-G3635A-R16-Q2L7-T2176C-UGI-Puro
Plasmid#209648PurposeTo install m.G3635 mutation on mtDNADepositorInsertR16-Q2L7-T2176C
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-G3635A-L17-Q2L7-T2176C-UGI-Puro
Plasmid#209646PurposeTo install m.G3635 mutation on mtDNADepositorInsertL17-Q2L7-T2176C
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-h.Rhno1 delta TOPBP1 (T202A)-C-Myc-P2A-Puro
Plasmid#211619PurposeRhno1 delta TOPBP1 (T202A)DepositorInserthRHNO1 (RHNO1 Human)
UseTagsmyc-flagExpressionMammalianMutationT202APromoterCMVAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only