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Plasmid#136097PurposeFP with ER retention signal for C-term fusion with CDS12 with ER targeting signal peptideDepositorInsertCTAG_eGFP(noATG,noStop)-HDEL
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
FKBP-ST*-GFP
Plasmid#163669PurposeExpresses FKBP tagged partial length ST in mammalian cellsDepositorInsertpartial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 (first 113 amino acids) (ST6GAL1 Human)
UseTagsFK506 binding protein (FKBP) and GFPExpressionMammalianMutationThis chimera contains the first 113 amino acids o…PromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
Cavin4b-EGFP
Plasmid#176011PurposeMammalian expression of zebrafish Cavin4b-EGFP. Parton lab clone HXMDepositorInsertCavin4b (cavin4b Zebrafish)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-CT-Tagging
Plasmid#165871PurposeTemplate for amplification of EGFP C-terminal tagging cassetteDepositorInsertVector - CT Tagging
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-RanGAP*
Plasmid#175237PurposeBacterial expression and purification, low affinity SUMOylation substrate, point mutation F562A reduces affinity for E2, increasing the KmDepositorInsertRANGAP1 (RANGAP1 Human)
UseTagsExpressionBacterialMutationAmino acid 562 F to APromotertacAvailable sinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
SIN40C.EFS.eGFP.mArid3a-3'UTR-WT
Plasmid#169299PurposeConstitutive overexpression of the 3'UTR of the murine Arid3a gene after a GFP, to evaluate miRNA binding. Regions not containing miR-125b or let-7c binding regions not included.DepositorInsertArid3a-3'UTR-WT (Arid3a Mouse)
UseLentiviralTagsExpressionMutationPromoterEFSAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-RanGAP
Plasmid#175235PurposeBacterial expression and purification, high affinity SUMOylation substrateDepositorInsertRANGAP1 (RANGAP1 Human)
UseTagsExpressionBacterialMutationPromotertacAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
SIN40C.TRE.dTomato.miR-ctrl.PGK.sfGFP.P2A.Tet3G
Plasmid#169316PurposeDoxycycline-inducible expression of miR-ctrl with dTomato as reporter fluorescent proteinDepositorInsertmiR-ctrl
UseLentiviralTagsdTomatoExpressionMutationPromoterTREAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
SIN40C.SFFV.GFP.miR-ctrl
Plasmid#169305PurposeConstitutive overexpression of miR-ctrl with GFP as a reporter fluorescent proteinDepositorInsertmiR-ctrl
UseLentiviralTagsEGFPExpressionMutationPromoterSFFVAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-NT-Tagging
Plasmid#165868PurposeTemplate for amplification of EGFP N-terminal tagging cassetteDepositorInsertVector - NT Tagging
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP TL1
Plasmid#165834PurposesfGFP green fluorescent protein for N-terminal taggingDepositorInsertEGFP
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP TL5
Plasmid#165835PurposesfGFP green fluorescent protein for C-terminal taggingDepositorInsertEGFP
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAFNF-EGFP
Plasmid#163609PurposeExpresses EGFP Flp-dependently in mammalian cellsDepositorInsertEgfp
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcry:mCherry,-600unc:cct3GFP
Plasmid#172528PurposealphaA-crystallin promoter (cry) drives mCherry expression in the lens. The 600bp unc-45b muscle-specific promoter drives expression of GFP-tagged zebrafish cct3.DepositorInsertcct3-GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
SIN40C.SFFV.KRAB-mArid3a.IRES.GFP
Plasmid#169306PurposeConstitutive overexpression of murine KRAB-Arid3a fusion protein with GFP as reporter fluorescent proteinDepositorInsertKRAB-Arid3a (Arid3a Mouse)
UseLentiviralTagsIRES-EGFPExpressionMutationPromoterSFFVAvailable sinceJuly 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP–acVHH–PB
Plasmid#169685PurposeAn anti-caffeine nanobody responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.DepositorInsertanti-caffeine nanobody
UseTagsmodified STIM1-PB tagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP–COSMO–PB
Plasmid#169684PurposeCOSMO responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.DepositorInsertanti-caffeine nanobody variant
UseTagsmodified STIM1–PB tagExpressionMammalianMutationY104WPromoterCMVAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
SIN40C.SFFV.VP64-mArid3a.IRES.GFP
Plasmid#169313PurposeConstitutive overexpression of murine VP64-Arid3a fusion protein with GFP as reporter fluorescent proteinDepositorInsertVP64-Arid3a (Arid3a Mouse)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
SIN40C.EFS.eGFP.ARID3A-3'UTR-WT
Plasmid#169297PurposeConstitutive overexpression of the 3'UTR of the human ARID3A gene after a GFP, to evaluate miRNA binding. Regions not containing miR-125b or let-7c binding regions not included.DepositorInsertARID3A-3'UTR-WT (ARID3A Human)
UseLentiviralTagsExpressionMutationPromoterEFSAvailable sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
Gfp-rt-vasa
Plasmid#167322PurposePlasmid for synthesis of GFP chimeric RNA that contained vasa-3'UTRs of rainbow trout by in vitro transcription.DepositorInsertrt vasa 3'UTRs (ddx4 rainbow trout)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only