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Plasmid#132407Purposeoverexpression in human cellsDepositorInsertdLwaCas13b
UseLentiviralTagsflagExpressionMammalianMutationR474A; H479A; R1046A; R1051APromoterAvailable sinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
Histone H2B targeted Aurora B FRET sensor Kif2 substrate
Plasmid#45216DepositorInsertHistone H2B-targeted Aurora B FRET sensor
UseTagsExpressionMammalianMutationPromoterT7Available sinceMay 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLentiRNACRISPR_001 - hU6-DR_BsmBI-EFS-PguCas13b-NLS-2A-Puro-WPRE
Plasmid#138143PurposeExpresses PguCas13b in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang cacc. R overhang caacDepositorInsertPguCas13b
UseLentiviralTagsNLSExpressionMutationPromoterAvailable sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-NLS-dPba3Cas13b-3xGFP-NLS
Plasmid#191369PurposeOverexpression of NLS-NLS-dPba3Cas13b-3xGFP-NLS in human cellsDepositorInsertNLS-NLS-dPba3Cas13b-3xGFP-NLS
UseTags3xGFPExpressionMammalianMutationPromoterAvailable sinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJT396 PB ITR and Cp36 attD pEF Puro-2a-tagBFP2 donor
Plasmid#184952PurposeDonor for both PiggyBac and Cp36 RecombinaseDepositorInsertPuroR, P2A, mTagBFP2
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro CAG-CLTX CD32a ITAM CD3z CAR
Plasmid#171965PurposeDonor plasmid for CLTX CD32a-ITAM CD3z CAR expression in human cellsDepositorInsertSynthetic CLTX CD32a ITAM CD3z CAR
UseCRISPR, Synthetic Biology, and TALENTagsExpressionMammalianMutationPromoterCAGAvailable sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
1072 pBabe puroL myr Akt K179M T308A S473A
Plasmid#9015DepositorInsertAkt1 K179M T308A S473A (AKT1 Human)
UseRetroviralTagsHA and MyrExpressionMammalianMutationK179M T308A S473APromoterAvailable sinceNov. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
pGL3-sgSEC61B.N1-CAG-Cas9-T2A-mCherry-P2A-Puro
Plasmid#216251PurposeExpress Cas9 with CAG promoter to improve the expression in hES cells and sgSEC61B.N1 to tag endogenous SEC61B N-terminusDepositorInsertCas9 and sgSEC61B.N1 (SEC61B Human)
UseCRISPRTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
PL752-CLSTN2-LA-p2a-tdTomato-hGHpA-PGK-puroR-CLSTN2-RA
Plasmid#191685PurposeFor knocking p2a-tdTomato into CLSTN2 locusDepositorInsertCLSTN2 homology arms and p2a-tdTomato
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE mNeonGreen-Core (HBc from HBV) IRES puro
Plasmid#122202PurposePlasmid coding for mNeonGreen-Core protein (from HBV AYW) and a puromycin resistance in a lentiviral backbone.DepositorInsertsHBc from HBV AYW
mNeonGreen
UseTagsGGSGGSGGS linkerExpressionMammalianMutationPromoterAvailable sinceApril 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLentiRNACRISPR_002 - hU6-DR_BsmBI-EFS-PguCas13b-NES-2A-Puro-WPRE
Plasmid#138144PurposeExpresses PguCas13b in mammalian cells, localized in the cytoplasm. For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang cacc. R overhang caacDepositorInsertPguCas13b
UseLentiviralTagsNESExpressionMutationPromoterAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dEsCas13d-EGFP-NLS-3xFlag
Plasmid#132410Purposeoverexpression in human cellsDepositorInsertdEsCas13b
UseLentiviralTagsflagExpressionMammalianMutationR295A; H300A; R849A; H854APromoterAvailable sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
LITE2.0 EF1a_TALE(Neurog2)-GS-CIB1(NLS*,∆318-334)_WPRE_hGHpA
Plasmid#47458PurposeLITE2.0 TALE-CIB1. Changes from LITE1.0: glycine-serine linker, mutated endogenous NLS, aa318-334 deletion. This particular TALE targeted to Neurog2. EF1-alpha promoter for ubiquitous mammalian exp.DepositorInsertTALE (Neurog2)-GS-CIB1(NLS*,∆318-334)
UseTagsHAExpressionMammalianMutationPromoterEF1-alphaAvailable sinceOct. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dPspCas13b-2xmNeongreen-NLS-3xFlag
Plasmid#132402Purposeoverexpression in human cellsDepositorInsertdPspCas13b
UseLentiviralTagsflagExpressionMammalianMutationH133A; H1059APromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
HA-HIF2α P405A Δ450-572/Δ820-870-pBabe-Puro
Plasmid#25955DepositorInsertHypoxia inducible factor 2 alpha (EPAS1 Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationcDNA changed amino acids: Proline 405 to Alanin…PromoterAvailable sinceSept. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
HA-HIF2α bHLH* P405A/P531A Δ820-870-pBabe-Puro
Plasmid#25957DepositorInsertHypoxia inducible factor 2 alpha (EPAS1 Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationcDNA changed amino acids: Proline 405 to Alanin…PromoterAvailable sinceSept. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dPspCas13b-mNeongreen-NLS-3xFlag
Plasmid#132401Purposeoverexpression in human cellsDepositorInsertdPspCas13b
UseLentiviralTagsflagExpressionMammalianMutationH133A; H1059APromoterAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dRanCas13b-EGFP-NLS-3xFlag
Plasmid#132409Purposeoverexpression in human cellsDepositorInsertdRanCas13b
UseLentiviralTagsflagExpressionMammalianMutationH147A; H1044APromoterAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dLbaCas13a-EGFP-NLS-3xFlag
Plasmid#132408Purposeoverexpression in human cellsDepositorInsertdLbaCas13b
UseLentiviralTagsflagExpressionMammalianMutationR600A; H605A; R1243A; H1248APromoterAvailable sinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
PL752-OTX2-LA-p2a-EGFPnls-hGHpA-PGK-puroR-OTX2-RA
Plasmid#191690PurposeFor knocking p2a-EGFP into OTX2 locusDepositorInsertOTX2 homology arms and p2a-EGFP
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only