-
Plasmid#144157PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertKDM5C (KDM5C Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SLC38A7_STOP
Plasmid#161260PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC38A7 (SLC38A7 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
JA412
Plasmid#49935PurposeExpresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human KLF4 gene and demethylate CpGs adjacent to the binding siteDepositorInsertTet1CD KL-1 (KLF4 Human)
UseTALENTagsExpressionMutationPromoterEF1aAvailable sinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
SL357
Plasmid#49936PurposeExpresses a TALE-TET1FL (full length TET1) fusion protein engineered to bind a site in the human KLF4 gene and demethylate CpGs adjacent to the binding siteDepositorInsertTet1FL KL-1 (KLF4 Human)
UseTALENTagsExpressionMutationPromoterEF1aAvailable sinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
JA431
Plasmid#49937PurposeExpresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human KLF4 gene and demethylate CpGs adjacent to the binding siteDepositorInsertTet1CD KL-2 (KLF4 Human)
UseTALENTagsExpressionMutationPromoterEF1aAvailable sinceDec. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
TFORF2777
Plasmid#144353PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertKDM5C (KDM5C Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2776
Plasmid#144235PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertKDM5C (KDM5C Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgBap1#2/Cre
Plasmid#173582PurposeExpresses a Bap1-targeting gRNA and Cre-recombinaseDepositorInsertgRNA targeting Bap1 (Bap1 Mouse)
UseCRISPR, Cre/Lox, and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgBap1#1/Cre
Plasmid#173581PurposeExpresses a Bap1-targeting gRNA and Cre-recombinaseDepositorInsertgRNA targeting Bap1 (Bap1 Mouse)
UseCRISPR, Cre/Lox, and LentiviralTagsExpressionMutationPromoterAvailable sinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
EIF2B3-R91H
Plasmid#172080PurposeIn vitro transcription for rescue experimentsDepositorInsertEIF2B3 (EIF2B3 Human)
UseTagsExpressionMammalianMutationc.272G>APromoterCMVAvailable sinceJuly 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
FGR gRNA (BRDN0001146726)
Plasmid#75719Purpose3rd generation lentiviral gRNA plasmid targeting human FGRDepositorInsertFGR (FGR Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
FGR gRNA (BRDN0001147671)
Plasmid#75720Purpose3rd generation lentiviral gRNA plasmid targeting human FGRDepositorInsertFGR (FGR Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
FGR gRNA (BRDN0001147903)
Plasmid#75721Purpose3rd generation lentiviral gRNA plasmid targeting human FGRDepositorInsertFGR (FGR Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFP.E222
Plasmid#138248PurposeThree input / three output logic circuit plasmid - Expresses different intein split ECFs from different inducible promotersDepositorInsertsaraC (araC )
ECF20.N.(VDA).M86.N2
Terminator
SspGyrB.C2.(SAK).ECF16.C
Terminator
rhaS
ECF17.N.(NPC).NrdJ-1.N2
Terminator
M86.C2.(SDL).ECF20.C
Terminator
luxR
Terminator
ECF16.N.(AGG).SspGyrB.N2
Terminator
NrdJ-1.C2.(SEI).ECF17.C
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23115, P(araBAD), P(lux2), P(rhaBAD), iJ23101, a…Available sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-BTLA-Fc(DAPA)-AviTag-6xHis
Plasmid#156513PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertBTLA (BTLA Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSin-EF2-DMD53-GFP
Plasmid#138353PurposeExpressing a GFP reporter cassette, whose GFP reading frame is disrupted by insertion of partial DMD exon 53 sequencesDepositorInsertDMD exon 53 partial sequence to disrupt GFP reading frame (DMD Human)
UseCRISPR and LentiviralTagsNoExpressionMammalianMutationPromoterEF2Available sinceNov. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SynI-CreOn-FlpOff-Kir2.1MUT-P2A-EGFP
Plasmid#176279PurposeViral vector for co-expression of non-functional Kir2.1 and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.DepositorInsertKir2.1MUT-P2A-EGFP (Kcnj2 Mouse, Synthetic)
UseAAV and Cre/LoxTagsMycExpressionMammalianMutationGYG to AAA (aa144-146)Promoterhuman Synapsin IAvailable sinceJan. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-BTLA-COMP5AP-AviTag-9xHis
Plasmid#157078PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertBTLA (BTLA Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_GPR137B_p.C212S
Plasmid#82757PurposeGateway Donor vector containing GPR137B, part of the Target Accelerator Plasmid Collection.DepositorInsertGPR137B (GPR137B Human)
UseGateway entry vectorTagsExpressionMutationC212SPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_GPR137B_p.Y135N
Plasmid#82752PurposeGateway Donor vector containing GPR137B, part of the Target Accelerator Plasmid Collection.DepositorInsertGPR137B (GPR137B Human)
UseGateway entry vectorTagsExpressionMutationY135NPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only