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Plasmid#55196PurposeExpresses Csy4 in Mammalian cells for site-specific RNA processingDepositorInsertCsy4/Cas6
UseSynthetic BiologyTags6xHISExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
DHB-mVenus
Plasmid#136461PurposeFluorescently tagged segment of DNA Helicase B that translocates from the nucleus to the cytoplasm in response to phosphorylation by CDK2DepositorInsertDNA Helicase B (HELB Human)
UseLentiviralTagsmVenusExpressionMutationPromoterAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
PresentER-SIINFEKL (mCherry)
Plasmid#102945PurposePresentER minigene encoding H2-Kb ligand SIINFEKL (mCherry)DepositorInsertSIINFEKL (OVAL Chicken, Synthetic)
UseRetroviralTagsExpressionMammalianMutationOVA257-264PromoterLTRAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2pACryGFP
Plasmid#64022PurposeGateway destination vector with zebrafish aA-crystallin promoter driving GFP in lensDepositorInsertGFP
UseGatewayTagsExpressionMutationPromoteralpha-Acrystallin promoterAvailable sinceMay 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40
Plasmid#105540PurposeAAV expression of EGFP-Cre from hSyn promoterDepositorHas ServiceAAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9InsertEGFP-Cre
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterhSynAvailable sinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV CMV-dSaCas9-KRAB-bGHpA
Plasmid#106219PurposeAAV expressing deactivated S. aureus Cas9 fused to a KRAB repressorDepositorInsertdead S. aureus Cas9 KRAB
UseAAV and CRISPRTagsHA-NLSExpressionMammalianMutationD10A and N580APromoterCMVAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
T4 Lysozyme WT
Plasmid#18110DepositorInsertT4 Lysozyme (e Bacteriophage T4)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 26, 2008AvailabilityAcademic Institutions and Nonprofits only -
pVE13300
Plasmid#137715PurposeExpresses impLbCas12a in mammalian cells.DepositorInsertimpLbCas12a
UseTags3xHA, NLS, and SV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-CSNK1A1
Plasmid#23355DepositorInsertCSNK1A1 (CSNK1A1 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pRCC-K
Plasmid#81191PurposeExpression of Cas9 and gRNA cassette in S. cerevisiae; Kan ResistanceDepositorInsertsCas9
empty gRNA cassette
UseCRISPRTagsNTSExpressionYeastMutationPlease see depositor comments below. and WT - cod…PromoterROX3 and SNP52pAvailable sinceSept. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBABE HA Chk2 WT
Plasmid#41901DepositorInsertCHK2 (CHEK2 Human)
UseRetroviralTagsHAExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
VSV.G
Plasmid#14888DepositorInsertVSV-G Envelope
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV-flex-taCasp3-TEVp
Plasmid#45580DepositorUseAAV; Adeno associated viral vectorTagsExpressionMutationLinker replaced with a TEV protease cleavage sitePromoterEF1aAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1 CMYC
Plasmid#164145PurposeDoxycycline inducible expression of c-MYCDepositorInsertMYC (MYC Human)
UseLentiviralTagsExpressionMammalianMutationPromoterDoxycycline inducible promoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti HRE-Luc pGK Hygro
Plasmid#118706Purposereporter plasmid HIF-responsive promoter (3xHRE) wt- fused to firefly luciferaseDepositorInsertHRE (HIF-responsive promoter (3xHRE)-luc
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV5B-TGFbeta receptor I K232R
Plasmid#11763DepositorInsertTGFbeta receptor I (TGFBR1 Human)
UseTags6xHISExpressionMammalianMutationK232R: kinase deadPromoterCMVAvailable sinceMay 12, 2006AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBAD-I-SceI
Plasmid#60960PurposePlasmid that allows an arabinose-inducible expression of the I-SceI homing endonuclease - for the en passant (two-step Red-mediated) mutagenesisDepositorInsertI-SceI restriction enzyme
UseTagsExpressionMutationPromoterAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pgRNA-humanized
Plasmid#44248PurposeExpression of customizable guide RNA (gRNA) under cotnrol of murine U6 promoter, also contains a CMV-puro-t2A-mCherry expression cassette for mammalian gene knockdownDepositorInsertguide RNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermouse U6Available sinceApril 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB_ATP1.0-IRES-mCherry-CAAX
Plasmid#167582PurposeExpress the ATP sensor GRAB_ATP1.0 in mammalian cellsDepositorInsertATP sensor GRAB_ATP1.0 (P2RY1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only