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px330-mcherry

Plasmid

#98750

Purpose

Cas9 from S.pyogenes with CMV-mcherry cassette, and cloning backbone for sgRNA

Depositor

Jinsong Li

Article

Wu et al Cell Stem Cell. 2013 Dec 5;13(6):659-62.

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-DsRed_IRES_EGFP

Plasmid

#92194

Purpose

Lentiviral overexpression vector to make stable bicistronic cell line for control (EGFP) screen

Depositor

Huda Zoghbi

Article

Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

Insert

DsRed-IRES-EGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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pHM129

Plasmid

#225534

Purpose

Encodes destablized mCherry under AR8 promoter, and constitutive BFP

Depositor

Jared Toettcher

Article

McNamara et al Nat Cell Biol. 2024 Oct 2. doi: 10.1038/

Inserts

mCherry
BFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pJFRC7-20XUAS-IVS-mCD8::GFP

Plasmid

#26220

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Aug 9. ():.

Insert

mCD8::GFP

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 19, 2010

Availability

Academic Institutions and Nonprofits only

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pHelp

Plasmid

#227583

Purpose

Accessory plasmid with a sgRNA cassette for Cas9, and ampicillin resistance

Depositor

Chengzhang Fu

Article

Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

Insert

guide RNA scaffold

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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L304-EGFP-Tubulin-WT

Plasmid

#64060

Purpose

EGFP fused at the N-terminus of tubulin; lentiviral construct

Depositor

Weiping Han

Article

Yang et al Biochem J. 2013 Feb 1;449(3):605-12. doi

Insert

tubulin

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only

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pZac2.1-GfaABC1D-mCherry-hPMCA2w/b

Plasmid

#111568

Purpose

Expresses a plasma membrane calcium pump hPMCA2w/b

Depositor

Baljit Khakh

Article

Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

Has Service

AAV5

Insert

Plasma membrane calcium pump 2, variant w/b (ATP2B2 Human)

Use

AAV

Tags

mCherry

Expression

Mutation

Promoter

Available since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pCW-Cas9-2A-EGFP

Plasmid

#167928

Purpose

Doxycycline-inducible Cas9 expression tracked by EGFP fluorescence marker.

Depositor

Ronald Germain

Article

Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

Insert

T2A-EGFP

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

tTRE, hPGK

Available since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-syn-jGCaMP8s-WPRE

Plasmid

#162374

Purpose

AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Has Service

AAV Retrograde, AAV1, AAV5, and AAV9

Insert

jGCaMP8s

Use

AAV

Tags

6xHis

Expression

Mammalian

Mutation

S26M F286Y Q315H

Promoter

Synapsin

Available since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti Lifeact-EGFP BlastR

Plasmid

#84383

Purpose

Lentiviral expression of EGFP-tagged Lifeact with Blasticidin selection in cells including neurons

Depositor

Ghassan Mouneimne

Article

Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

Insert

Lifeact

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

CMV immediate early

Available since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pMSCV-Blasticidin

Plasmid

#75085

Purpose

retrovirus-mediated gene transfer into mammalian cells

Depositor

David Mu

Article

Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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pRN111

Plasmid

#84463

Purpose

pJB38-NWMN29-30 + SarA_P1-mCherry-Term

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

SarA_P1-mCherry from pRN11

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pT3-EF1A-MYC-IRES-luc

Plasmid

#129775

Purpose

Transposon based vector that expresses human MYC followed by IRES and firefly luciferase

Depositor

Amaia Lujambio

Article

Ruiz de Galarreta et al Cancer Discov. 2019 Jun 11. pii: 2159-82

Insert

human MYC (MYC Human)

Use

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

EF1A

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pXR004: CasRx pre-gRNA cloning backbone

Plasmid

#109054

Purpose

hU6-driven expression of guide RNAs compatible with CasRx. Contains BbsI sites for guide cloning flanked by 5' and 3' full-length DRs

Depositor

Patrick Hsu

Article

Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pH2B-miRFP703

Plasmid

#80001

Purpose

Histone 2B labeling with near-infrared fluorescent protein miRFP703

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

histone cluster 1 H2B family member j (H2BC11 Human)

Use

Tags

miRFP703

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pHLSec2-FN-YPet

Plasmid

#65421

Purpose

Expresses in mammalian cells human plasma fibronectin with YPet inserted between FNIII domains 6 and 7

Depositor

Harold Erickson

Article

Ohashi et al J Biol Chem. 2011 Nov 11;286(45):39188-9

Insert

human plasma fibronectin with YPet inserted between FNIII domains 6 and 7 (FN1 Human)

Use

Tags

YPet

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG2-EGFP-C-IFT144

Plasmid

#218740

Purpose

Expresses C-terminally EGFP-tagged IFT144 in mammalian cells

Depositor

Kazuhisa Nakayama

Insert

IFT144 (WDR19 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG2-EGFP-C-IFT139

Plasmid

#218738

Purpose

Expresses C-terminally EGFP-tagged IFT139 in mammalian cells

Depositor

Kazuhisa Nakayama

Insert

IFT139 (TTC21B Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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PB6K-mcherry-iATPsnFR1.1

Plasmid

#227239

Purpose

Inducible ATP sensor for Pseudomonas putida

Depositor

Fuzhong Zhang

Article

Mu et al Nat Commun. 2024 Jun 21;15(1):5299. doi:

Insert

iATPsnFR1.1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-OSKM

Plasmid

#20321

Purpose

Lentiviral plasmid for tet-inducible expression of mouse Oct4, Sox2, Klf4 and Myc for iPS cell generation

Depositor

Rudolf Jaenisch

Article

Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

Oct-4 (Pou5f1, Sox2, Klf4, Myc Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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