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pENTR1a-jAspSnFR3-mRuby3-NLS

Plasmid

#203489

Purpose

Nuclear directed jAspSnFR3-mRuby3 in Gateway cloning entry vector

Depositor

Lucas Sullivan

Article

Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

Insert

jAspSnFR3-mRuby3-NLS

Use

Gateway entry vector

Tags

Nuclear localization signal and mRuby3

Expression

Mutation

Promoter

Available since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR1a-MTS-jAspSnFR3-mRuby3

Plasmid

#203490

Purpose

Mitochondrial directed jAspSnFR3-mRuby3 in Gateway cloning entry vector

Depositor

Lucas Sullivan

Article

Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

Insert

MTS-jAspSnFR3

Use

Gateway entry vector

Tags

His-tag, Mitochondrial localization signal, and m…

Expression

Mutation

Promoter

Available since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pLX304-CAG-jAspSnFR3-mRuby3

Plasmid

#203500

Purpose

jAspSnFR3-mRuby3 in lentiviral expression vector

Depositor

Lucas Sullivan

Article

Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

Insert

jAspSnFR3-mRuby3

Use

Lentiviral

Tags

His-tag and mRuby3

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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PB-Blast-Dest-LifeAct-mRuby

Plasmid

#194907

Purpose

To generate LifeAct-mRuby expressing stable cell lines with piggybac transposase co-expression and blastocidin selections.

Depositor

Clare Buckley

Article

Liang et al EMBO J. 2022 Aug 22:e111021. doi: 10.152

Insert

LifeAct

Use

Mouse Targeting

Tags

mRuby

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pBS-TRE-mRuby3-WPRE

Plasmid

#193336

Purpose

Expresses mRuby3 under the TRE promoter in mammalian cells

Depositor

Takeshi Imai

Article

Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

Insert

mRuby3

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2-CIB1-mRuby-Rab35

Plasmid

#194334

Purpose

For use in light-induced Rab35 protein inactivation through the interaction with CRY2

Depositor

Heidi Hehnly

Article

Aljiboury et al PLoS Genet. 2023 May 15;19(5):e1010765.

Inserts

CIB1 (CIB1 Mustard Weed)
Rab35 (RAB35 Human)

Use

Tags

mRuby

Expression

Mammalian

Mutation

Promoter

CMV IE94

Available since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pTS2395-Tier1-PhCMV-Igk_mRuby2_KDEL

Plasmid

#169533

Purpose

Tier-1 vector encoding PhCMV-driven red fluorescent protein mRuby2 localized to the ER (PhCMV-Igk-mRuby2-KDEL-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven ER-localized monomeric red fluorescent protein mRuby2

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2389-Tier1-PhCMV-p2a_mRuby

Plasmid

#169526

Purpose

Tier-1 vector encoding PhCMV-driven red fluorescent protein mRuby2 fused to a "self-cleaving" peptide p2a (PhCMV-p2a_mRuby2-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven P2A-fused enhanced yellow fluorescent protein

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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p23-mRuby3-DDX21 HGV

Plasmid

#179233

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Wu et al Science. 2021 Jul 30;373(6554):547-555.

Insert

DDX21 (DDX21 Human)

Use

Tags

mRuby3

Expression

Mammalian

Mutation

D338H, E339G

Promoter

EF-1alpha

Available since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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acta1-mRuby2-VHH[GFP]

Plasmid

#163856

Purpose

Zebrafish muscle specific expression of mRuby2-VHH[GFP]. Parton lab clone FRX

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

VHH[GFP]

Use

In vitro transcription of rna

Tags

Ruby2

Expression

Mutation

Promoter

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR CMVie-Lifeact-mRuby2 L1-R5

Plasmid

#84389

Purpose

Multisite Gateway entry vector for expression of mRuby2-tagged Lifeact in cells including neurons

Depositor

Ghassan Mouneimne

Article

Parker et al eNeuro. 2018 Sep 27;5(5). pii: eN-MNT-01

Insert

Lifeact

Use

Gateway

Tags

mRuby2

Expression

Mammalian

Mutation

Promoter

CMV immediate early

Available since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

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PB-CAG-mRuby3-Gal8-P2A-Zeo

Plasmid

#150815

Purpose

Mammalian expression of mRuby3-Galectin8 (Gal8) fusion protein

Depositor

Jordan Green

Article

Rui et al Sci Adv. 2022 Jan 7;8(1):eabk2855. doi:

Insert

mRuby3-Gal8-P2A-Zeo (LGALS8 Human, Synthetic)

Use

Tags

Gal8 is fused to the c-terminus of mRuby3

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-fDIO-mRuby3-2A-Cre

Plasmid

#203844

Purpose

Flp-dependent Cre and mRuby3 expression

Depositor

Ivo Spiegel

Article

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Insert

Cre

Use

AAV

Tags

mRuby3-2A

Expression

Mutation

Promoter

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-fDIO-mRuby3-2A-dCre

Plasmid

#203845

Purpose

Flp-dependent enzymatically inactive Cre and mRuby3 expression

Depositor

Ivo Spiegel

Article

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Insert

dCre

Use

AAV

Tags

mRuby3-2A

Expression

Mutation

Enzymatically inactive Cre

Promoter

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-MPAct-mRuby3-IRES-Puro

Plasmid

#155231

Purpose

lentiviral MPAct construct

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

Lentiviral expression of MPAct-mRuby sensor

Use

Lentiviral

Tags

mRuby3

Expression

Mutation

Promoter

Available since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-hSyn1-mRuby2-T2A-GCaMP6f

Plasmid

#197595

Purpose

Lentiviral transfer vector for the neuronal expression of mRuby2-T2A-GCaMP6f (fluorescent reporter for Calcium imaging)

Depositor

Patrik Verstreken

Insert

mRuby2-T2A-GCaMP6f

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hSynI

Available since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-NLS-dPspCas13b-3xmRuby3-NLS

Plasmid

#132405

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPspCas13b

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

H133A; H1059A

Promoter

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pFSynW Syt1 I367T IRES mRuby3

Plasmid

#170642

Purpose

Expression of syt1 in cultured neurons by lentiviral transduction

Depositor

Edwin Chapman

Article

Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

Insert

SYT1 (Syt1 Rat)

Use

Lentiviral

Tags

Expression

Mutation

I367T

Promoter

Available since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-CDH5-mRuby3-IRES-puro

Plasmid

#85144

Purpose

lentiviral expression of human VE-cadherin, C-terminally tagged with mRuby3

Depositor

Tobias Meyer

Article

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Insert

CDH5-mRuby3 (VE-cadherin, C-terminally tagged with mRuby3 (CDH5 Human)

Use

Lentiviral

Tags

mRuby3

Expression

Mutation

Promoter

CAG

Available since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pHAGE-NLS-dPguCas13b-3xmRuby3-NLS

Plasmid

#132406

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPguCas13b

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

H151A; H1121A

Promoter

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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