Addgene

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pHR-PGK-ABI-tagBFP-dCas9

Plasmid

#169446

Purpose

expression of AB1-fused-dcas9 plasmids

Depositor

Stanley Qi

Article

Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

Insert

ABI-tagBFP-dCas9

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pCDH-CAG-NCreIntN-T2A-mTagBFP2

Plasmid

#160500

Purpose

Expresses NCreIntN in mammalian cells in split intein-mediated split-Cre recombinase applications

Depositor

Shawn Je

Article

Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

Insert

NCreIntN

Use

Cre/Lox and Lentiviral

Tags

mTagBFP2

Expression

Mammalian

Mutation

Promoter

CAG

Availability

Academic Institutions and Nonprofits only

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pCDH-hSyn-NCreIntN-T2A-mTagBFP2

Plasmid

#160502

Purpose

Expresses NCreIntN in neurons in split intein-mediated split-Cre recombinase applications

Depositor

Shawn Je

Article

Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

Insert

NCreIntN

Use

Cre/Lox and Lentiviral

Tags

mTagBFP2

Expression

Mammalian

Mutation

Promoter

hSyn

Availability

Academic Institutions and Nonprofits only

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pCDH-CAG-NCreIntN-EF1a-mTagBFP2

Plasmid

#160507

Purpose

Expresses NCreIntN in mammalian cells in split intein-mediated split-Cre recombinase applications

Depositor

Shawn Je

Article

Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

Insert

NCreIntN

Use

Cre/Lox and Lentiviral

Tags

mTagBFP2

Expression

Mammalian

Mutation

Promoter

CAG

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-mtagBFP-2A RIGI K270A

Plasmid

#167290

Purpose

Lentiviral expression construct encoding a TagBFP in frame with a self-cleaving catalytically dead (K270A) RLR sensor RIG-I

Depositor

Agnel Sfeir

Article

Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

Insert

DDX58 (RIGI Human)

Use

Lentiviral

Tags

tagBFP

Expression

Mammalian

Mutation

K270A

Promoter

SFFV

Availability

Academic Institutions and Nonprofits only

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pTol2-nbt-mTagBFP2-P2A-mScarlet

Plasmid

#196115

Purpose

zebrafish expression of nbt-mTagBFP2-P2A-mScarlet

Depositor

Reinhard Köster

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

nbt-mTagBFP2-P2A-mScarlet

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pMMK ENTR 4 CMV GST mTagBFP

Plasmid

#206259

Purpose

ENTR Vector 4 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes mTagBFP localised to Golgi organelles under the control of CMV promoter.

Depositor

Imre Berger

Article

Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

Insert

GTS mTagBFP

Use

Multimate/gateway entr 4

Tags

BFGT1 (Golgi localisation peptide)

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1a-mTagBFP-T2A-TetOn3G

Plasmid

#179888

Purpose

Expresses TetOn3G transcription factor selectable by hygromycin resistance (Lentiviral vector)

Depositor

Patrick Caswell

Article

Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

Insert

TetOn3G

Use

Lentiviral

Tags

mTagBFP

Expression

Bacterial and Mammalian

Mutation

Promoter

EF1a

Availability

Academic Institutions and Nonprofits only

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pCMV-PB1-AG-FRB-TEVcs-mTagBFP2-HA

Plasmid

#178868

Purpose

Mammalian expression of a protein of interest fused to the C-terminus of PB1-AzamiGreen-FRB-TEVcs-mTagBFP-HA

Depositor

Shinya Tsukiji

Article

Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

Insert

PB1-AzamiGreen-FRB-TEVcs(ENLYFQL)-mTagBFP2-HA

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pCMV-PB1-AG-FRB-TEVuncs-mTagBFP2-HA

Plasmid

#178869

Purpose

Mammalian expression of a protein of interest fused to the C-terminus of PB1-AzamiGreen-FRB-TEVuncs-mTagBFP-HA

Depositor

Shinya Tsukiji

Article

Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

Insert

PB1-AzamiGreen-FRB-TEVuncs(NEYLQFL)-mTagBFP2-HA

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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Fucci(CA) hCdt1-iRFP hGeminin-TagBFP2

Plasmid

#190181

Purpose

Fluorescent reporter vector to visualize all of cell cycle phases.

Depositor

Toshiro Sato

Article

Ohta et al Nature. 2022 Jul 7. pii: 10.1038/s41586-

Inserts

iRFP
hCdt1(1/100)Cy(-) (Cdt1 Synthetic)
TagBFP2
hGeminin(1/110)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pSA_0_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2-T2A-sGFP1-10

Plasmid

#199487

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Article

Miles et al bioRxiv 2023

Insert

Gal4vp16/4xnrUAS-mTagBFP2-T2A-sGFP1-10

Use

CRISPR

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pSA_1_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2-T2A-sGFP1-10

Plasmid

#199488

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Article

Miles et al bioRxiv 2023

Insert

Gal4vp16/4xnrUAS-mTagBFP2-T2A-sGFP1-10

Use

CRISPR

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pSA_2_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2-T2A-sGFP1-10

Plasmid

#199489

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Article

Miles et al bioRxiv 2023

Insert

Gal4vp16/4xnrUAS-mTagBFP2-T2A-sGFP1-10

Use

CRISPR

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pSA_0_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2-T2A-sGFP11x7

Plasmid

#199490

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Article

Miles et al bioRxiv 2023

Insert

Gal4vp16/4xnrUAS-mTagBFP2-T2A-sGFP11x7

Use

CRISPR

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pSA_1_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2-T2A-sGFP11x7

Plasmid

#199491

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Article

Miles et al bioRxiv 2023

Insert

Gal4vp16/4xnrUAS-mTagBFP2-T2A-sGFP11x7

Use

CRISPR

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pSA_2_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2-T2A-sGFP11x7

Plasmid

#199492

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Article

Miles et al bioRxiv 2023

Insert

Gal4vp16/4xnrUAS-mTagBFP2-T2A-sGFP11x7

Use

CRISPR

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pTP341 pHAGE2 CMV TagBFP-3xFLAG

Plasmid

#199391

Purpose

Lentiviral transfer plasmid for constitutive expression of a TagBFP-3xFLAG in mammalian cells

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Insert

TagBFP-3xFLAG

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pCA27-pHR-MiniCMV-TriTag (mTagBFP)

Plasmid

#199458

Purpose

Expression of TriTag (mTagBFP) in mammalian cells

Depositor

Baohui Chen

Article

Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

Insert

TriTag

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

MiniCMV

Availability

Academic Institutions and Nonprofits only

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PB-CAG-DD-3xFLAG-hSOX17-GS-TagBFP-BGHpA

Plasmid

#172226

Purpose

Expression vector with Shield-1 stabilizable hSOX17 fused to TagBFP.

Depositor

Alexander Meissner

Article

Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

Insert

SOX17 (SOX17 Human)

Use

Piggybac transposon

Tags

3XFLAG, DD-domain, GS-linker, and TagBFP

Expression

Mammalian

Mutation

Promoter

CAG

Availability

Academic Institutions and Nonprofits only

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