Addgene

We narrowed to 2,242 results for: PRK

Showing: 321 - 340 of 2242 results

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pRK5-LAMP1-FLAG

Plasmid

#71868

Purpose

overexpression

Depositor

David Sabatini

Article

Wang et al Science. 2015 Jan 9;347(6218):188-94. do

Insert

LAMP1 (LAMP1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

cmv

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pRK-Myc-Smurf2

Plasmid

#13678

Depositor

Ying Zhang

Article

Zhang et al Proc Natl Acad Sci U S A. 2001 Jan 30. 9

Insert

Smurf2 (SMURF2 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only
pRK5 HA-RagC(Q120L)

Plasmid

#99720

Purpose

Mammalian expression of HA-RagC(Q120L)

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Insert

RagC (RRAGC )

Use

Tags

HA

Expression

Mutation

Q120L

Promoter

CMV

Available since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5 BARK1 minigene

Plasmid

#14695

Depositor

Robert Lefkowitz

Article

Koch et al J Biol Chem. 1994 Feb 25. 269(8):6193-7.

Insert

BARK1 minigene (GRK2 Bovine)

Use

Tags

3'UTR of human b-globin

Expression

Mammalian

Mutation

BARK1 amino acids 495-689

Promoter

Available since

June 19, 2007

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-K6R

Plasmid

#121153

Purpose

Mammalian expression of HA tagged ubiquitin K6R

Depositor

Josef Kittler

Article

Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

Insert

Ubiquitin C (UBC Human)

Use

Tags

Expression

Mammalian

Mutation

K6 mutated to an R

Promoter

Available since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5-myc-Rac1-wt

Plasmid

#12985

Depositor

Gary Bokoch

Insert

Rac1 (RAC1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

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pRK5 CASTOR1-HA Cterm

Plasmid

#84487

Purpose

overexpression of CASTOR1 with HA tag

Depositor

David Sabatini

Article

Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

Insert

CASTOR1 (GATSL3 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only
pX462-hPRKAA1-gRNA_B

Plasmid

#74375

Purpose

gRNA_B to knockout human AMPK alpha 1 using Cas9n

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

human AMPK alpha 1 exon1 gRNA (PRKAA1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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pRK5 HA GST rap2a

Plasmid

#14952

Depositor

David Sabatini

Article

Sancak et al Mol Cell. 2007 Mar 23. 25(6):903-15.

Insert

rap2a (RAP2A Human)

Use

Tags

HA-GST

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2007

Availability

Academic Institutions and Nonprofits only
pRK5-myc-RhoA-wt

Plasmid

#12962

Depositor

Gary Bokoch

Insert

RhoA (RHOA Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only
pRK5-FLAG-C12orf66

Plasmid

#87047

Purpose

mammalian expression of C12orf66

Depositor

David Sabatini

Article

Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

Insert

C12orf66 (C12orf66 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-ITFG2

Plasmid

#87044

Purpose

mammalian expression of ITFG2

Depositor

David Sabatini

Article

Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

Insert

ITFG2 (ITFG2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5-mFzd5-1D4

Plasmid

#42267

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Frizzled 5 (Fzd5 Mouse)

Use

Tags

1D4

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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pRK5-HA GST Rheb1

Plasmid

#19310

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

RHEB Ras homolog enriched in brain (RHEB Human)

Use

Tags

GST and HA

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only
pX462-hPRKAA1-gRNA_A

Plasmid

#74374

Purpose

gRNA_A to knockout human AMPK alpha 1 using Cas9n

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

human AMPK alpha 1 exon1 gRNA (PRKAA1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

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pFLAG-Cherry-N1-PRKAG1

Plasmid

#30308

Depositor

Jay Brenman

Article

Kazgan et al Mol Biol Cell. 2010 Oct 1. 21(19):3433-4

Insert

AMP-activated Protein Kinase γ1 (PRKAA1 Human)

Use

Tags

FLAG and mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 23, 2011

Availability

Academic Institutions and Nonprofits only
pRK5 HA GST Rheb1

Plasmid

#14951

Depositor

David Sabatini

Article

Sancak et al Mol Cell. 2007 Mar 23. 25(6):903-15.

Insert

Rheb1 (RHEB Human)

Use

Tags

HA-GST

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2007

Availability

Academic Institutions and Nonprofits only

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Flag-Daxx/pRK5

Plasmid

#27974

Depositor

Xiaolu Yang

Article

Tang et al Nat Cell Biol. 2006 Aug . 8(8):855-62.

Insert

Daxx (DAXX Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 23, 2011

Availability

Academic Institutions and Nonprofits only
pRK5 Myc-RIP1

Plasmid

#44159

Depositor

Xin Lin

Article

Blonska et al Mol Cell Biol. 2004 Dec;24(24):10757-65.

Insert

RIP1 (RIPK1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

April 18, 2013

Availability

Academic Institutions and Nonprofits only
Cterminal HA WDR59 pRK5

Plasmid

#46328

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

WDR59 (WDR59 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

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