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GFP-ataxin-3 C14A

Plasmid

#89977

Purpose

Expresses catalytic inactive GFP-tagged ataxin-3

Depositor

Nico Dantuma

Article

Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

Insert

ataxin-3 (ATXN3 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

changed Cysteine 14 to Alanine

Promoter

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-ataxin-3 *SIM

Plasmid

#89979

Purpose

Expresses GFP-tagged ataxin-3 with a mutation in the SUMO-interacting motif

Depositor

Nico Dantuma

Article

Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

Insert

ataxin-3 (ATXN3 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

mutated 162IFVV to 162AFAA

Promoter

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-ataxin-3 *VBM

Plasmid

#89976

Purpose

Expresses GFP-tagged ataxin-3 with a mutation in the VCP-binding motif

Depositor

Nico Dantuma

Article

Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

Insert

ataxin-3 (ATXN3 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

mutated 282RKRR to 282HNHH

Promoter

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pCT-CMV-UBE3B(C1036A)-copGFP-Puro

Plasmid

#107344

Purpose

Expresses UBE3B mutant C1036A fused to copGFP in mammalian cells.

Depositor

Robert Sobol

Article

Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

Insert

UBE3B(C1036A)copGFP (UBE3B Human)

Use

Lentiviral

Tags

copGFP

Expression

Mammalian

Mutation

mutation C1036A

Promoter

CMV

Available since

April 19, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3-eGFP-NLS-RNF168

Plasmid

#133977

Purpose

mammalian expression vector of eGFP tagged wild type RNF168

Depositor

Daniel Durocher

Article

Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

Insert

RNF168 (RNF168 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

siRNA resistant sequence: GAGGAGTCGTGTTTATTGA

Promoter

CMV

Available since

Nov. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDestTol2 ZfUbi::cMyc-oDam_f_GFPoNLS

Plasmid

#85821

Purpose

iDamID plasmid. To stably and ubiquitously express the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

Depositor

Joachim Wittbrodt

Article

Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

Insert

Zf Ubi::cMyc oDam_f_GFPoNLS

Use

Tol2 based vector

Tags

cMyc N terminal to oDam_f_GFPoNLS and oNLS (optim…

Expression

Mutation

L122A. Codon optimization compatible to work in M…

Promoter

Available since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-PARP1

Plasmid

#211578

Purpose

CMV driven PARP1-eGFP expressing construct with IRES-Neomycin selection

Depositor

Chris Lord

Article

Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

Insert

PARP1 (PARP1 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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PB-PARP1–eGFP

Plasmid

#211552

Purpose

A piggyBac vector containing CMV-PARP1-eGFP-IRES-Neo cassette.

Depositor

Chris Lord

Article

Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

Insert

PARP1 (PARP1 Human)

Use

Piggybac

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-BSD-tet-mEGFP-MIB1

Plasmid

#140241

Purpose

Lentiviral vector expressing mEGFP-tagged MIB1 upon tetracycline treatment

Depositor

Urs Greber

Article

Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

Insert

Tet-inducible mEGFP-MIB1 (MIB1 Human)

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

TRE

Available since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-PARP1-E988K

Plasmid

#211580

Purpose

CMV driven PARP1-E988K -eGFP expressing construct with IRES-Neomycin selection

Depositor

Chris Lord

Article

Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

Insert

PARP1 (PARP1 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Contains E988K mutation

Promoter

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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