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Plasmid#79497PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) Human GLI1 CDSDepositorInsertGLI1 (GLI1 Human)
UseMultisite gateway entry cloneTagsExpressionMutationPromoterno PromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
GFP-CYLD-C601A
Plasmid#60028PurposeN-terminal GFP tagged catalytic inactive (C601A) expressionDepositorInsertCylindromatosis (CYLD Human)
UseTagsGFPExpressionMammalianMutationCysteine 601 changed to AlaninePromoterCMVAvailable sinceNov. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-FOS
Plasmid#79511PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) Human FOS CDSDepositorInsertFOS (FOS Human)
UseMultisite gateway entry cloneTagsExpressionMutationPromoterno PromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
(p)odr-3::GFP::EGL-4
Plasmid#100897PurposeGFP and the EGL-4 cDNA driven by the odr-3 promoter, which expresses in the AWA, AWB, and AWC olfactory sensory neurons in C. elegans. unc-54 3'UTR in sequence as well.DepositorInsert56-2741: odr-3 promoter
UseTagsExpressionWormMutationPromoterodr-3Available sinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQ239
Plasmid#108859PurposeFnCpf1 Gateway entry plasmidDepositorInsertFnCpf1
UseCRISPR; Gateway compatible cpf1 entry cloneTagsNLSExpressionPlantMutationCpf1 is rice codon optimizedPromoterAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
KQ403: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-LSD1
Plasmid#121831PurposepMAGIC R4-R3 entry plasmid, contains 2x NLSx-dCas9(3.7) fused to the LSD1 H3K4me1/2 demethylase for 3 or 4-component MultiSite Gateway Pro assembly.DepositorInsertx-dCas9(3.7)/LSD1 (open)
UseSynthetic Biology; Pmagic gateway entry plasmidTagsNLSExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-CYFIP2.GFP
Plasmid#109140PurposeGFP-tagged CYFIP2 expression constructDepositorInsertCYFIP2 (CYFIP2 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-PrP (1-22)-BioID2-HA-13 x Linker- RBP(Z)-GPI-PSS
Plasmid#194755PurposeLentiviral expression of BioID2-HA-13 x Linker- RBP(Z) on the cell surface via a GPI anchorDepositorInsertPrP (1-22)-BioID2-HA-13 x Linker- RBP(Z)-GPI-PSS
UseLentiviralTagsHAExpressionMutationPromoterCMVAvailable sinceApril 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
p3E-DrCav1
Plasmid#194290PurposeMultisite gateway entry clone for expression of codon optimised zebrafish caveolin1 with fusion tag at the N-terminus. Parton lab clone KRTDepositorInsertcaveolin1 (cav1.S Frog)
UseMultisite gateway entry vectorTagsExpressionMutationPromoterAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKK-HA-TEV
Plasmid#105769PurposeExpression of your protein of interest in fusion with HA at the N-terminus. The tag is cleavable by TEV protease.DepositorTypeEmpty backboneUseFlp-in competentTagsHA-TEVExpressionMammalianMutationPromoterAvailable sinceFeb. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-CCL5.Pro
Plasmid#79492PurposeMultiSite Gateway entry clones, first fragment, (attL1, attR5) CCL5/RANTES promoterDepositorInsertCCL5 Promoter (CCL5 Synthetic, Human)
UseMultisite gateway entry cloneTagsExpressionMutationPromoterCCL5 PromoterAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
SPRR2A-pFastBac I
Plasmid#182460PurposeExpression His-tagged SPRR2A in insect cellsDepositorInsertsmall proline rich protein 2A (SPRR2A Human)
UseTags6xHisExpressionInsectMutationPromoterPolyhedrinAvailable sinceMarch 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-HA
Plasmid#105785PurposeExpression of your protein of interest in fusion with HA at the C-terminus. The tag is cleavable by TEV protease.DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-HAExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
KO101: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-Dnmt3a3L
Plasmid#121833PurposepMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) fused to the Dnmt3a-3L DNA methyltransferase for 3 or 4-component MultiSite Gateway Pro assembly.DepositorInsertx-dCas9(3.7)/Dnmt3a-3L (open)
UseSynthetic Biology; Pmagic gateway entry plasmidTagsNLSExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
MO91-ORAI1N223A-TEV-pHluorin (ORAI1-EC-pHluorin)
Plasmid#174336PurposeThe ORAI1N223A mutant containing-TEV-pHluorin-TEV sequence in the extracellular loop 2DepositorInserthuman Orai1 (ORAI1 Human)
UseRetroviralTagspHluorin inserted in the extracellular loop 2 of …ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT JunD-HA
Plasmid#58498PurposeGateway entry vector for an inducible HA-tagged mouse JunDDepositorInsertJunD (Jund Mouse)
UseEntry vectorTagsHAExpressionMutationPromoterAvailable sinceSept. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYPQ262m
Plasmid#161522PurposeGateway entry clone (attL1 & attR5) for CRISPR-zSpCas9 ABE7.10 mediated A-G base editingDepositorInsertTadA(wt)-TadA(7.10)-zSpCas9(D10A)
UseCRISPR; Gateway compatible tada(wt)-tada(7.10)-zs…TagsExpressionPlantMutationPromoterAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
STK16_HUMAN_D0
Plasmid#79701PurposeThis plasmid encodes the kinase domain of STK16. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertSTK16 (STK16 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pENTR3c GW CHD5
Plasmid#68869PurposeGW entry vector for CHD5DepositorInsertCHD5 (CHD5 Human)
UseEntry vectorTagsExpressionMutationPromoternoneAvailable sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pENTR-AtTAS1c-D2-B/c
Plasmid#137883PurposeGateway-compatible entry vector for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor at position downstream 3'D1[+]DepositorInsertAtTAS1c-D2-B/c
UseGateway-compatible entry cloneTagsExpressionMutationA. thaliana TAS1c precursor sequence including a …PromoterAvailable sinceMay 14, 2020AvailabilityAcademic Institutions and Nonprofits only