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PurposeEntry vector for human FGFR4
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 60531 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepENTR221
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 2546
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Vector typeEntry vector
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameFGFR4
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SpeciesH. sapiens (human)
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Insert Size (bp)2409
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Mutationc2134a P712T
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Entrez GeneFGFR4 (a.k.a. CD334, JTK2, TKF)
- Promoter NA
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pENTR221-FGFR4 was a gift from John Brognard (Addgene plasmid # 60531 ; http://n2t.net/addgene:60531 ; RRID:Addgene_60531) -
For your References section:
Targeted genetic dependency screen facilitates identification of actionable mutations in FGFR4, MAP3K9, and PAK5 in lung cancer. Fawdar S, Trotter EW, Li Y, Stephenson NL, Hanke F, Marusiak AA, Edwards ZC, Ientile S, Waszkowycz B, Miller CJ, Brognard J. Proc Natl Acad Sci U S A. 2013 Jul 23;110(30):12426-31. doi: 10.1073/pnas.1305207110. Epub 2013 Jul 8. 10.1073/pnas.1305207110 PubMed 23836671