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PM-FRB-mRFP-T2A-FKBP-5-ptase

Plasmid

#40896

Depositor

Peter Varnai

Article

Toth et al J Cell Sci. 2012 May 1;125(Pt 9):2185-97

Insert

PM-FRB-mRFP-T2A-FKBP-5-ptase

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 25, 2013

Availability

Academic Institutions and Nonprofits only

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ISceI-GR-RFP

Plasmid

#17654

Depositor

Tom Misteli

Article

Soutoglou et al Nat Cell Biol. 2007 Jun . 9(6):675-82.

Insert

I-SceI-GRLBD (SCEI, Nr3c1 Rat, Budding Yeast)

Use

Tags

mRFP

Expression

Mammalian

Mutation

C655G mutation in the glucocorticoid receptor (se…

Promoter

Available since

March 28, 2008

Availability

Academic Institutions and Nonprofits only

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pLV hUbC-dCas9-T2A-GFP

Plasmid

#53191

Purpose

Co-expresses human optimized S. pyogenes dCas9 and GFP

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Insert

humanized dead Cas9 T2A GFP

Use

CRISPR and Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

D10A and H840A

Promoter

hUbC

Available since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

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GFP- SEC61B

Plasmid

#121159

Purpose

Mammalian expression of GFP tagged SEC61B, used as a general ER marker

Depositor

Christine Mayr

Article

Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

Insert

SEC61B (SEC61B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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HA GSK3 beta K85A pcDNA3

Plasmid

#14755

Depositor

Jim Woodgett

Article

Hughes et al EMBO J. 1993 Feb . 12(2):803-8.

Insert

GSK3 beta K85A (GSK3B Human)

Use

Tags

HA

Expression

Mammalian

Mutation

K85A. Kinase dead mutant.

Promoter

Available since

April 12, 2007

Availability

Industry, Academic Institutions, and Nonprofits

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pFA6a-link-yomTagBFP2-Kan

Plasmid

#44899

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mTagBFP2 with Kan selection

Depositor

Kurt Thorn, Wendell Lim

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomTagBFP2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

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pEGFP-LC3 (human)

Plasmid

#24920

Purpose

Mammalian expression of LC3 fused to EGFP

Depositor

Toren Finkel

Article

Lee et al Proc Natl Acad Sci U S A. 2008 Mar 4. 10

Insert

LC3 (MAP1LC3B Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Tet2

Plasmid

#60939

Purpose

Expresses Tet2 in mammalian cells

Depositor

Yi Zhang

Article

Wang et al Cell Rep. 2014 Jan 30;6(2):278-84. doi:

Insert

Ten-Eleven Translocation 2 (Tet2 Mouse)

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pCE-GFP

Plasmid

#41858

Purpose

Non-integrating (episomal) expression of EGFP

Depositor

Shinya Yamanaka

Article

Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

Insert

eGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 24, 2013

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab7A T22N

Plasmid

#28048

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

Insert

Rab7A (RAB7A Human)

Use

Tags

2xHA and EGFP

Expression

Mammalian

Mutation

T22N

Promoter

Available since

May 27, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt3A

Plasmid

#35908

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt3A (WNT3A Human)

Use

Tags

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pQLinkH

Plasmid

#13667

Depositor

Konrad Buessow

Article

Scheich et al Nucleic Acids Res. 2007 Feb 20. ():.

Insert

multi cloning site

Use

Co-expression

Tags

His, TEV, and attB1

Expression

Bacterial

Mutation

Promoter

Available since

April 6, 2007

Availability

Academic Institutions and Nonprofits only

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pCS2-Gal4ff.zf1

Plasmid

#61392

Purpose

mRNA synthesis of Gal4ff codon modified for zebrafish

Depositor

Harold Burgess

Article

Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

Insert

Gal4ff.zf1

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

sp6

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

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TFEB promoter-luciferase reporter

Plasmid

#66801

Purpose

Reporter for activity of TFEB gene promoter

Depositor

Albert La Spada

Article

Tsunemi et al Sci Transl Med. 2012 Jul 11;4(142):142ra

Insert

TFEB promoter (Tfeb Mouse)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pOTTC407 - pAAV EF1a eGFP

Plasmid

#60058

Purpose

An AAV packaging vector that expresses enhanced green fluorescent protein under control of the EF1a promoter.

Depositor

Brandon Harvey

Article

Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

Insert

enhanced Green Fluorescent Protein

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Oct. 9, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-fNPY-GFP

Plasmid

#22912

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 . 3():19.

Insert

fugu neuropeptide Y promoter driving GFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 27, 2010

Availability

Academic Institutions and Nonprofits only

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PIK3CA - Exon20 H1047R

Plasmid

#16639

Depositor

Bert Vogelstein

Article

Samuels et al Science. 2004 Apr 23. 304(5670):554.

Insert

PIK3CA H1047R (PIK3CA Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

H1047R

Promoter

Available since

March 10, 2008

Availability

Academic Institutions and Nonprofits only

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5xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2

Plasmid

#158653

Purpose

making transgenics expressing a UAS driven mCherry and NTR 2.0

Depositor

Jeff Mumm

Article

Sharrock et al Nat Methods. 2022 Feb;19(2):205-215. doi

Insert

5xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y;he:BFP

Use

Tol2-based transgenic expression in zebrafish/oth…

Tags

Expression

Mutation

F70A/F108Y

Promoter

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFPN1-human Dynamin 1aa

Plasmid

#22163

Depositor

Pietro De Camilli

Article

Lee et al Proc Natl Acad Sci U S A. 2002 Jan 8. 99

Insert

human Dynamin 1aa (DNM1 Human)

Use

Tags

GFP

Expression

Bacterial and Mammalian

Mutation

D744N

Promoter

Available since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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pWPXL-Sox9

Plasmid

#36979

Depositor

Bob Weinberg

Article

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

Sox9 (Sox9 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

June 6, 2012

Availability

Academic Institutions and Nonprofits only

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