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pcDNA3-mCherry-Rheb

Plasmid

#192436

Purpose

Encodes mCherry-tagged wild-type Rheb.

Depositor

Jin Zhang

Article

Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

Insert

mCherry-Rheb (RHEB Human)

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pRPF185

Plasmid

#106367

Purpose

C. difficile inducible expression system. Shuttle plasmid containing a tetracycline-inducible gusA

Depositor

Robert Fagan, Neil Fairweather

Article

Fagan et al J Biol Chem. 2011 Aug 5;286(31):27483-93

Insert

Term(fdx)-Ptet-gusA-Term(slpA) (gusA )

Use

E. coli - c. difficile shuttle vector

Tags

Expression

Mutation

codon optimised for C. difficile

Promoter

Ptet

Available since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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mCitrine-N1

Plasmid

#54594

Purpose

Localization: N1 Cloning Vector, Excitation: 516, Emission: 529

Depositor

Robert Campbell, Michael Davidson, Oliver Griesbeck, Roger Tsien

Article

Griesbeck et al J Biol Chem. 2001 Aug 3;276(31):29188-94

Type

Empty backbone

Use

Tags

mCitrine

Expression

Mammalian

Mutation

Promoter

Available since

June 12, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pRGD-GmR

Plasmid

#74106

Purpose

Plasmid containing gentamicin resistance gene cassette flanked by multiple restriction sites

Depositor

Hans-Martin Fischer

Article

Ledermann et al Appl Environ Microbiol. 2016 Feb 26. pii

Insert

aacC1

Use

Bacterial vector based on pmb1 replicon

Tags

Expression

Mutation

Promoter

Available since

April 7, 2016

Availability

Academic Institutions and Nonprofits only

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pHTC-HaloTag_VPR:1-96

Plasmid

#211012

Purpose

Mammalian expression of full-length HV1N5 VPR. C-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

VPR:M1-S96

Use

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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Lenti-CMV-PEmax-IRES-ZeoR

Plasmid

#207353

Purpose

Lentiviral transfer plasmid containing CMV-driven expression cassette encoding PEmax enzyme for prime editing.

Depositor

Marianne Carlon

Article

Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

Insert

Prime editor max (PEmax) enzyme

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-FLEX-NEPLDCV-P2A-mRUBY3-WPRE

Plasmid

#207664

Purpose

Neuropeptide degradation

Depositor

Sung Han

Article

Kim et al bioRxiv 2023.01.19.524797

Insert

NEP (MME Human)

Use

AAV and Cre/Lox

Tags

mRUBY3

Expression

Mutation

Addition of signaling peptide (POMC) on the N-ter…

Promoter

hSynapsin

Available since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pX-RCC1-HA

Plasmid

#106938

Purpose

Transient expression of HA-tagged RCC1 in mammalian cells (CMV promoter)

Depositor

Patrizia Lavia

Article

DeLuca et al Cancer Res. 2003 Mar 15;63(6):1430-7.

Insert

human RCC1-HA (RCC1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

March 6, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV-IRES-Thy1.1 DEST

Plasmid

#17442

Purpose

Destination vector derived from MSCV-IRES-Thy1.1. Please note that this plasmid contains attB1 and attB2 sites for use in a BP reaction

Depositor

Anjana Rao

Article

Wu et al Cell. 2006 Jul 28. 126(2):375-87.

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only

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pET11phiC31poly(A)

Plasmid

#18942

Depositor

Michele Calos

Article

Groth et al Genetics. 2004 Apr . 166(4):1775-82.

Insert

phiC31 integrase (int Streptomyces phage phiC31)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 13, 2008

Availability

Academic Institutions and Nonprofits only

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MTK Lentivirus Multi Cassette Destination Vector (pAN2424)

Plasmid

#194221

Purpose

Backbone for constructing multi casette expression plasmids using the Mammalian Tookit

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv 2021.04.20.440678

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pKD3_mCherry

Plasmid

#187385

Purpose

To chromosomally tag genes of interest with mCherry. FRT-mCherry-cat-FRT

Depositor

Roi Avraham

Insert

mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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CoV2-N-R203M

Plasmid

#177952

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (R203M). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Nucleocapsid (R203M) (N )

Use

Tags

Expression

Mammalian

Mutation

R203M

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pLS-SceI

Plasmid

#137725

Purpose

Lentivirus reporter assay plasmid that contains a I-SceI site and a EGFP reporter gene.

Depositor

Nadav Ahituv

Article

Gordon et al Nat Protoc. 2020 Aug;15(8):2387-2412. do

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pKM200

Plasmid

#13079

Depositor

Kenan Murphy

Article

Murphy et al BMC Mol Biol. 2003 Dec 13. 4():11.

Insert

Ptac-gam-bet-exo operon

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 7, 2006

Availability

Academic Institutions and Nonprofits only

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mEGFP-Lifeact-7

Plasmid

#54610

Purpose

Localization: Actin, Excitation: 488, Emission: 507

Depositor

Michael Davidson

Type

Empty backbone

Use

Tags

mEGFP-Lifeact

Expression

Mammalian

Mutation

Promoter

Available since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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GFP-APEX2-NIK3x

Plasmid

#129274

Purpose

Expression of APEX2 in the nucleolus

Depositor

Alice Ting

Article

Fazal et al Cell. 2019 Jun 14. pii: S0092-8674(19)30

Insert

GFP-APEX2-NIK3x

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TRPM5

Plasmid

#85189

Purpose

Expresses full-length mouse TRPM5 in mammalian cells

Depositor

Craig Montell

Article

Hofmann et al Curr Biol. 2003 Jul 1;13(13):1153-8.

Insert

TRPM5 (Trpm5 )

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 22, 2017

Availability

Academic Institutions and Nonprofits only

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IMS-APEX2

Plasmid

#79058

Purpose

Expresses APEX in the mitochondrial intermembrane space.

Depositor

Alice Ting

Article

Hung et al Mol Cell. 2014 Jul 17;55(2):332-41. doi:

Insert

APEX

Use

Tags

V5

Expression

Bacterial and Mammalian

Mutation

Promoter

T7 promoter

Available since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pTfR-PAmKate

Plasmid

#32692

Depositor

Vladislav Verkhusha

Article

Gunewardene et al Biophys J. 2011 Sep 21;101(6):1522-8. Ep

Insert

PAmKate

Use

Tags

transferrin receptor (TfR)

Expression

Mammalian

Mutation

F84W/S148N/S165G compared to mKate (but numbering…

Promoter

CMV

Available since

Oct. 21, 2011

Availability

Academic Institutions and Nonprofits only

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