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Plasmid#18076DepositorInsertDystrophin (DMD Human)
UseTagsGSTExpressionBacterialMutationSecond conserved Tryptophan (W) of the WW domain …PromoterAvailable sinceMay 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2TK-WW-CR-W3083F/P3086A
Plasmid#18077DepositorInsertDystrophin (DMD Human)
UseTagsGSTExpressionBacterialMutationSecond conserved Tryptophan (W) of the WW domain …PromoterAvailable sinceMay 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2TK-WW-CR-d-ZZ-W3083F
Plasmid#18078DepositorInsertDystrophin (DMD Human)
UseTagsGSTExpressionBacterialMutationSecond conserved Tryptophan (W) of the WW domain …PromoterAvailable sinceMay 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2TK-WW-CR-d-ZZ-W3083F/P3086A
Plasmid#18079DepositorInsertDystrophin (DMD Human)
UseTagsGSTExpressionBacterialMutationSecond conserved Tryptophan (W) of the WW domain …PromoterAvailable sinceMay 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pHR-BTLA (Y226F, Y243F, Y257F, Y282F)-mGFP
Plasmid#180792PurposeExpression of human BTLA (Y226F, Y243F, Y257F, Y282F) fused to mGFPDepositorInsertBTLA (Y226F, Y243F, Y257F, Y282F) (BTLA Human)
UseLentiviralTagsExpressionMutationBTLA (Y226F, Y243F, Y257F, Y282F)PromoterSFFVAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-CyOFP1
Plasmid#105799PurposeExpression of your protein of interest in fusion with orange fluorescent protein at the C-terminus (cleavable by TEV) (PMID: 27240196). CyOFP1 is useful for multi-channel imaging.DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-CyOFP1ExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST_FLAG-HUWE1_UBA/UIM/ UBM-mut
Plasmid#187154Purposemammalian cell expression of FLAG-HUWE1 L2976A/P2977A/M1328A/F1330A/A1376G/S1382ADepositorInsertHUWE1 (HUWE1 Human)
UseTags1xFLAGExpressionMammalianMutationL2976A/P2977A/M1328A/F1330A/A1376G/S1382APromoterAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLv-HSA-uDys/eGFP
Plasmid#26810DepositorInsertdystrophin (DMD Human)
UseLentiviral; Lentiviral plasmid backbone from l. n…TagseGFPExpressionMutationdeleted R4-R23 deleted exons 71-78PromoterAvailable sinceSept. 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
pKK-CyOFP1-TEV
Plasmid#105782PurposeExpression of your protein of interest in fusion with orange fluorescent protein at the N-terminus (cleavable by TEV) (PMID: 27240196). CyOFP1 is useful for multi-channel imaging.DepositorTypeEmpty backboneUseFlp-in competentTagsCyOFP1-TEVExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
FHL-cpmTq2-Calcium-lifetime-sensor
Plasmid#129628PurposeGenetically encoded calcium sensor (GECI) that reports with lifetime and intensity changesDepositorInsertFHL-cpmTq2-Calcium-lifetime-sensor
UseTags6x His - Modified TorA MNNNDLFQASRRRFLAQLG[G to …ExpressionBacterial and MammalianMutationPromoterCMV,&RhamnoseAvailable sinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lck-cpmTq2-Calcium-lifetime-sensor
Plasmid#129627PurposeGenetically encoded calcium sensor (GECI) that reports with lifetime and intensity changesDepositorInsertLck-cpmTq2-Calcium-lifetime-sensor
UseTagsLckExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-DYRK1A-2A-GFP
Plasmid#118270PurposeExpresses mouse DYRK1A tagged with 2A peptide at it's C-terminus and GFP in mammalian cells.DepositorInsertdual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1a (Dyrk1a Mouse)
UseAAVTags2A peptide and GFPExpressionMammalianMutationPromoterCMV with beta global intronAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
3xnls-cpmTq2-Calcium-lifetime-sensor
Plasmid#129626PurposeGenetically encoded calcium sensor (GECI) that reports with lifetime and intensity changesDepositorInsert3xnls-cpmTq2-Calcium-lifetime-sensor
UseTags3xNLSExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
FSF-GFP
Plasmid#163658PurposeExpresses a TFR and ST6GAL1 swapped chimera with the cytosolic tail from TFR, TMD from ST6GAL1, and luminal domain from TFR in mammalian cellsDepositorInsertUseTagsGFPExpressionMammalianMutationThis chimera contains the cytosolic tail from TFR…PromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
SNS-GFP
Plasmid#163662PurposeExpresses a TNF and ST6GAL1 swapped chimera with the cytosolic tail from ST6GAL1, TMD from TNF and luminal domain from ST6GAL1 in mammalian cellsDepositorInsertUseTagsGFPExpressionMammalianMutationThis chimera contains the cytosolic tail from ST,…PromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
SFS-GFP
Plasmid#163655PurposeExpresses a TFR and ST6GAL1 swapped chimera with the cytosolic tail from ST6GAL1, TMD from TFR and luminal domain from ST6GAL1 in mammalian cellsDepositorInsertUseTagsGFPExpressionMammalianMutationThis chimera contains the cytosolic tail from ST,…PromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR-PD-1ecto&tmd-BTLAicd-mGFP
Plasmid#180794PurposeExpression of human PD-1 extracellular domain and transmembrane domain fused to BTLA intracellular domain followed by mGFPDepositorUseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
MLM3720
Plasmid#49944PurposeExpresses a mutant TALE-TET1CD (inactive catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene for use as a controlDepositorInsert3x FLAG Tet1CDmut RH-3 (RHOXF2 Human)
UseTALENTags3x FLAGExpressionMutationcatalytic domain of TET1 inactive (H1671Y and D16…PromoterEF1aAvailable sinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
MLM3709
Plasmid#49943PurposeExpresses a 3x Flag TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding siteDepositorInsert3x FLAG Tet1CD RH-3 (RHOXF2 Human)
UseTALENTags3x FLAGExpressionMutationPromoterEF1aAvailable sinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
MLM3713
Plasmid#49946PurposeExpresses a 3x Flag TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding siteDepositorInsert3x FLAG Tet1CD RH-4 (RHOXF2 Human)
UseTALENTags3x FLAGExpressionMutationPromoterEF1aAvailable sinceDec. 31, 2013AvailabilityAcademic Institutions and Nonprofits only