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Plasmid#109050PurposeCatalytically inactive dCasRx with 2A-EGFPDepositorInsertdCasRx
UseCRISPR and LentiviralTags2A-eGFP, HA, and NLSExpressionMammalianMutationR239A/H244A/R858A/H863APromoterAvailable sinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pACUH-GFP11x7-mCherry-α-tubulin
Plasmid#70218PurposeExpresses GFP11x7-mCherry-α-tubulin in insect cells. 5 a. a. linker lengths.DepositorInsertGFP11x7-mCherry-α-tubulin
UseTagsGFP11x7 and mCherryExpressionInsectMutationPromoterAvailable sinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDRM55 tet-AR(1-707)
Plasmid#183503PurposeLentiviral vector expressing a doxycycline-inducible constitutively active truncated androgen receptor (wild type AR)DepositorInsertAndrogen receptor (AR Human)
UseLentiviralTagsExpressionMammalianMutationConstitutively active truncated AR; spans AR 1-70…PromoterCMVAvailable sinceMay 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBABE GFP
Plasmid#10668PurposeEmpty backbone for retroviral gene expression. Select recipient cells using GFP.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2005AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2-let-7 8x
Plasmid#20931DepositorInsert8x let-7 target sites
UseLuciferaseTagsExpressionInsect and MammalianMutationPromoterAvailable sinceJune 19, 2009AvailabilityAcademic Institutions and Nonprofits only -
ACBE-Q
Plasmid#204608PurposeMammalian expression of high-accuracy adenine-to-cytosine base editor ACBE-QDepositorInsertbpNLS-N terminal of nSpCas9-linker-mAAG (R165E/Y179F)-TadA-8e (N108Q)-linker-C terminal of nSpCas9-bpNLS-P2A-GFP
UseCRISPRTags6*HisExpressionMammalianMutationTadA-8e (N108Q); mAAG (R165E/Y179F); S. pyogenes …PromoterAvailable sinceAug. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-FLEX-SaCas9-U6-sgOprk1
Plasmid#159903PurposeMutagenesis of Oprk1DepositorInsertOprk1 (Oprk1 Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CMV
Plasmid#72265PurposeExpress gene of interest under CMV promoterDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 25, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
mCherry-CD9-10
Plasmid#55013PurposeLocalization: Tetraspanin/Membrane, Excitation: 587, Emission: 610DepositorInsertCD9 (CD9 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EFS-PCP-GFPnls
Plasmid#121938PurposeCRISPR-Sirius plasmidDepositorInsertPCP-GFPnls
UseLentiviralTagssfGFPExpressionMammalianMutationPromoterEFSAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRDA_052
Plasmid#136474PurposeLentiviral expression of AsCas12a gRNADepositorInsertPuromycin resistance
UseLentiviral and Synthetic BiologyTagsExpressionMutationPromoterEF1aAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX330 Pten
Plasmid#59909PurposepX330 backbone expressing sgRNA targeting Pten to edit mouse Pten. Expresses Cas9 from CBh promoterDepositorInsertsgRNA targeting Pten (Pten Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMarch 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAM-kan-R4R3
Plasmid#71275PurposeBinary destination vector for plant transformation. Kanamycin resistance.DepositorTypeEmpty backboneUseMultisite gatewayTagsExpressionMutationPromoterAvailable sinceDec. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
multi-shRNA vector
Plasmid#12391DepositorInsertmSOD2 shRNA (Sod2 Mouse)
UseRNAiTagsExpressionMammalianMutationPromoterAvailable sinceSept. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLPC-NMYC TRF2
Plasmid#16066DepositorInserthTRF2 (TERF2 Human)
UseRetroviralTagsMycExpressionMutationaa2-500, inserted alanin at aa434PromoterAvailable sinceNov. 9, 2007AvailabilityAcademic Institutions and Nonprofits only -
TFORF2481
Plasmid#142948PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertMXD4 (MXD4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTRE-Tight-MitoTimer
Plasmid#50547PurposeExpresses the Timer protein targeted to the mitochondria under a Tet-inducible promoterDepositorInsertMitoTimer
UseTagsExpressionMammalianMutationF12S mutation in TimerPromoterAvailable sinceMarch 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
EGFP-E-Syt2
Plasmid#66831PurposeMammalian Expression, Bacterial ExpressionDepositorInsertE-Syt2 (ESYT2 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMma PylT_FLAG-AcKRS
Plasmid#140021PurposePlasmid with 4xMmaPylT cassette and MmaPylRS for amber suppression and incorporation of acetyl-lysine; for transient or stable piggyBac-mediated integrationDepositorInsertAcKRS-3 (pylS Methanosarcina mazei)
UseTagsFLAGExpressionMammalianMutationL301M, L305I, Y306F, L309A, C348FPromoterEF1Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBb(RSF1010)1k-GFPuv
Plasmid#106395PurposeIPTG-inducible trc promoter expressing gfpuv on RSF1010 originDepositorInsertGFPuv
UseTagsExpressionBacterialMutationPromotertrcAvailable sinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits only