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pAAV-Ef1a-DIO-ChETA-TdTomato-WPRE-pA

Plasmid

#37755

Purpose

Cre-dependent expression of ChETA-tdTomato, for optogenetic excitation

Depositor

Bernardo Sabatini

Article

Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

Insert

ChETA-TdTomato

Use

AAV and Cre/Lox; Cre-on

Tags

TdTomato

Expression

Mutation

Promoter

EF1a

Available since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

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AAV-CAG::FLEX-rev:: ChR2HA-2a-hM4D

Plasmid

#52521

Purpose

Co-expresses HA tagged channelrhodopsin and hM4D from a Cre-dependent virus

Depositor

Scott Sternson

Article

Stachniak et al Neuron. 2014 May 21;82(4):797-808. doi:

Insert

ChR2-2a-hM4D

Use

AAV and Cre/Lox

Tags

2xHA

Expression

Mutation

Promoter

Available since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-CamKII-GFP-P2A-bReaches-minWPRE

Plasmid

#118276

Purpose

Expresses EGFP and bReaChES under a minimal CamKII Promoter

Depositor

Dietmar Schmitz

Article

Bernal Sierra et al Nat Commun. 2018 Nov 5;9(1):4611. doi: 1

Inserts

EGFP
bReaCHES

Use

AAV

Tags

P2A

Expression

Mutation

Promoter

CamK II

Available since

Dec. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-EnhancedSynapsin-DIO-TVA950-EYFP-WPRE

Plasmid

#120269

Purpose

Allows for initial cellular transduction of EnvA-pseudotyped Rb via Cre-dependent expression of an avian TVA receptor fused with yellow fluorescent protein

Depositor

Edward Callaway

Article

Faget et al Cell Rep. 2016 Jun 21;15(12):2796-808. d

Insert

eSyn-DIO-TVA950:YFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-Scrambled-hSyn::mCherry.3xFLAG-WPRE

Plasmid

#120395

Purpose

pAAV plasmid expressing scrambled shRNA under the U6 promoter and mCherry.3xFLAG under the hSyn promoter

Depositor

Florian Freudenberg

Article

Grunewald et al J Atten Disord. 2019 Jan 9:1087054718822

Insert

Scrambled shRNA

Use

AAV and RNAi

Tags

mCherry.3XFLAG

Expression

Mutation

Promoter

U6

Available since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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AAV8-hSyn-flex-miR30-eGFP-shCrh

Plasmid

#132715

Purpose

Encodes Cre-dependent short hairpin RNA (shRNA) that targets the 3’-untranslated region of the rat Crh gene which encodes corticotropin releasing factor

Depositor

Robert Messing

Article

Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

Insert

GFP-shCrh (Crh Rat)

Use

AAV

Tags

Expression

Mutation

Promoter

Synapsin 1

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-FLEX-NES-jGCaMP8f-WPRE

Plasmid

#186044

Purpose

AAV-mediated cytosolic expression of jGCaMP8f under the CAG promoter, Cre-dependent expression

Depositor

Loren Looger

Insert

NES-jGCaMP8f

Use

AAV

Tags

NES-6xHis

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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1782_pAAV-U6-Ai9-Sa-gRNA2-CB-SACas9-HA-OLLAS-spA_C

Plasmid

#135978

Purpose

gRNA against the right loxP site of the Ai9 allele

Depositor

William Lagor

Insert

Ai9 gRNA2

Use

AAV, CRISPR, and Mouse Targeting

Tags

HA

Expression

Mutation

Promoter

CB

Available since

Jan. 23, 2020

Availability

Academic Institutions and Nonprofits only

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1781_pAAV-U6-Ai9-Sa-gRNA1-CB-SACas9-HA-OLLAS-spA_C

Plasmid

#135979

Purpose

gRNA against the left loxP site of the Ai9 allele

Depositor

William Lagor

Insert

Ai9 gRNA1

Use

AAV, CRISPR, and Mouse Targeting

Tags

HA

Expression

Mutation

Promoter

U6

Available since

Jan. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV syn CCR6-eLOV-TEVcs-tTA

Plasmid

#173116

Purpose

Expresses CCR6 receiver construct in neurons

Depositor

Alice Ting

Article

Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

Insert

CCR6-eLOV-TEVcs-tTA

Use

AAV

Tags

Expression

Mutation

Promoter

Synapsin

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Camk2a(0.4)-Lyn-PHR(E281A)-HA

Plasmid

#180591

Purpose

Control for Opto-cytTrkB

Depositor

Won Do Heo

Article

Hong J et al J Mol Biol. 2020 Feb 14;432(4):815-827.

Insert

PHR

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α1.1-Jaws-KGC-tdTomato-ER2

Plasmid

#153538

Purpose

AAV-mediated expression of Jaws-tdTomato-ER2 under the EF1α promoter (1.1kb short version).

Depositor

Edward Boyden

Article

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Insert

Jaws-KGC-tdTomato-ER2

Use

AAV

Tags

ER2, KGC, and tdTomato

Expression

Mammalian

Mutation

K200R W214F

Promoter

EF1α1.1

Available since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-FRT-FLEX-GtACR2-EYFP

Plasmid

#114369

Purpose

expresses Flpo recombinase-dependent GtACR2

Depositor

Mingshan Xue

Article

Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

Insert

GtACR2-EYFP (NEWENTRY )

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-FLEX-NES-jGCaMP8m-WPRE

Plasmid

#186045

Purpose

AAV-mediated cytosolic expression of jGCaMP8m under the CAG promoter, Cre-dependent expression

Depositor

Loren Looger

Insert

NES-jGCaMP8m

Use

AAV

Tags

NES-6xHis

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-dSa-VPR mini.-syn pA

Plasmid

#99689

Purpose

Expresses dSa Cas9 fused to optimized combination of truncated activation domains with syn pA (poly adenylation) signal

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

AAV

Tags

VPR mini

Expression

Mutation

dead Cas9

Promoter

Available since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-Archon1-KGC-EGFP-ER2

Plasmid

#108419

Purpose

AAV production plasmid encoding for Archon1 fluorescent voltage reporter

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

Archon1-KGC-EGFP-ER2

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CaMKII

Available since

April 11, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV_hsyn_NES-his-CaMPARI2-H396K-WPRE-SV40

Plasmid

#101062

Purpose

AAV vector expressing CaMPARI2_H396K (Kd = 360nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

Depositor

Eric Schreiter

Article

Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

Insert

CaMPARI2-H396K

Use

AAV

Tags

FLAG-HA-myc and NES_his

Expression

Mutation

Promoter

hsyn (synapsin-1)

Available since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-Hb9-LMO3 (sbGLuc-VChR1-EYFP)

Plasmid

#114103

Purpose

fusion protein of Gaussia luciferase variant slow burn, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogenetics

Depositor

Ute Hochgeschwender

Article

Petersen et al Front Neurol. 2022 Jan 20;12:792643. doi

Inserts

Gaussia luciferase, slow burn
Volvox Channelrhodopsin 1
Enhanced Yellow Fluorescent Protein

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-DIO-CaMKII-TVA-P2A-N2cG

Plasmid

#172362

Purpose

Targeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling from cre-expressing excitatory cortical neurons

Depositor

Peter Jonas, Yoav Ben-Simon

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

TVA + B19-N2cG

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

CaMKIIa

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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SL016: AAV-CAG-FLEX-CheRiff-GFP

Plasmid

#124776

Purpose

For AAV based expression of CheRiff in Cre-Positive cells

Depositor

Adam Cohen

Article

Farhi et al J Neurosci. 2019 Apr 5. pii: JNEUROSCI.0

Insert

CheRiff-GFP

Use

AAV and Cre/Lox

Tags

GFP

Expression

Mutation

Promoter

CAG

Available since

June 7, 2019

Availability

Academic Institutions and Nonprofits only