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Addgene
Showing: 3281 - 3300 of 4263 results
  1. pAAV-hSyn-sbGLuc-ChR2(CS/DA)-EYFP

    Plasmid
    #114106
    Purpose
    fusion protein of Gaussia luciferase variant slow burn, Channelrhodopsin-2 variant C128S/D156A (stabilized step function opsin), and enhanced yellow fluorescent protein for bioluminescent optogenetics
    Inserts
    Gaussia luciferase, slow burn
    Channelrhodopsin 2(CS/DA), stable step function opsin
    Enhanced Yellow Fluorescent Protein
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  2. pAAV-hSyn-sbGLuc-B7-EYFP

    Plasmid
    #114110
    Purpose
    fusion protein of Gaussia luciferase variant slow burn, B7 transmembrane region, and enhanced yellow fluorescent protein; control for bioluminescent optogenetics
    Inserts
    Gaussia luciferase, slow burn
    B7 hinge region
    Enhanced Yellow Fluorescent Protein
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  3. pcDNA3.1-CAG-sbGLuc-hGtARC2-EYFP

    Plasmid
    #114111
    Purpose
    fusion protein of Gaussia luciferase variant slow burn, Guillardia theta anion channelrhodopsin-2, and enhanced yellow fluorescent protein for bioluminescent optogenetics
    Inserts
    Gaussia luciferase, slow burn
    Guillardia theta anion channelrhodopsin-2, human codon optimized
    Enhanced Yellow Fluorescent Protein
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  4. pLV-pGK-Nluc-Fluc-NLS-P2A-H2B-mCherry-IRES-Hygro

    Plasmid
    #115684
    Purpose
    NanoLuc-Firefly Luciferase fusion with constitutive nuclear expression for calibration of Nanoluc signal to Firefly Luciferase signal. H2B-mCherry marks chromatin with red fluorescence.
    Depositor
    Insert
    Nluc-Fluc-NLS-P2A-H2B-mCherry-IRES-Hygro
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  5. p(A)n-PAUSE

    Plasmid
    #118068
    Purpose
    Transcription Blocker - Synthetic polyadenylation signal & RNA polymerase II transcriptional pause signal from the human α2 globin gene
    Depositor
    Insert
    Synthetic polyadenylation signal & RNA polymerase II transcriptional pause signal from the human α2 globin gene
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  6. pAF112

    Plasmid
    #135920
    Purpose
    All-in-one Sleeping Beauty vector for stable transfection of eukaryotic cells with luciferse and hygromycin restistance. CMV protomor drives SB100X transposase for transposon integration.
    Depositor
    Type
    Empty backbone
    Use
    Luciferase; Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
Showing: 3281 - 3300 of 4263 results