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Plasmid#84484PurposeAAV-mediated expression of Chronos-tdTomato under the CAG promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. tdTomato is a codon diversified version.DepositorInsertChronos-tdTomato
UseAAVTagstdTomatoExpressionMammalianMutationPromoterCAGAvailable sinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
TFORF2545
Plasmid#144038PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertCEBPB (CEBPB Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
NbV5-mCherry
Plasmid#201474PurposeIndirect microscopy/imaging of V5-tagged proteinDepositorInsertNbV5-mCherry
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-XL4 FLAG-NGRN-Fc
Plasmid#115773PurposeExpression of the extracellular domain of NGRN with N-terminal FLAG tag and C-terminal hIgG tag.DepositorInsertNGRN (NGRN Human)
UseTagsFLAG and hIgG/FcExpressionMammalianMutationnonePromoterCMVAvailable sinceOct. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pPUR-mU6-sgRNA-2XPP7
Plasmid#194504PurposeEmpty backbone for sgRNA-2XPP7 in CRIPSR FISHer systemDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/Luc2=tdT
Plasmid#32904PurposeExpresses a fusion of firefly luciferase and tandem Tomato red fluorescent protein in mammalian cells.DepositorInsertsFirefly Luciferase
tandem Tomato Red Fluorescent Protein
UseTagstdTomato red fluorescent proteinExpressionMammalianMutationPromoterCMV-IE and n/aAvailable sinceFeb. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FKBP-MAVS(pLxIS)
Plasmid#221256PurposeExpression of MAVS pLxIS (393-441) in mammalian cells by retroviral transductionDepositorInsertMAVS pLxIS (393-441) (Mavs Mouse)
UseRetroviralTagsFLAG-4xFKBPExpressionMutationPromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
Flag-GFP11-pDEST-N
Plasmid#118366PurposeBimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible N-terminal vector with BiFC-tag (GFP11-Flag) to screen interaction partners.DepositorTypeEmpty backboneUseDestinationTagsGFP11-FlagExpressionMammalianMutationPromoterAvailable sinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pλPR cI-Elysis
Plasmid#176891PurposeBacterial expression plasmids for production of E. coli bacterial ghost (BG) capsulesDepositorInsertLysis E gene from PhiX174 and lambda PR-cI857 regulatory system
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-Myoferlin HA
Plasmid#22443DepositorInsertMyoferlin
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceDec. 8, 2009AvailabilityAcademic Institutions and Nonprofits only -
pRRLSIN.cPPTLuciferase.WPRE
Plasmid#69251Purposeluciferase reporter in lentivirus backboneDepositorInsertLuciferase
UseLentiviralTagsExpressionMutationPromoternoneAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTD103luxI_sfGFP
Plasmid#48885PurposePositive feedback plasmid from oscillatorDepositorInsertLuxI, sfGFP, and LuxR
UseSynthetic BiologyTagsssrA tag (AANDENYALAA)ExpressionBacterialMutationPromoterpLuxAvailable sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAG_PSD95.FingR-eGFP-CCR5TC
Plasmid#46295DepositorInsertPSD95.FingR-eGFP-CCR5TC (DLG4 Human, Synthetic)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCE-KdGl
Plasmid#154880PurposeEpisomally expresses KDM4D and GLIS1 in mammalian cellsDepositorUseTagsExpressionMammalianMutationPromoterAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHrD-IRES-Fluc
Plasmid#194250PurposeHuman RNAP1 promoter region driving firefly luciferase expression from IRES. Constructed by Ghoshal, et al. J Biol Chem (2004). Please cite this original manuscript!DepositorInsertFirefly luciferase
UseLuciferaseTagsExpressionMutationPromoterhuman rDNA promoter and beginning of 5' ETS …Available sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBJB20 - eft-3p::mitofilin::Mac::GFP::unc-54 3'UTR
Plasmid#158967PurposeUbiquitous expression of mtON in C. elegansDepositorInserteft-3p::mitofilin::Mac::GFP::unc-54 3'UTR
UseTagsGFPExpressionWormMutationPromotereft-3Available sinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
Flag-HA-UCHL3
Plasmid#22564DepositorInsertUCHL3 (UCHL3 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationNonePromoterAvailable sinceNov. 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-K29R
Plasmid#17602PurposeMammalian expression of HA tagged ubiquitin K29RDepositorInsertUbiquitin C (UBC Human)
UseTagsHAExpressionMammalianMutationK29R; V5 deletedPromoterAvailable sinceMarch 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLV-mScarlet-I
Plasmid#159172PurposeTo express a bright monomeric red FP for labelling and detection of mammalian cellsDepositorInsertmScarlet-I
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationCodons were optimized to Human codon usagePromoterCMVAvailable sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEMS-II-PEST
Plasmid#204766PurposeGEMS 2.0 sensor system with PEST destabilization domain (pCMV-EGFP-PEST-m6Areporter-DHFR, Ef1a-APO1-YTH, hPGK-DsRed) (pFM577+PEST)DepositorInsertEGFP-PEST-m6Alinker-DHFR
UseTagsExpressionMutationmutated all RACs in EGFP to RGC/RCCPromoterCMVAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF0983
Plasmid#143739PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertKLF5 (KLF5 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFR_CrPV_xb
Plasmid#11509DepositorInsertFirefly luciferase, Cricket Paralysis Virus IRES sequence, Renilla, and 3'UTR short RNA binding sites
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceMarch 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_PIK3CA_WT
Plasmid#81736PurposeGateway Donor vector containing PIK3CA , part of the Target Accelerator Plasmid Collection.DepositorInsertPIK3CA (PIK3CA Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCE-K2N
Plasmid#154879PurposeEpisomally expresses KLF2 and NANOG in mammalian cellsDepositorUseTagsExpressionMammalianMutationPromoterAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTol1-UAS:CoChR-tdTomato
Plasmid#124233PurposeZebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin CoChR fused to the red fluorescent protein tdTomatoDepositorInsertCoChR-tdTomato
UseZebrafish expressionTagstdTomatoExpressionMutationPromoter14xUAS-E1bAvailable sinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-2xPH-TAPP1 WT
Plasmid#161990PurposeExpresses two tandem repeats of Tapp1 PH domain that binds to PI(3,4)P2. Fused to eGFPDepositorInsertPH-TAPP1 (PLEKHA1 Human)
UseTagseGFPExpressionMammalianMutationPromoterAvailable sinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV.GfaABC1D.PI.Cre.SV40
Plasmid#105603PurposeAAV expression of Cre from GfaABC1D promoterDepositorInsertCre
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterGfaABC1DAvailable sinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-Trastuzumab-IgG2/κ
Plasmid#61884PurposeExpresses HER2/neu receptor specific humanized IgG2/κ antibody isotype (Trastuzumab-IgG2/κ)DepositorInsertsHER2/neu receptor specific humanized Gamma 2 heavy chain expression cassette
HER2/neu receptor specific humanized kappa light chain expression cassette
UseTagsExpressionMammalianMutationPromotermEF1 Prom and rEF1 PromAvailable sinceMarch 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/Flag-METTL3
Plasmid#53739PurposeFor expression of METTL3 (methyltransferase-like 3) in human cellsDepositorInsertMethyltransferase-like 3 (METTL3 Human)
UseTagsFLAG tagExpressionMammalianMutationPromoterT7Available sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-DNAseI
Plasmid#182409PurposeBacterial expression of Vesicle Nucleating peptide-DNaseI fusionDepositorInsertVNp-DNase I (DNASE1 Bovine)
UseTagsVesicle Nucleating peptide (VNp)ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLminP_Luc2P_RE60
Plasmid#90405PurposeSTAT5 - Stat5 gene reporterDepositorInsertFirefly luciferase
UseLentiviralTagsExpressionMutationPromoterSIEAvailable sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pRL528
Plasmid#58495Purposehelper plasmid for bacterial conjugal DNA transferDepositorInsertencodes methylases AvaiM and Eco47iiM that target sites subject to restriction in Anabaena sp. strain PCC 7120
UseTagsExpressionMutationPromoterAvailable sinceOct. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV.GFAP.Cre.WPRE.hGH
Plasmid#105550PurposeAAV expression of Cre from GFAP promoterDepositorHas ServiceAAV PHP.eB and AAV5InsertCre
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterGFAPAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
V5-BS2-NES (Cytosol)
Plasmid#220341PurposeMammalian expression vector BS2 in cytoplasmDepositorInsertBS2
UseTagsNES (Nuclear exclusion sequence) and V5 tagExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJSK663
Plasmid#205129PurposeExpression of GFP-1xPX (from SnxA) in mammalian cellsDepositorInsert1xPX domain of SnxA
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceSept. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-Cre
Plasmid#196140PurposeAAV-mediated expression of Cre under the CAG promoter .DepositorInsertCre
UseAAVTagsExpressionMammalianMutationcodon diversifiedPromoterCAGAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pF150 pSOD1G93AAcGFP1
Plasmid#26411Purposecontains G94A mutation in pSOD1DepositorInsertSOD1 (SOD1 Human)
UseTagsGFPExpressionMammalianMutationG94APromoterAvailable sinceNov. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
pEGFP PICH (Nigg CB62)
Plasmid#41163DepositorInsertPICH (ERCC6L Human)
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-Guide-EFS-SpCas9-P2A-EGFP
Plasmid#99248Purposesingle-vector system with EGFP reporter for use in CROPseq knockout screensDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsEGFPExpressionMammalianMutationPromoterEFSAvailable sinceNov. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAC-ZEAXipi
Plasmid#53287PurposeContains crtE, idi, crtI, crtY, crtB, and crtZ genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces zeaxanthin in E. coliDepositorInsertcrtE, idi, crtY, crtI, crtB, crtZ
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterendogenous promotersAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDRM210 LGP (Luciferase GFP Puro)
Plasmid#174722PurposeExpresses the firefly luciferase gene, E2A, eGFP, F2A, and the puromycin resistance geneDepositorInsertsFirefly Luciferase
eGFP
pac
UseLentiviral and LuciferaseTagsE2A linker and F2A linkerExpressionMammalianMutationPromoterMND, MND (off Luc-E2A), and MND (off Luc-E2A-eGFP…Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAW12.lentiguide.GFP
Plasmid#104374PurposeGFP vector for cloning in guidesDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-Uch37
Plasmid#19415DepositorInsertUch37 (UCHL5 Human)
UseTags2XHAExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
T4 td upstream intron-PABP
Plasmid#189972PurposePart 1 plasmid to introduce the T4 td upstream intron and PABP spacer into circRNAsDepositorInsertT4 td upstream intron-PABP
UseCloningTagsExpressionMutationPromoterN/AAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL4.20-HIF1A prom
Plasmid#40173DepositorInsertHIF1A promoter fragment (HIF1A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SLCO1B1_STOP
Plasmid#161354PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLCO1B1 (SLCO1B1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEE515
Plasmid#149288PurposeT-DNA encoding TRV2 complete genome with MCSDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1_hTRIM32
Plasmid#69541PurposeExpresses human TRIM32 fused to c-terminal EGFPDepositorInserttripartite motif containing 32 (TRIM32 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only