Showing: 3281 - 3300 of 18067 results
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Plasmid#74466Purposeexpression of hVEGF165 in mammalian cellsDepositorInsertvascular endothelial growth factor 165 (VEGFA Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCR3.1-mIL2
Plasmid#74467Purposeexpression of mouse IL2 in mammalian cellsDepositorInsertIL2 (Il2 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-Cortactin
Plasmid#74476PurposeThis plasmid was constructed to study cortactin interactionsDepositorInsertCortactin (Cttn Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pSG4K5
Plasmid#74492PurposeFor cloning and constitutive expression of sgRNAs in E.coli. New sgRNAs are cloned into SapI sites and multiplex sgRNAs are cloned into BsaI sites. Kanamycin resistant with pSC101 origin of rep.DepositorInsertsgRNA
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23119AvailabilityAcademic Institutions and Nonprofits only -
pCas9(D10A)
Plasmid#74495PurposeNicking Cas9 derived from pWT Cas9 (Addgene #44250). Ampicillin resistance marker, the tetracycline repressor (TetR), a ColE1 origin of replication and Streptococcus pyogenes Cas9 D10ADepositorInsertCas9 D10A
UseSynthetic BiologyTagsExpressionBacterialMutationD10APromoterpTetoAvailabilityAcademic Institutions and Nonprofits only -
pMSCV-Flt3-ITD-Y591F/Y919F
Plasmid#74499PurposeRetroviral expression of FLT3 ITD-Y591F/Y919FDepositorInsertFLT3 (FLT3 Human)
UseRetroviralTagsExpressionMammalianMutationY591F/Y919F, T227MPromoterunknownAvailabilityAcademic Institutions and Nonprofits only -
pcFLAG-CSK-WT
Plasmid#74502PurposeOverexpression of CSK in mammalian cellsDepositorInsertCSK (CSK Human)
UseTagsFLAGExpressionMammalianMutationK222RPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcFLAG-FYN-WT
Plasmid#74509PurposeOverexpression of FYN in mammalian cellsDepositorInsertFYN (FYN Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn1-EGFP-P2A-EGFPf-WPRE-HGHpA
Plasmid#74513PurposeAAV vector that use human synapsin-1 promoter to drive the expression of EGFP and membrane-targeted EGFPf linked by self-cleaving P2A peptide.DepositorInsertEGFP-p2A-EGFP-f
UseAAVTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pKHR4
Plasmid#74592Purposean ampR plasmid built from pBluescript: the multiple cloning site (mcs) flanked by inverted head-to-head oriented I-SceI sites. Genbank accession number is KU144822.DepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pKHR5
Plasmid#74593Purposean alpha-crystallin::Venus (green lens) reporter cassette flanked by FRT sites and with a single loxP site at the 3’ end was inserted in the middle of pKHR4 mcs. Genbank accession number is KU144823.DepositorInsertFRT-alpha-crystallin::Venus-FRT-loxP reporter cassette
UseCre/Lox; Zebrafish expressionTagsExpressionMutationPromoteralpha-crystallinAvailabilityAcademic Institutions and Nonprofits only -
pET28a-RILP
Plasmid#74600Purposeused for expression in E. coli and purificationDepositorInsertRab-interacting lysosomal protein (RILP Human)
UseTagsFLAG, StrepII, and superfolder GFPExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pHR-mCherry-p2A-full length LIC1
Plasmid#74601Purposeexpresses LIC1 in mammalian cells; untagged but cells indicate expression with diffuse mCherryDepositorInsertfull length human dynein light intermediate chain 1 (DYNC1LI1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailabilityAcademic Institutions and Nonprofits only -
pKHR7
Plasmid#74624Purposea cmlc2::mCherry (red heart) reporter gene was inserted at the 3’end of pKHR4 mcs, that resides within donor fragments produced by I-SceI digestion. Genbank accession number is KU144824.DepositorInsertcmlc2::mCherry
UseZebrafish expressionTagsExpressionMutationPromotercmlc2AvailabilityAcademic Institutions and Nonprofits only -
pKHR8
Plasmid#74625Purposea plasmid derived from pKHR5: in addition to Venus reporter gene, it also contains cmlc2::mCherry (red heart) reporter gene at 3’ end of the mcs as pKHR7. Genbank accession number is KU144825.DepositorInsertsFRT-alpha-crystallin::Venus-FRT-loxP reporter cassette
cmlc2::mCherry
UseCre/Lox; Zebrafish expressionTagsExpressionMutationPromoteralpha-crystallin and cmlc2AvailabilityAcademic Institutions and Nonprofits only -
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AIO-Puro
Plasmid#74630PurposeAll-in-One plasmid encoding dual U6 promoter-driven sgRNAs and Cas9-D10A nickase linked via 2A peptide with puromycin resistant marker to enhance efficient and accurate genome editingDepositorTypeEmpty backboneUseCRISPRTagsPuromycin resistant markerExpressionMammalianMutationPromoterCbhAvailabilityAcademic Institutions and Nonprofits only -
p5E--3mnx1
Plasmid#74632PurposeCan be used to drive expression specifically in zebrafish motor neuronsDepositorInsertmotor neuron and pancreas homeobox 1 promoter (mnx1 Zebrafish)
Use5' entry vector for multisite gateway three …TagsExpressionMutationPromoter-3mnx1AvailabilityAcademic Institutions and Nonprofits only -
pCX-DI-2A
Plasmid#74671PurposeMammalian expression of DI-2A (hCD73/F2A/hCD39)DepositorUseTagsF2AExpressionMammalianMutationPromoterpCAGGSAvailabilityAcademic Institutions and Nonprofits only -
pCX-HO1-2A-EGFP
Plasmid#74672PurposeMammalian expression of HO1 and EGFPDepositorInsertHO1 (HMOX1 Human)
UseTags2A-EGFPExpressionMammalianMutationPromoterpCAGGSAvailabilityAcademic Institutions and Nonprofits only
Showing: 3281 - 3300 of 18067 results