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Plasmid#144071PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertUNCX (UNCX Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
MSCV-dGFP-JARID1C
Plasmid#74776Purposeretroviral expression of JARID1CDepositorInsertJARID1C (KDM5C Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-MICU1-mEF
Plasmid#31765DepositorInsertMICU1 (MICU1 Human)
UsePdonr gatewayTagsNoneExpressionMutationMutated in both EF-hand domains: D231A, E242K, D4…PromoterAvailable sinceMay 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-55:DMD-mEGFP
Plasmid#159744PurposeHomology arms and linker-mEGFP sequence for C-terminus tagging of human DMD via Cas9-excisable CAGGS-mCherry selection cassetteDepositorInsertDMD Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (DMD Human)
UseCRISPR; Donor templateTagslinker-mEGFPExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJFA344C7
Plasmid#49236PurposeTET1-catalytic domain (CD) expression vector for TALE-TET1 fusion protein to induce targeted demethylation in mammalian cellsDepositorInsertTALE-TET1CD Backbone (TET1 Human, Synthetic)
UseTALEN; Tale-tet1 expression vectorTagsExpressionMutationPromoterEF1aAvailable sinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SynI-FlpOn-CreOff-MCS-P2A-mCherry
Plasmid#176277PurposeViral vector for co-expression of a CDS of interest and mCherry in cells expressing Flp AND NOT Cre driven by a Synapsin promoter. Contains an in-frame P2A sequence and an MCS for cloning of the CDS.DepositorInsertMCS-P2A-mCherry
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterhuman Synapsin IAvailable sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJFA334E9
Plasmid#49237PurposeTET1-catalytic domain (CD) expression vector for TALE-TET1 fusion protein to induce targeted demethylation in mammalian cellsDepositorInsertTET1 tet methylcytosine dioxygenase 1 (TET1 Human, Synthetic)
UseTALEN; Tale-tet1 expression vectorTagsExpressionMutationPromoterEF1aAvailable sinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDEST_FLAG-HUWE1_BH3-4E
Plasmid#187144Purposemammalian cell expression of FLAG-HUWE1 V1976E/V1780E/L1983E/M1987EDepositorInsertHUWE1 (HUWE1 Human)
UseTags1xFLAGExpressionMammalianMutationV1976E/V1780E/L1983E/M1987EPromoterAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST_FLAG-HUWE1_delta3291-3298
Plasmid#187125Purposemammalian cell expression of FLAG-HUWE1 delta3291-3298DepositorInsertHUWE1 (HUWE1 Human)
UseTags1xFLAGExpressionMammalianMutationdelta3291-3298PromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
TFORF3452
Plasmid#144928PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZNF460 (ZNF460 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRC/CMV-TYK2V678F-VSV
Plasmid#139349PurposeExpression of a gain of function TYK2-V678F protein (correspoding to JAK2-V617F)DepositorInsertTYK2 cDNA with 267nt of 5' UTR (TYK2 Human)
UseTagsVSVExpressionMammalianMutationV362F-V678FPromoterCMVAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX330A-Yap1-E1-#1
Plasmid#171517Purposedeletion of a genomic locus in Yap1 geneDepositorInsertYap1 (Yap1 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX330A-Yap1-E2-#1
Plasmid#171518Purposedeletion of a genomic locus in Yap1 geneDepositorInsertYap1 (Yap1 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJFA274
Plasmid#49239PurposeTET1-catalytic domain (CD) expression vector for TALE-TET1 fusion protein to induce targeted demethylation in mammalian cellsDepositorInsertTET1 tet methylcytosine dioxygenase 1 (TET1 Human, Synthetic)
UseTALEN; Tale-tet1 expression vectorTagsExpressionMutationPromoterEF1aAvailable sinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SynI-CreOn-FlpOff-MCS-P2A-EGFP
Plasmid#176276PurposeViral vector for co-expression of a CDS of interest and EGFP in cells expressing Cre AND NOT Flp driven by a Synapsin promoter. Contains an in-frame P2A sequence and an MCS for cloning of the CDS.DepositorInsertMCS-P2A-EGFP
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterhuman Synapsin IAvailable sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
FSS-GFP
Plasmid#163654PurposeExpresses a TFR and ST6GAL1 swapped chimera with the cytosolic tail from TFR and TMD and luminal domain from ST6GAL1 in mammalian cellsDepositorInsertUseTagsGFPExpressionMammalianMutationThis chimera contains the cytosolic tail from TFR…PromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
SSF-GFP
Plasmid#163656PurposeExpresses a TFR and ST6GAL1 swapped chimera with the cytosolic tail and TMD from ST6GAL1, and luminal domain from TFR in mammalian cellsDepositorInsertUseTagsGFPExpressionMammalianMutationThis chimera contains the cytosolic tail from ST,…PromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
FFS-GFP
Plasmid#163659PurposeExpresses a TFR and ST6GAL1 swapped chimera with the cytosolic tail and TMD from TFR, and luminal domain from ST6GAL1 in mammalian cellsDepositorInsertUseTagsGFPExpressionMammalianMutationThis chimera contains the cytosolic tail from TFR…PromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
SFF-GFP
Plasmid#163657PurposeExpresses a TFR and ST6GAL1 swapped chimera with the cytosolic tail from ST6GAL1, and TMD and luminal domain from TFR in mammalian cellsDepositorInsertUseTagsGFPExpressionMammalianMutationThis chimera contains the cytosolic tail from ST,…PromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-MICU1-sh1-rORF
Plasmid#31764DepositorInsertMICU1 (MICU1 Human)
UsePdonr gatewayTagsNoneExpressionMutationRNAi-resistant ORF (rORF). 8 synonymous changes i…PromoterNoneAvailable sinceMay 4, 2012AvailabilityAcademic Institutions and Nonprofits only