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PurposeTET1-catalytic domain (CD) expression vector for TALE-TET1 fusion protein to induce targeted demethylation in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 49239 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneunknown
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Backbone manufacturerJoung lab
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Vector typeTALEN ; TALE-TET1 Expression Vector
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL1 Blue
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTET1 tet methylcytosine dioxygenase 1
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SpeciesH. sapiens (human), Synthetic
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Entrez GeneTET1 (a.k.a. CXXC6, LCX, bA119F7.1)
- Promoter EF1a
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer OMM520
- 3′ sequencing primer OJA199 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Location of plasmid features:
EF1alpha promoter = 922-2106
NLS = 2118-2142
TALE N-terminal domain = 2167-2577
Clone TALE array repeats between BsmBI sites
TALE C-terminal domain = 2665-2949
TET1 catalytic domain = 2956-5123
BGH polyA = 6369-6596
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJFA274 was a gift from Keith Joung (Addgene plasmid # 49239 ; http://n2t.net/addgene:49239 ; RRID:Addgene_49239) -
For your References section:
Targeted DNA demethylation and activation of endogenous genes using programmable TALE-TET1 fusion proteins. Maeder ML, Angstman JF, Richardson ME, Linder SJ, Cascio VM, Tsai SQ, Ho QH, Sander JD, Reyon D, Bernstein BE, Costello JF, Wilkinson MF, Joung JK. Nat Biotechnol. 2013 Oct 9. doi: 10.1038/nbt.2726. 10.1038/nbt.2726 PubMed 24108092