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Plasmid#181710PurposeGalactose inducible expression of hnRNPA1DepositorInserthnRNPA1 (HNRNPA1 Human)
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
GBH-R-cmlc2GFP.1
Plasmid#188004PurposeCloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafishDepositorInsertTargeted mRFP protein trap; secondary marker cmlc2 GFP
UseTargetable insertional mutagen and reporter systemTagsExpressionMutationPromoterEndogenuous Promoter; cmlc2 promotorAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pREMI-ZA
Plasmid#183736PurposePlasmid for a restriction enzyme mediated integration (REMI) in Komagataella phaffiiDepositorInsertSh ble
UseRandom insertional gene deletion in komagataella …TagsExpressionMutationPromoterTEF1 promoterAvailable sinceMarch 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
TRIPZ-CTID195-GSQ-UBnc-PURO
Plasmid#208037PurposeEnables inducible expression of C-terminal Split-TurboID-fused UBnc, to perform Ubiquitin-ID; nc = non-cleavable mutation; selection with puromycinDepositorInsertUbnc (UBC Human)
UseLentiviralTagsMyc, C-terminal Split-TurboIDExpressionMutationL73P Mutation near C-terminus suppresses cleavage…PromotertetO/UBCAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
TRIPZ-CTID195-GSQ-SUMO1nc-PURO
Plasmid#208035PurposeEnables inducible expression of C-terminal Split-TurboID-fused SUMO1nc, to perform SUMO-ID; nc = non-cleavable mutation; selection with puromycinDepositorInsertSUMO1nc (SUMO1 Human)
UseLentiviralTagsMyc, C-terminal Split-TurboIDExpressionMutationQ94P Mutation near C-terminus suppresses cleavage…PromotertetO/UBCAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-opto-hRIP3 dRHIM
Plasmid#208788PurposeInducibly expresses human RIP3, lacking RHIM domain, fused to light-activatable Cry2DepositorInsertRIP3 (RIPK3 Human)
UseLentiviralTagsCry2 mCherryExpressionMammalianMutationRHIM domain is removedPromoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVTHM-mSox2-3xflag
Plasmid#206376PurposeExpresses 3xflag fused mouse SOX2 in mammalian cells, for lentivirus generation.DepositorInsertSox2 (Sox2 Mouse)
UseLentiviralTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
GW1-ARSeNL
Plasmid#207873PurposeMammalian expression of the BRET ATP sensorDepositorInsertARSeNL
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
SWEET11:SWEET11-2A-GFP-GUS
Plasmid#172484PurposeTranslational fusion of SWEET11 with a P2A self cleaving peptide sequenceDepositorInsertSWEET11 (AT3G48740 Mustard Weed)
UseTagsGFP, GUSExpressionPlantMutationPromoterSWEET11Available sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAL250
Plasmid#201672PurposeContains 250 bp region of Pxyl promoter; insensitive to xylose.DepositorTypeEmpty backboneUseExpression in haloarchaeaTagsExpressionMutationPromoterPfdx, PxylAvailable sinceMay 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Bmpr2Δ751-813aa
Plasmid#163603PurposeExpresses BMPR-2 lacking the LIMK-binding region in mammalian cellsDepositorInsertBmpr2 (Bmpr2 Mouse)
UseTagsExpressionMammalianMutationThe LIMK-binding region (751-813 aa) is deletedPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJK_ToeRepG2_N19_trigger
Plasmid#132747PurposeT7 RNAP-driven expression of second-generation toehold repressor index 19 trigger RNADepositorInsertToeRepG2_N19_trigger
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR CMV Luc2 (FF-Luciferase) L5-L2
Plasmid#62168PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and firefly luciferase module. Compatible with MultiSite Gateway cloningDepositorInsertLuciferase
UseLuciferase; Mule gateway entry vectorTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
CN2109-rAAV-eHGT_410m-minBglobin-SYFP2-WPRE3-BGHpA
Plasmid#208157PurposeDirect-expressing SYFP2 in oligodendrocytes AAV VirusDepositorInsertSYFP2
UseAAVTagsExpressionMutationNAPromoterBeta Globin minimal promoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCXB-EBNA1
Plasmid#41857DepositorInsertEBNA-1 (EBNA-1 human herpesvirus 4)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV TRE3G Puro FLAG-DD-ER-mCherry-TRF1-FokI D450A
Plasmid#207953Purposenuclease inactive control for TRF1FokI WTDepositorInsertTRF1 (TERF1 Human)
UseLentiviralTagsFlag, DD, ER, mCherry and FokI (D450A)ExpressionMammalianMutationPromoterTRE3GAvailable sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-EF1alpha-VP64-dCas9-VP64_Blast
Plasmid#192650Purpose3rd generation lenti vector encoding VP64-dCas9-VP64 with 2A Blast resistance markerDepositorInsertVP64-dCas9-VP64
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A and N863A in Cas9PromoterEF1aAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
H2B-mCherry
Plasmid#20972PurposeH2B fused to mCherry for chromatin visualizationDepositorInsertHistone cluster 1, H2bb (H2BC3 Human)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceMay 7, 2009AvailabilityAcademic Institutions and Nonprofits only -
Lenti-multi-Guide
Plasmid#85401PurposeLentiviral vector for the delivery of multiple sgRNAs targeting different genes in combination with inducible Cas9 expresssion by pLenti-iCas9-neoDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-RUNX2
Plasmid#192926PurposeBarcoded piggybac transposon vector with Dox-inducible expression of RUNX2DepositorInsertRUNX2 (RUNX2 Human)
UseTagsHAExpressionMammalianMutationPromoterTET3G-miniCMVAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only