pLVTHM-mSox2-3xflag
(Plasmid
#206376)
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PurposeExpresses 3xflag fused mouse SOX2 in mammalian cells, for lentivirus generation.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 206376 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLVTHM-3xflag
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSox2
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SpeciesM. musculus (mouse)
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Entrez GeneSox2 (a.k.a. Sox-2, lcc, ysb)
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Tag
/ Fusion Protein
- 3xFLAG (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLVTHM-mSox2-3xflag was a gift from Ralf Jauch (Addgene plasmid # 206376 ; http://n2t.net/addgene:206376 ; RRID:Addgene_206376) -
For your References section:
Evaluation of the determinants for improved pluripotency induction and maintenance by engineered SOX17. Hu H, Ho DHH, Tan DS, MacCarthy CM, Yu CH, Weng M, Scholer HR, Jauch R. Nucleic Acids Res. 2023 Aug 23:gkad597. doi: 10.1093/nar/gkad597. 10.1093/nar/gkad597 PubMed 37607832