-
Plasmid#58220PurposeGreen fluorescent indicator for calcium imaging in the mitochondria (low affinity variant)DepositorInsertCEPIA4mt
UseTagsmycExpressionMammalianMutationGCaMP2 N263Y E282V E334D M339L F395W D436EPromoterCMVAvailable sinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
GFP-Rab27B
Plasmid#89447PurposeExpression of GFP-tagged human Rab27B in mammalian cellsDepositorInserthuman Rab27B (RAB27B Human)
UseTagseGFPExpressionMammalianMutationwild typePromoterCMVAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCas9-HE
Plasmid#109400PurposeExpresses Cas9 fusion to HE (HDR-enhancer) domain of human CtIP proteinDepositorInsertHDR-Enhancer domain of human CtIP protein (RBBP8 Human)
UseTagsExpressionMutationPromoterAvailable sinceJune 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
Fibronectin-human-plasma in pMAX
Plasmid#120401Purposeenables eukaryotic expression of human plasma fibronectinDepositorInsertFibronectin (FN1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pInducer10-mir-RUP-PheS
Plasmid#44011PurposeLentiviral shRNA vector (pGIPz based) for inducible knockdownDepositorInsertPheS Gly294
UseLentiviralTagsRFPExpressionMammalianMutationPromoterTRE2Available sinceJuly 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsEDC3
Plasmid#25032DepositorInsertEDC3 (EDC3 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceJune 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pSS9
Plasmid#71655PurposeContains 600 bp homology arms to SS9 for one-step markerless genome integration in E. coliDepositorInsertgfpuv
UseSynthetic BiologyTagsExpressionMutationPromoterT7A1Available sinceJan. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
PGKneotpAlox2
Plasmid#13444DepositorInsertPGKneotpAlox2
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pFLAG-Egfr(Velvet)
Plasmid#18788DepositorInsertepidermal growth factor receptor (Egfr Mouse)
UseTagsFLAGExpressionMammalianMutationD833G; signal sequence removed (aa1-24)PromoterAvailable sinceJuly 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pPM28 (eroGFP CEN/ARS URA3)
Plasmid#20131DepositorInserteroGFP (KAR2 jellyfish, Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 11, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSLQ1658-dCas9-EGFP
Plasmid#51023PurposeTemplate for NLS-dCas9-NLS-EGFP fusion protein for CRISPR imaging (the recipient vector can be TetON 3G promoter system)DepositorInsertdCas9 fuse to EGFP
UseCRISPR and RetroviralTagsEGFPExpressionMammalianMutationPromoterMSCV LTR promoterAvailable sinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pgRNA-CKB
Plasmid#73501PurposegRNA expression vector (with mKate2)DepositorInsertmKate2-T2A-Bsd
UseCRISPR and LentiviralTagsNLSExpressionMammalianMutationPromoterCAGAvailable sinceDec. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
hWIP1 FLAG
Plasmid#28105DepositorInsertWIP1 (PPM1D Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceApril 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRcCMV myc-Plk1 T210D (Nigg LA183)
Plasmid#41158DepositorInsertPlk1 (PLK1 Human)
UseTagsMycExpressionMammalianMutationT210DPromoterCMVAvailable sinceFeb. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMSCVpuro-eGFP-hcGAS
Plasmid#108674PurposeFor production of retrovirus and transduction of mammalian cells, allowing expression of N-terminally eGFP-tagged human cGASDepositorInserteGFP-cGAS (CGAS Human)
UseRetroviralTagsExpressionMutationPromoterLTRAvailable sinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE-3
Plasmid#72678PurposeExpresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Kan resistance marker.DepositorInsertmutL E32K
UseTagsnoneExpressionBacterialMutationE32K mutation conferring dominant mutator phenoty…PromoterAvailable sinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
IL-2 promoter luciferase
Plasmid#12194DepositorInsertIL-2 promoter (Il2 Mouse)
UseLuciferaseTagsluciferaseExpressionBacterialMutationPromoterAvailable sinceJuly 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
pHRIG-Akt1
Plasmid#53583Purposelentiviral expression of constitutively active human Akt1 and EGFPDepositorInsertcAkt1 (AKT1 Human)
UseLentiviralTagsHA and IRES-EGFPExpressionMammalianMutationconstitutively active (myristoylated version)PromoterCMVAvailable sinceOct. 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pC0050-CMV-dPspCas13b-longlinker-ADAR2DD(wt)
Plasmid#103866PurposedPspCas13b-ADAR2DD(wt) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA. Longer linker than pC0039.DepositorInsertsUseCRISPRTagsGSGGGGS and HIV NESExpressionMammalianMutationChanged histidne 133 to alanine and histidine 105…PromoterAvailable sinceNov. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
Syn-ATP
Plasmid#51819PurposeOptical reporter of presynaptic ATPDepositorInsertChimeric Synaptophysin-mCherry-Luciferase
UseTagsExpressionMammalianMutationWithin WT luciferase gene, changed Threonine 214 …PromoterCMVAvailable sinceMarch 13, 2014AvailabilityAcademic Institutions and Nonprofits only