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Plasmid#25965DepositorInsertB-Myb (MYBL2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBABE GFP
Plasmid#10668PurposeEmpty backbone for retroviral gene expression. Select recipient cells using GFP.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2005AvailabilityAcademic Institutions and Nonprofits only -
Lenti_sgRNA(MS2)_MCP-VP64-IRES-zsGreen1
Plasmid#138461Purposelenti sgRNA cloning backbone with MS2 loops and EF1a-MCP-VP64DepositorInsertMCP-VP64-IRES-zsGreen1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1AAvailable sinceMay 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
GRM5-DuET
Plasmid#213303PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertGRM5 (GRM5 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Sport6-CD9-pHuji
Plasmid#130906PurposeExpression of CD9-pHuji for visualization of multivesicular body-plasma membrane fusion.DepositorInsertCD9-pHuji (CD9 Human, Synthetic)
UseTagspHujiExpressionMammalianMutationpHuji inserted between Asn50 and Asn51 in extrace…PromoterCMVAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-TO-oNGN2
Plasmid#198397PurposePiggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase, NGN2 optimization aided by IDT tool.InsertsUseTagsT2A-mycNLS-mTagBFP2ExpressionMammalianMutationS24A, S193A, S207A, S209A, S219A, S232A, S239A, S…PromoterCAG, EF1a, and TRE3GAvailable sinceApril 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
Flag-FOXO3
Plasmid#153142PurposeExpression of Flag tagged human FOXO3 in mammalian cellsDepositorInserthuman FOXO3 (FOXO3 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMV IE94Available sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
tet_pLKO.1_puro_shNRF2 #1
Plasmid#136584PurposeExpresses an inducible short hairpin targeting human NRF2 sequenceDepositorInsertshNRF2v1 (NFE2L2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6.2 EmGFP hAQP4(M23)
Plasmid#126464PurposeExpresses human AQP4 (isoform M23) as an EmGFP fusion protein in mammalian cellsDepositorInsertAquaporin 4 (AQP4 Human)
UseTagsEmGFPExpressionMammalianMutationPromoterAvailable sinceMay 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-BirA-V5-neo
Plasmid#100548PurposeExpresses C-terminal V5-tagged BirA biotin ligaseDepositorInsertC-terminal V5-tagged BirA
UseTagsV5, HisExpressionMammalianMutationPromoterHuman EF1aAvailable sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
lentiSAM v2 (Puro)
Plasmid#92062PurposeModified version of lentiSAM v2, a lenti sgRNA cloning backbone with MS2 loops at tetraloop/stemloop 2, dCas9-VP64, and puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 (sgRNA) and EF1a (dCas9-VP64)Available sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA5/FRT/TO DNAJB1
Plasmid#19468DepositorInsertDNAJB1 (DNAJB1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
Cilantro 2
Plasmid#74450PurposeCan clone in a C2H2 zinc finger via BsmBI restriction sites and monitor post-translational degradation using EGFP:mCherry ratio (Lentiviral, PGK.BsmBICloneSite-EGFP.IRES.mCherry.cppt.EF1a.PuroR)DepositorTypeEmpty backboneUseLentiviralTagsEGFPExpressionMutationPromoterAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
EP-Pol vector
Plasmid#131686PurposeA lentiviral vector that constitutively expresses an error-prone variant of the adenovirus DNA polymeraseDepositorInsertAdenovirus DNA polymerase
UseLentiviralTagsHAExpressionMutationF421Y, D827APromoterAvailable sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSCV mKeima-LC3
Plasmid#189006PurposeFluorescent reporter for autophagyDepositorInsertmKeima, LC3 (Map1lc3b Rat)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
CAG-FR-GECO1c
Plasmid#163682PurposeFar-red fluorescent calcium indicator FR-GECO1cDepositorInsertFR-GECO1c
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFX-mito-EGFP
Plasmid#159096PurposeExpress EGFP with mitochondria-targeting sequence in mammalian cellsDepositorInsertmCherry with mitochondria-targeting sequence
UseTagsthe mitochondria-targeting sequence of the cytoch…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMV-GFP-NMHC II-A
Plasmid#11347DepositorInsertNMHC II-A (MYH9 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLenti Dest BlastR R1-R2
Plasmid#84574PurposeGateway destination vector expressing Blasticidin resistance under a PGK promoterDepositorTypeEmpty backboneUseLentiviral; GatewayTagsExpressionMammalianMutationPromoterPGKAvailable sinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
APOBEC3G_pLX307
Plasmid#98345PurposeLentiviral expression of APOBEC3GDepositorInsertAPOBEC3G (APOBEC3G Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterE1FaAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAcGFP1-mito
Plasmid#174180PurposeExpress AcGFP1 with mitochondria-targeting sequence in mammalian cellsDepositorInsertAcGFP with mitochondria-targeting sequence
UseTagsthe mitochondria-targeting sequence of the cytoch…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT3-EF1A-MYC-IRES-lucOS
Plasmid#129776PurposeTransposon based vector that expresses human MYC followed by IRES and firefly luciferase and the antigens SIYRYYGL (SIY), SIINFEKL (SIN, OVA257-264), and OVA323-339DepositorInserthuman MYC (MYC Human)
UseTagsOS: SIYRYYGL (SIY), SIINFEKL (SIN, OVA257-264), a…ExpressionMammalianMutationPromoterEF1AAvailable sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
330-Plakoglobin-Myc
Plasmid#32228DepositorInsertPlakoglobin (JUP Human)
UseTagsMycExpressionMammalianMutationPromoterhuman beta-actinAvailable sinceOct. 26, 2011AvailabilityAcademic Institutions and Nonprofits only -
pOpen-SP6rpol
Plasmid#165516PurposeDNA-dependent RNA polymerase derived from SP6 bacteriophage that catalyzes the transcription of viral genes.DepositorInsertSP6 RNA Polymerase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 29, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-CaMKIIa-HA-hM3D(Gq)-IRES-mCitrine
Plasmid#50466PurposeGq-coupled hM3D-IRES-mCitrine under the control of CaMKIIa promoterDepositorInserthM3D(Gq)-IRES-mCitrine (CHRM3 Human)
UseAAVTagsHAExpressionMutationSee supplemental documents for DREADD mutationsPromoterCaMKIIaAvailable sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2 TDP-43 5F-L YFP
Plasmid#84914PurposeMammalian expresion of TDP-43 5F-L YFPDepositorInsertTDP-43 (TARDBP Human)
UseTagsYFPExpressionMammalianMutationF147L, F149L, F194L, F229L, F231LPromoterCMVAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pT7C-HSPA8
Plasmid#177661PurposeRecombinant protein synthesis of human HSPA8DepositorInsertHSPA8 (HSPA8 Human)
UseTags6xHis-ThrombinExpressionBacterialMutationPromoterT7Available sinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
FLAG-VAMP7
Plasmid#45913DepositorInsertvesicle-associated membrane protein 7 (Vamp7 Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
hTLR2 flag
Plasmid#13082DepositorInsertTLR2 (TLR2 Human)
UseTagsFlagExpressionMammalianMutationAmino acids 1-16 have been replaced with mammalia…PromoterAvailable sinceOct. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCAG-VSVN
Plasmid#64087PurposeExpression vector for Vesicular Stomatitis Virus Indiana strain nucleoprotein geneDepositorInsertVSV nucleoprotein
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTW084b
Plasmid#116894PurposeActivity-independent SE-PACE APDepositorInsertsg3N
434-cI/scFv m3
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG_smFP V5
Plasmid#59758Purposeencodes multiple copies of an epitope tag V5DepositorInsertsmFP _V5
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceSept. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
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NFAT/AP-1 3x luciferase
Plasmid#11783DepositorInsertNFAT/AP-1 3x (Il2 Mouse)
UseLuciferaseTagsluciferaseExpressionBacterialMutationPromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
FRP2350
Plasmid#127580PurposeTagging plasmid for WTC 846: Encodes the PtetO7.1 promoter and Hyg resistance geneDepositorInsertPtetO7.1_Flagtag_8Glycine linker
UseSynthetic Biology; Pcr based taggingTagsFlag tag, 8glycine linkerExpressionMutationPromoterAvailable sinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
GST-FOXO3a WT
Plasmid#1790DepositorInsertFOXO3a (FOXO3 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceDec. 8, 2005AvailabilityAcademic Institutions and Nonprofits only -
pMKV059
Plasmid#133314PurposeBeYDV viral replicon on T-DNA backbone expressing Firefly Luc+ and IPTDepositorInsertLuc+, IPT
UseTagsExpressionPlantMutationPromoterAtUbi10, 35SAvailable sinceJan. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine
Plasmid#50467PurposeGi-coupled hM4D-IRES-mCitrine under the control of CaMKIIa promoterDepositorUseAAVTagsHAExpressionMutationSee supplemental documents for DREADD mutationsPromoterCaMKIIaAvailable sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCK002_U6-Sa-sgRNA(mod)_EFS-SaCas9-2A-Puro_WPRE
Plasmid#85452PurposeLentiviral vector encoding modified SaCas9 system backbone bearing BsmBI site for new guide RNAs and puromycin selection marker.DepositorInsertU6-modified-sgRNA-Backbone-EFS-hSaCas9-2xNLS-2A-Puro
UseCRISPR and LentiviralTagsNLSExpressionMammalianMutationPromoterAvailable sinceApril 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-StagRFP
Plasmid#173014PurposeRationally enhanced red fluorescent protein derived from TagRFP-T. For mammalian expression.DepositorInsertStagRFP
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCGN N-Ras wt
Plasmid#14723DepositorInsertN-ras (NRAS Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceAug. 29, 2007AvailabilityAcademic Institutions and Nonprofits only -
HA-HIF1alpha P402A/P564A-pcDNA3
Plasmid#18955DepositorInsertHypoxia inducible factor 1 alpha (HIF1A Human)
UseTagsHA-tagExpressionMammalianMutationcDNA changed amino acids: Proline 402 to Alanin…PromoterAvailable sinceAug. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 GFP LIC cloning vector (6D)
Plasmid#30127DepositorTypeEmpty backboneUseTagsGFPExpressionMammalianMutationPromoterAvailable sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET_mNG2(1-10)_32aalinker_mNG2(11)
Plasmid#82611PurposeBacterial expression vector for split mNeonGreen2 screeningDepositorInsertmNeonGreen2(1-10)_32aalinker_mNeonGreen2(11)
UseTagsExpressionBacterialMutationsplit before mutationsPromoterT7Available sinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
TFORF1983
Plasmid#143562PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTBX5 (TBX5 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMi(3xP3-EGFP)
Plasmid#102540PurposeTransgenesis vector based on the Minos transposon, carrying the 3xP3-EGFP markerDepositorInsertMinos transpoable element with 3xP3-EGFP marker
UseTagsExpressionMutationPromoterAvailable sinceMarch 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBW2661_pCAG-PV1-iCre-N270-L1-ABI-NLS-BGHpA
Plasmid#108745PurposeExpresses N-terminus to aa270 of Cre recombinase fused to ABI CIDDepositorInsertCre recombinase fragment N-terminus to aa270
UseCre/Lox and Synthetic BiologyTagsABI,NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
phage UbiC NLS HA stdMCP stdHalo
Plasmid#104999PurposeLentiviral vector expressing synonymous tandem MS2 coat protein fused to synonymous tandem Halo-tag for mRNA labelingDepositorInsertstdMCP-stdHalo
UseLentiviralTagsHA tag, NLS, Two synonymous copies of the Halo-ta…ExpressionBacterial and MammalianMutationPromoterUbiCAvailable sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
Pet28a_Human_H3.1
Plasmid#42631DepositorInsertHuman H3.1 (H3C1 Human)
UseTagsUntaggedExpressionBacterialMutationPromoterT7Available sinceFeb. 25, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits