Skip to main content
Addgene
Showing: 8101 - 8150 of 156611 results

  4. pET-19b_MMLV-RT

    Plasmid
    #153312
    Purpose
    Bacterial expression of MMLV reverse transcriptase
    Depositor
    Drew Endy, Philippa Marrack
    Insert
    MMLV Reverse Transcriptase
    Use
    Tags
    10x His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 2, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153312-plasmid-map-sequence-id-298742

    Enlarge

  10. PP-GSK3

    Plasmid
    #25133
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    cgd4_240:C29838
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 5, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  16. pSKI

    Plasmid
    #232484
    Purpose
    pSKI can be used for single-copy transgene expression within the Universal SKI System or for extrachromosomal arrays. It contains an MCS with 47 single cuts and two synthetic 900 bp homology arms.
    Depositor
    Carlos Silva García
    Article
    Dinneen et al bioRxiv [Preprint]. 2024 Dec 10:2024.12.
    Insert
    pSKI
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    232484-plasmid-map-sequence-id-464988

    Enlarge

  18. p2T-CMV-ABE8e_dNRCH-BlastR

    Plasmid
    #232720
    Purpose
    A-to-G base editor
    Depositor
    David Liu
    Insert
    ABE8e-dCas9(NRCH)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    within Cas9 D10A H840A
    Promoter
    Available since
    March 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    232720-plasmid-map-sequence-id-463544

    Enlarge

  19. TFORF1201

    Plasmid
    #142751
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    SETDB1 (SETDB1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    142751-plasmid-map-sequence-id-462894

    Enlarge

  23. miniTurbo-dCasRx

    Plasmid
    #219821
    Purpose
    EF1α-driven expression of miniTurbo fused to dCasRx with GFP (Adapted from plasmid #153209)
    Depositor
    Philipp Maass
    Insert
    dCasRx
    Use
    CRISPR
    Tags
    miniTurbo
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a and hU6
    Available since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    219821-plasmid-map-sequence-id-440859

    Enlarge

  25. L1T10 - T2PB/pUBQ10 (mTagBFP2, mCherry, NeonGreen)

    Plasmid
    #219687
    Purpose
    Level 1 History-dependent target, switch PhiC31 then Bxb1, pUBQ10 promoter, and output: mtagBFP2 then mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).
    Depositor
    Jennifer Nemhauser
    Insert
    T2PB/pUBQ10 (mTagBFP2, mCherry, NeonGreen)
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    219687-plasmid-map-sequence-id-435722

    Enlarge

  34. TFORF1508

    Plasmid
    #143817
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    MTF1 (MTF1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    143817-plasmid-map-sequence-id-396297

    Enlarge

  39. pVITRO1-102.1F10-IgM/λ

    Plasmid
    #61880
    Purpose
    Expresses grass pollen allergen Phl p 7 specific human IgM/λ antibody isotype (102.1F10-IgM/λ)
    Depositor
    Andrew Beavil
    Article
    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103
    Inserts
    grass pollen allergen Phl p 7 specific human Mu heavy chain expression cassette
    grass pollen allergen Phl p 7 specific human lambda light chain expression cassette
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    mEF1 Prom and rEF1 Prom
    Available since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    61880-plasmid-map-sequence-id-101189

    Enlarge

  40. PB-CT3G-CERP2-MG

    Plasmid
    #175503
    Purpose
    All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements (CMVe-hEF1a-driven rtTA and puromycin resistance)
    Depositor
    George Church
    Article
    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G-mCMV
    Available since
    Oct. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    175503-plasmid-map-sequence-id-345096

    Enlarge

  44. pgRNA-humanized

    Plasmid
    #44248
    Purpose
    Expression of customizable guide RNA (gRNA) under cotnrol of murine U6 promoter, also contains a CMV-puro-t2A-mCherry expression cassette for mammalian gene knockdown
    Depositor
    Stanley Qi
    Article
    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1
    Insert
    guide RNA
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    mouse U6
    Available since
    April 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    44248-plasmid-map-sequence-id-190219

    Enlarge

Showing: 8101 - 8150 of 156611 results