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Plasmid#210580PurposeExpresses SM(FLAG)-nonoptimal Firefly - 3'UTR malat1 triple helixDepositorInsertSpaghetti monster (FLAG)-nonoptimal FLuc - 3'UTR malat1 triple helix
UseLuciferaseTagsSpaghetti monster (FLAG)ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
Zhou-pLOV-GFP-Luc
Plasmid#161030PurposeLentiviral vector expressing GFP and luciferase for use as a reporter in pseudovirus production and infection.DepositorInsertGFP and luciferase
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAN007
Plasmid#220048PurposeReporter plasmid that encodes for the GFP fused to an N-terminal flag-HA epitope. A stop codon is inserted between the epitope tag and the gfp sequence to terminate GFP translation.DepositorInsertflag-HA-stop-GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Neo-LUC
Plasmid#214368PurposeExpression of the firefly luciferase ProteinDepositorInsertLUC
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)GαoA-91-NLuc
Plasmid#207151PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes transcript variant A of GNAO tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorInsertGNAO
UseTagsExpressionMammalianMutationNLuc inserted at position L(91)GPromoterAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)GαoB-91-NLuc
Plasmid#207152PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes transcript variant B of GNAO tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorInsertGNAO
UseTagsExpressionMammalianMutationNLuc inserted at position L(91)GPromoterAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL4.11_Nued2_E1_dAP1-2
Plasmid#203837PurposeLuciferase reporter plasmid for Igf1 enhancer 1 with both AP-1 sites knocked outDepositorInsertdAP1-2
UseTagsExpressionMammalianMutationKO of both AP1 sitesPromoterAvailable sinceOct. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
FB395
Plasmid#203631PurposeTU for the expression of luciferase under a synthetic promoter containing the target sequence for gRNA1 (1xLuc).DepositorInsertR1:G1aG2b.1:mPAF:Luc:TtrpC
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
FB396
Plasmid#203632PurposeTU for the expression of luciferase under a synthetic promoter containing two copies of the target sequence for gRNA1 (2xLuc).DepositorInsertR1:G1ab.1:mPAF:Luc:TtrpC
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
FB397
Plasmid#203633PurposeTU for the expression of luciferase under a synthetic promoter containing three copies of the target sequence for gRNA1 (3xLuc).DepositorInsertR1:G1abc.3:mPAF:Luc:TtrpC
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
FB312
Plasmid#203569PurposeTU for the expression of Nanoluciferase (Nluc) under the PgpdA promoter.DepositorInsertPgpdA:Nluc:Ttub
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL4.11_Nued2_E1_dAP1
Plasmid#203835PurposeLuciferase reporter plasmid for Igf1 enhancer 1 containing only the downstream AP1 siteDepositorInsertdAP1
UseTagsExpressionMammalianMutationKO of AP1 upstreamPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL4.11_Nued2_E1_dAP2
Plasmid#203836PurposeLuciferase reporter plasmid for Igf1 enhancer 1 containing only the upstream AP1 siteDepositorInsertdAP1
UseTagsExpressionMammalianMutationKO of AP1 downstreamPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMC-6-ELuc-9
Plasmid#197736PurposePhytobrick (MoClo) Level 0 PartDepositorInsertEmerald/Enhanced Click Beetle Luciferase (ELuc) (tomato codon-optimized)
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Camk2a
Plasmid#186818PurposeFluorescent reporter for Camk2a expressionDepositorInsertCamk2a promoter (Camk2a Mouse)
UseLuciferaseTagsExpressionMutationPromoterCamk2a promoterAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
shRNA-Ratio-NegCon
Plasmid#183554PurposeDesigned to test the efficacy of shRNA by assessing the ratio of GFP-fusion protein to RFP (shRNA ratio negative control (Luciferase), Fig S2)DepositorInsertLuciferase shRNA
UseTagsExpressionMammalianMutationPromoterCBhAvailable sinceJune 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVH608-Tier1-PmPGK1-Fluc
Plasmid#169543PurposeTier-1 vector encoding PmPGK1-driven Fluc (PmPGK1-Fluc-pA).DepositorInsertPPGK-driven Firefly Luciferase
UseTagsExpressionMammalianMutationPromoterPPGKAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
MtMscL delta49
Plasmid#118342PurposeM. tuberculosis MscL (delta49) in pET19bDepositorInsertMscL (mscL Mycobacterium tuberculosis)
UseTags6x His Tag and Thrombin Cleavage siteExpressionBacterialMutationChanged Glutamic Acid (Glu) 102 to Stop CodonPromoterT7 PromoterAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSI-check2-Cul1a-3'UTR
Plasmid#97152PurposeFor dual luciferase report assayDepositorInsertCul1a 3'UTR
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSI-check2-Cul1b-3'UTR
Plasmid#97153PurposeFor dual luciferase report assayDepositorInsertCul1b 3'UTR
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only