MtMscL delta49
(Plasmid
#118342)
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PurposeM. tuberculosis MscL (delta49) in pET19b
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 118342 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET19b
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5856
- Total vector size (bp) 6222
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMscL
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Alt namelarge-conductance ion mechanosensitive channel
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SpeciesMycobacterium tuberculosis
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Insert Size (bp)306
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MutationChanged Glutamic Acid (Glu) 102 to Stop Codon
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GenBank ID885368
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Entrez GenemscL (a.k.a. Rv0985c)
- Promoter T7 Promoter
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Tags
/ Fusion Proteins
- 6x His Tag (N terminal on insert)
- Thrombin Cleavage site (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7 Promoter
- 3′ sequencing primer T7 Reverse (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MtMscL delta49 was a gift from Douglas Rees (Addgene plasmid # 118342 ; http://n2t.net/addgene:118342 ; RRID:Addgene_118342) -
For your References section:
Elucidating a role for the cytoplasmic domain in the Mycobacterium tuberculosis mechanosensitive channel of large conductance. Herrera N, Maksaev G, Haswell ES, Rees DC. Sci Rep. 2018 Oct 1;8(1):14566. doi: 10.1038/s41598-018-32536-6. 10.1038/s41598-018-32536-6 PubMed 30275500