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Addgene

MtMscL delta49
(Plasmid #118342)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 118342 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pET19b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5856
  • Total vector size (bp) 6222
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MscL
  • Alt name
    large-conductance ion mechanosensitive channel
  • Species
    Mycobacterium tuberculosis
  • Insert Size (bp)
    306
  • Mutation
    Changed Glutamic Acid (Glu) 102 to Stop Codon
  • GenBank ID
    885368
  • Entrez Gene
    mscL (a.k.a. Rv0985c)
  • Promoter T7 Promoter
  • Tags / Fusion Proteins
    • 6x His Tag (N terminal on insert)
    • Thrombin Cleavage site (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MtMscL delta49 was a gift from Douglas Rees (Addgene plasmid # 118342 ; http://n2t.net/addgene:118342 ; RRID:Addgene_118342)
  • For your References section:

    Elucidating a role for the cytoplasmic domain in the Mycobacterium tuberculosis mechanosensitive channel of large conductance. Herrera N, Maksaev G, Haswell ES, Rees DC. Sci Rep. 2018 Oct 1;8(1):14566. doi: 10.1038/s41598-018-32536-6. 10.1038/s41598-018-32536-6 PubMed 30275500