Addgene

156,554 results

Showing: 6361 - 6400 of 156554 results

Results per page:

Sort results by:

4xNLS-pMJ915v2

Plasmid

#88917

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONRCBX

Plasmid

#29634

Depositor

David_L Andrews, Scott Gold

Article

Garcia-Pedrajas et al Fungal Genet Biol. 2008 Apr . 45(4):379-

Type

Empty backbone

Use

Gateway donor

Tags

Expression

Mutation

Promoter

Available since

Sept. 19, 2011

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pENN.AAV.cTNT.PI.eGFP.WPRE.rBG

Plasmid

#105543

Purpose

AAV expression of EGFP from cTNT promoter

Depositor

James M. Wilson

Has Service

AAV9

Insert

cTNT-PI-EGFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

cTNT

Available since

Feb. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
TGFB1_pLX307

Plasmid

#98377

Purpose

Lentiviral expression of TGFB1

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

TGFB1 (TGFB1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

E1Fa

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG654 pFAST

Plasmid

#172861

Purpose

Expresses pFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

Insert

pFAST

Use

Tags

Myc tag (C terminal on insert)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
NG-ABEmax

Plasmid

#124163

Purpose

A-to-G base editor

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

Insert

TadA-TadA(evo)-Cas9-NG

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiT3.1-N_FBXW7:1-707

Plasmid

#211099

Purpose

Mammalian expression of full-length human FBXW7. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

FBXW7:M1-K707 (FBXW7 Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
U6GRNA

Plasmid

#68370

Purpose

gRNA scaffold with hU6 promoter

Depositor

Eric-Paul Bennett

Article

Lonowski et al Nat Protoc. 2017 Mar;12(3):581-603. doi:

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS Omomyc

Plasmid

#113164

Purpose

Omomyc expression in mammalian cells

Depositor

Sergio Nasi

Article

Soucek et al Oncogene. 1998 Nov 12;17(19):2463-72. do

Insert

Omomyc (MYC Human)

Use

Tags

Expression

Mammalian

Mutation

E57T, E64I, R70Q, R71N

Promoter

Available since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti6/V5-DEST-NCAPH

Plasmid

#31214

Depositor

Lynda Chin

Article

Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

Insert

NCAPH (NCAPH Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

July 29, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-Myc-IQGAP1

Plasmid

#30118

Depositor

David Sacks

Article

Ho et al J Biol Chem. 1999 Jan 1. 274(1):464-70.

Insert

IQGAP1 (IQGAP1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 17, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
mEmerald-TGNP-N-10

Plasmid

#54279

Purpose

Localization: Golgi Complex, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

TGNP (TGOLN2 Human)

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pAAV-hsyn-NE2m

Plasmid

#208686

Purpose

Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2m in neurons

Depositor

Yulong Li

Insert

GPCR activation based norepinephrine (NE) sensor GRAB_NE2m

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
PAmCherry1-N1-Tubbyc

Plasmid

#202677

Purpose

Photoactivatable lipid biosensor

Depositor

Gerry Hammond

Article

Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

Insert

Mus musculus Tub (243-505): PAmCherry1 (Tub Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pKLV2-U6gRNA5(gBFP)-PGKmCherry2ABFP-W

Plasmid

#67986

Purpose

Cas9 activity reporter with mCherry and BFP

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Inserts

U6gRNA cassette, PGKmCherryABFP cassette, WPRE
Guide RNA targeting modified BFP

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Deleted BbsI site within WPRE, modified BFP

Promoter

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMAZ-SK

Plasmid

#73962

Purpose

self-killing gRNA plasmid pMAZ-SK

Depositor

Alex Nielsen

Article

Ronda et al Sci Rep. 2016 Jan 22;6:19452. doi: 10.10

Insert

gRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

pTet

Available since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pBA560

Plasmid

#186236

Purpose

pTet-eLbuCas13a crRNA entry vector

Depositor

Jennifer Doudna

Article

Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-Rab5CA(Q79L)

Plasmid

#35138

Depositor

Sergio Grinstein

Article

Bohdanowicz et al Mol Biol Cell. 2012 Jan;23(1):176-87. Ep

Insert

Rab5a (RAB5A Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Constitutively Active- Q79L

Promoter

CMV

Available since

March 20, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-TAC-OSKM

Plasmid

#80481

Purpose

piggyBac transposon for dox-inducible expression of the OSKM polycistronic reprogramming cassette (with mCherry)

Depositor

Knut Woltjen

Article

Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

Insert

OSKM (Pou5f1, Sox2, Klf4, Myc Mouse)

Use

Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

tetO

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-4T-1-3xHA

Plasmid

#129567

Purpose

Empty backbone for HA tag

Depositor

Kevin Janes

Article

Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSR1038

Plasmid

#208737

Purpose

Characterization plasmid for PCap (sfgfp expressed from PCap on the S. aureus pC194 replicon)

Depositor

Lauren Andrews

Article

Rondthaler et al ACS Synth Biol. 2023 Dec 8. doi: 10.1021

Insert

PCap

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PCap

Available since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-TDP-43

Plasmid

#190093

Purpose

Expresses TDP-43 in mammalian cells. Note: EGFP and TDP-43 are expressed in different reading frames.

Depositor

Bowen Liu

Article

Liu et al Commun Biol. 2022 Aug 13;5(1):816. doi:

Insert

TAR DNA-Binding Protein 43 (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
SIRT6 Flag

Plasmid

#13817

Purpose

Mammalian expression of human SIRT6 with flag tag

Depositor

Eric Verdin

Article

North et al Mol Cell. 2003 Feb . 11(2):437-44.

Insert

SIRT6 (SIRT6 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
TOP-GFP.mC

Plasmid

#35491

Depositor

Ramesh Shivdasani

Article

Horst et al Cancer Res. 2012 Feb 8.

Insert

7xTOP-GFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

7xTCF/LEF

Available since

May 15, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pIRES-Ap2a2-FKBP

Plasmid

#46944

Purpose

Co-expresses siRNA-resistant, FKBP-tagged AP-2 alpha subunit and G418 resistance gene in mammalian cells

Depositor

Margaret Robinson

Article

Robinson et al Dev Cell. 2010 Feb 16;18(2):324-31. doi:

Insert

Ap2a2-FKBP

Use

Tags

Mainly mouse Ap2a2 cDNA, with a fragment of brain…

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 23, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-C1-PKM2

Plasmid

#64698

Purpose

Expression of GFP tagged PKM2 in mammalian cells

Depositor

Axel Ullrich

Article

Stetak et al Cancer Res. 2007 Feb 15;67(4):1602-8.

Insert

PKM2 (PKM Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pTK229

Plasmid

#71256

Purpose

luciferase reposter with 229 bp of the HSV TK promoter

Depositor

Peter Cockerill

Article

Hawwari et al J Immunol. 2002 Aug 15;169(4):1876-86.

Insert

Thymidine Kinase promoter

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

HSV Thymidine Kinase

Available since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
FUGW-Mito-TurboID

Plasmid

#200963

Purpose

3rd gen lentiviral plasmid for expressing mitochondria localized TurboID with EGFP

Depositor

Keriann Backus

Article

Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

Insert

Mito-TurboID

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEMHE-H2B-mEGFP

Plasmid

#105528

Purpose

T7 promotor drives in vitro transcription of mEGFP-tagged Histon 2B mRNA

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

H2B (H2BC11 Human)

Use

In vitro transcription

Tags

mEGFP

Expression

Mutation

Promoter

T7

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
HO-hisG-URA3-hisG-poly-HO

Plasmid

#51663

Purpose

yeast plasmid for integration of target sequence at HO locus, URA3 selection

Depositor

David Stillman

Article

Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E5

Type

Empty backbone

Use

Yeast integration

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-NPM1wt-IRES-mCherry

Plasmid

#131824

Purpose

Lentiviral plasmid expressing NPM1 wildtype (human) in mammalian cells upon transduction

Depositor

Mark Minden

Article

Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

Insert

NPM1 (NPM1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
p667-UBC-CDC42BPA-V5-miniTurbo_IDG-K

Plasmid

#189907

Purpose

Gateway destination clone of CDC42BPA (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

CDC42BPA (CDC42BPA Human)

Use

Lentiviral; Gateway destination

Tags

V5-miniTurbo

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDM4

Plasmid

#49796

Purpose

ePathBrick vector for pathway engineering in E. coli.

Depositor

Mattheos Koffas

Article

Xu et al ACS Synth Biol. 2012 Jul 20;1(7):256-66.

Type

Empty backbone

Use

Tags

S-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 31, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pLminP_Luc2P_RE14

Plasmid

#90349

Purpose

VDR - Vitamin D receptor gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Tags

Expression

Mutation

Promoter

VDR

Available since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-MDFIC

Plasmid

#207835

Purpose

Tagged MDFIC for western blotting and imaging

Depositor

Charles Cox

Article

Zhou et al Science. 2023 Aug 18;381(6659):799-804.

Insert

MDFIC (MDFIC Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAF0689 Ef1a-Pa01_attB-BFP acceptor

Plasmid

#193472

Purpose

Acceptor plasmid for 3 plasmid recombination assay

Depositor

Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Pa01_attB

Use

Tags

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-Peredox-mCherry

Plasmid

#32383

Depositor

Gary Yellen

Article

Hung et al Cell Metab. 2011 Oct 5;14(4):545-54.

Insert

Peredox-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pLminP_Luc2P_RE45

Plasmid

#90383

Purpose

EGR1 - Early growth response factor gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Tags

Expression

Mutation

Promoter

GLI

Available since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBITE-EGFRvIII-F269-OKT3

Plasmid

#190681

Purpose

Encodes an EGFRvIII-CD3 BITE protein (F269 clone)

Depositor

Scott McComb

Article

Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

Insert

Anti-EGFRvIII-scFv-F269-modularized-OKT3-HisTag

Use

Lentiviral

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_BMP2_WT

Plasmid

#82101

Purpose

Gateway Donor vector containing BMP2 , part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, David Root, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

BMP2 (BMP2 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 6361 - 6400 of 156554 results

Results per page:

Sort results by:

	More than 20 requests
	More than 50 requests
	More than 100 requests