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Plasmid#199255PurposeExpression construct encoding the high specificity eSpCas9(1.1) nucleaseDepositorInsertHigh specificity eSpCas9(1.1) nuclease
UseCRISPRTags3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianMutationK848A K1003A R1060APromoterCAG promoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET30b_T7Sav
Plasmid#138588PurposeT7-tagged streptavidin with native C-terminus controlled by PT7 promoter; KanR, pBR322 oriDepositorInsertT7-tagged streptavidin variant with native C-terminus
UseSynthetic BiologyTagsT7-tagExpressionBacterialMutationPromoterPT7Available sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pRL25
Plasmid#70253PurposeShuttle vector suitable for transfer to Anabaena (Nostoc) sp. strain PCC 7120 and other cyanobacterial strainsDepositorTypeEmpty backboneUseBacterial shuttle vectorTagsExpressionMutationPromoterAvailable sinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
AB65_pCAG.Cas9-D10A.rBGpA
Plasmid#199256PurposeExpression construct encoding the SpCas9-D10A nickaseDepositorInsertSpCas9.D10A nickase
UseCRISPRTagsSV40 NLSExpressionMammalianMutationD10APromoterCAG promoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAL3
Plasmid#114673PurposeT7 vector having very low basal expression of target protein can clone and express almost any gene. Asymmetric ligation clones as many as 3 genes simultaneously for co-expression from the same mRNA.DepositorTypeEmpty backboneUseTagsoptional Ser-Gly-6HisExpressionBacterialMutationPromoterT7Available sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRnc-gGFP161
Plasmid#189536PurposeExpresses E. Coli RNase III under control of pBAD promoter and constitutively expresses gUTR-161-GFPDepositorInsertsRNase III
gUTR-161-GFP
UseTagsExpressionBacterialMutationN/APromoterJ23119 and pBADAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
PBDGTV
Plasmid#100859PurposeA piggyBac transposon based dual directional gene trap vector, in which, the mutagens are two non-selectable gene-trap cassettes in opposite orientations.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
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AV11_pDonor.EP.S1
Plasmid#100296PurposeAAVS1-targeting expression plasmidDepositorInsertPuroR.T2A.EGFP expression unit
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
hGR-Dx
Plasmid#133408Purposeoverexpression of a mutant human glucocorticoid receptor in mammalian cellsDepositorInsertNR3C1 (NR3C1 Human)
UseTagsExpressionMammalianMutationN454D, A458T, R460D, D462CPromoterAvailable sinceDec. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJin-264
Plasmid#125717PurposeABA-inducible split T7 gRNA for GFP vector using RNAPN d5-19DepositorInsertPT7-gRNA GFP expression, PYL-linker-T7 RNAPC, T7 RNAPN (d5-19)-linker-ABI
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZH051
Plasmid#47311PurposeExpresses Tsr-Venus-Ubiquitin-CIDepositorInsertwild type immunity region of lambda phage with tsr-venus-ub-cI
UseTagsCIExpressionBacterialMutationPromoterPR; PRMAvailable sinceSept. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJH579
Plasmid#172163PurposepylBCD variant in pBAD backboneDepositorInsertPara (pylBCD JM10.1); AraC
UseTagsExpressionBacterialMutationSumo(S6L) PylB(N61S, E84K, E122K, E175K, E178K, G…PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGDP3 arr
Plasmid#112890PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorInsertarr
UseTags6xHisExpressionBacterialMutationPromoterPblaAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGDP1 fosA
Plasmid#112889PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorInsertfosA
UseTags6xHisExpressionBacterialMutationPromoterPblaAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJin-239
Plasmid#125716PurposeABA-inducible split T7 gRNA for GFP vector using RNAPN N-29-1DepositorInsertpT7-gRNA GFP expression, PYL-linker-T7 RNAPC, T7 RNAPN (N-29-1)-linker-ABI
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
dSp-VP64-p65-RTA(75-190)
Plasmid#99670PurposeExpresses dSp Cas9 fused to combination of truncated activation domainsDepositorArticleInsertdCas9
UseTagsVP64-p65-RTA(75-190)ExpressionMammalianMutationdead Cas9PromoterAvailable sinceMay 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS1
Plasmid#55749PurposeProduces aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.DepositorInsertKanR
UseTagsExpressionBacterialMutationPromoterT7-lacAvailable sinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
DPY-pNCS
Plasmid#17365DepositorInsertMMLV proviral DNA
UseTagsExpressionMutationdeletion that removes 5 amino acids (DPPPY) from …PromoterAvailable sinceMarch 1, 2010AvailabilityAcademic Institutions and Nonprofits only