-
Plasmid#66158PurposeSix copies of the 415 bp PstI fragment of pCVD512, carrying the tdh1 gene probe from Vibrio parahaemolyticus WP1, for generation of probeDepositorInserttdh1
UseCloning vectorTagsExpressionMutationPromoterUnknownAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pU6-tevopreq1-GG-acceptor
Plasmid#174038PurposePrime editing in mammalian cellsDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterhU6Available sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
dead Sa Cas9
Plasmid#138162PurposeFor orthogonal R loop assay (use with Sp-derived base editor, Sp gRNA, and Sa gRNA for assay)DepositorInsertdSaCas9
UseTagsNLSExpressionMammalianMutationD10A + N580APromoterCMVAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
p5155
Plasmid#175391PurposeLow Copy Number DNA Donor Vector for Bxb1 Integrase-mediated Cassette Exchange (for inserts >15kb) into dual site mouse strains (Bxb-GT/GA)DepositorTypeEmpty backboneUseMouse Targeting; Cloning vector, to be used to de…TagsExpressionMutationPromoterAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU6-tmpknot-GG-acceptor
Plasmid#174039PurposePrime editing in mammalian cellsDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterhU6Available sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
dSp-VP64-p65-RTA(75-190)
Plasmid#99670PurposeExpresses dSp Cas9 fused to combination of truncated activation domainsDepositorArticleInsertdCas9
UseTagsVP64-p65-RTA(75-190)ExpressionMammalianMutationdead Cas9PromoterAvailable sinceMay 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMP11
Plasmid#215559PurposepKD46 with constitutively expressed Cas9 and an aTc gRNA targeting the ColE1 originDepositorInsertsCas9
gRNA targeting pBR322 origin of replication
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23105 and tetAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
AW01_pU.CAG.eSpCas9(1.1).rBGpA
Plasmid#199255PurposeExpression construct encoding the high specificity eSpCas9(1.1) nucleaseDepositorInsertHigh specificity eSpCas9(1.1) nuclease
UseCRISPRTags3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianMutationK848A K1003A R1060APromoterCAG promoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
AZ64_pE.DonorCLYBL.TS
Plasmid#199228PurposeDonor plasmid with CLYBL target sites for ITPN or HMEJ knock-in at the human CLYBL safe harbor locusDepositorInsertExpression unit for EGFP reporter
UseGene targeting donor plasmidTagsExpressionMammalianMutationPromoterHuman PGK1 gene promoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
ptrc99a-pVhb-tdTER-fdh
Plasmid#215649PurposepBR322 Origin, AmpR, with tdTER and Fdh genes under Vhb promoterDepositorInsertstrans-enoyl-CoA reductase
formate dehydrogenase
UseTagsExpressionBacterialMutationPromoterVhbAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pL2-1 ConjEpi TpEF2nat TpEF2NoSQEVenusThrFLAG
Plasmid#139961PurposeOver-expression of heterologus squalene epoxidase from Nannochloropsis oceanicaDepositorInsertConjEpi TpEF2_nat_tTpEF2 TpEF2_NoSQEVenusThrFLAG_tTpEF2
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
-
-
-
pRL25
Plasmid#70253PurposeShuttle vector suitable for transfer to Anabaena (Nostoc) sp. strain PCC 7120 and other cyanobacterial strainsDepositorTypeEmpty backboneUseBacterial shuttle vectorTagsExpressionMutationPromoterAvailable sinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSC-humanADAR1
Plasmid#198235PurposeExpresses human ADAR1 in yeastDepositorInserthuman ADAR1 (ADAR Human)
UseTagsHis10_TEVExpressionYeastMutationPromoterGAL1Available sinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
-
pGDP2 MCR-1
Plasmid#118404PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the pLac promoter.DepositorInsertMCR-1
UseTagsExpressionBacterialMutationPromoterpLacAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
-
pET30b_T7Sav
Plasmid#138588PurposeT7-tagged streptavidin with native C-terminus controlled by PT7 promoter; KanR, pBR322 oriDepositorInsertT7-tagged streptavidin variant with native C-terminus
UseSynthetic BiologyTagsT7-tagExpressionBacterialMutationPromoterPT7Available sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only